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The map label for this gene is rlmL [H]

Identifier: 222109559

GI number: 222109559

Start: 348065

End: 349378

Strand: Direct

Name: rlmL [H]

Synonym: Dtpsy_0339

Alternate gene names: 222109559

Gene position: 348065-349378 (Clockwise)

Preceding gene: 222109558

Following gene: 222109560

Centisome position: 9.17

GC content: 70.7

Gene sequence:

>1314_bases
ATGAACCAGCTTCAACTCTTTTTGCCCTGCGCCGCTGGCGTGGAGGGCTATCTGGCCGACGAGGTGCACAGCCTCACGGG
GCTGGCCGGGCAGGACCTGCTCGTGGGCCGCGCGGGCGTGCTGCTGCGCGCCTCGTGGCGCGATGCGCTGCGCCTGAACC
TGCACAGCCGCCTGGCGCAGCGCGTGCTGGTGCAACTGGCCGAGCGGCCCTACCGCAACGAGGACGACATCTATGCCGCC
GCGGCCGACGTGGCCTGGGAGATCTGGTTCACCCCGCGCCAGAGCTTCAAGGTGGAAGTGACGGCCCAGCACAGCCCGCT
CAAGAGCCTGAACTTCGCCGCGCTGCGCGTGAAGGATGCCGTGGCCGACCGCTTCCGCGCCAAGGCCGGCGTGCGTCCCG
ACGTGCAGACGCAGTGGCCCGACGTGCGCGTGCACCTGCACCTGACCAGCGACCGCGCCCAGATCTACATCGACACCTCG
GGAGAGCCGCTGTTCAAACGCGGCTGGCGCGAGGACAAGGGCGACGCGCCGCTCAAGGAAACGCTGGCCGCCGCCATGAT
CGCCGCCACCGGCTGGGACCCGCATGGCGAGCAGCCGCTGCCGCTGTACGACCCCTGCTGCGGTAGCGGCACCGTGGTCA
TCGAGGCCGCGCAGATCGCCTGCCGCATCGCGCCGGGCCTGCAGCGGCGCTTTGCCTTCGAGAAGCTATTGCCGCACCAG
GCGCATGTCTGGTCTGCTATCAAGGATGAAGCGGCCAGCGCCGTTACCGCGGCTTCGGTGCCGATTTTTGGCAGCGACGT
GTCGCACCGCATGGTGGACTTTGCCCAGCGCAATGCCGTGCGCGCCGGCGTGGCCGACGCGGTGCAGCTGCGCGGTGGCG
ACGCGTTGCAGCGCATGCCGCCCAGTGAACAACCCGGGGTGATGCTGCTGAATCCGCCGTATGGCGAGCGCATCGCCGCG
GCCGGCACGGCGGGGCGCAATGCGCGTGAGCGCATGTCCACGGTCGAGCGCATGGGCCGCGAGACGGCGCAGACTGAGGA
CGGCGTGGACTTCTTCGCGCAGTTGGCCGCGCACTGGAAGAAGCACTACGCCGGCTGGCAGGCCTGGATGCTCACGCCCG
ACCTCAAGCTGCCCAGCAAGATGCGCCTGAAGGAGTCGCGCCGCGTGCCGCTGTGGAACGGCCCCATCGAATGCCGCCTG
TTCCGCTTCGACCTCGTGGCGGGCGCCGTGCGGGATCGGCCGCGCGCGAGCCACACGGGGGAGGGCGGCGCGGCCAGCGG
TGCGCCGGCCGGCGGAGCCTCGGATGCGTCCTGA

Upstream 100 bases:

>100_bases
GCAAGACCGGCATCGTCTTCATCGACCGGGCGGCCCGCAAGCCCGCACCCATTCCTGAATCCGTGCTCCAGCCATTGCTG
CAGCGCGCAGCACAGCCGCA

Downstream 100 bases:

>100_bases
GCTGCGCCTGCCTGCCGTGGCGCCGGCGGGCGAGAGCGCGCGTGTCGTGGTGCTGGACACCAACATCGTGCTCGACCTGT
TCATCTTCGCCGACGCGGCC

Product: RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNQLQLFLPCAAGVEGYLADEVHSLTGLAGQDLLVGRAGVLLRASWRDALRLNLHSRLAQRVLVQLAERPYRNEDDIYAA
AADVAWEIWFTPRQSFKVEVTAQHSPLKSLNFAALRVKDAVADRFRAKAGVRPDVQTQWPDVRVHLHLTSDRAQIYIDTS
GEPLFKRGWREDKGDAPLKETLAAAMIAATGWDPHGEQPLPLYDPCCGSGTVVIEAAQIACRIAPGLQRRFAFEKLLPHQ
AHVWSAIKDEAASAVTAASVPIFGSDVSHRMVDFAQRNAVRAGVADAVQLRGGDALQRMPPSEQPGVMLLNPPYGERIAA
AGTAGRNARERMSTVERMGRETAQTEDGVDFFAQLAAHWKKHYAGWQAWMLTPDLKLPSKMRLKESRRVPLWNGPIECRL
FRFDLVAGAVRDRPRASHTGEGGAASGAPAGGASDAS

Sequences:

>Translated_437_residues
MNQLQLFLPCAAGVEGYLADEVHSLTGLAGQDLLVGRAGVLLRASWRDALRLNLHSRLAQRVLVQLAERPYRNEDDIYAA
AADVAWEIWFTPRQSFKVEVTAQHSPLKSLNFAALRVKDAVADRFRAKAGVRPDVQTQWPDVRVHLHLTSDRAQIYIDTS
GEPLFKRGWREDKGDAPLKETLAAAMIAATGWDPHGEQPLPLYDPCCGSGTVVIEAAQIACRIAPGLQRRFAFEKLLPHQ
AHVWSAIKDEAASAVTAASVPIFGSDVSHRMVDFAQRNAVRAGVADAVQLRGGDALQRMPPSEQPGVMLLNPPYGERIAA
AGTAGRNARERMSTVERMGRETAQTEDGVDFFAQLAAHWKKHYAGWQAWMLTPDLKLPSKMRLKESRRVPLWNGPIECRL
FRFDLVAGAVRDRPRASHTGEGGAASGAPAGGASDAS
>Mature_437_residues
MNQLQLFLPCAAGVEGYLADEVHSLTGLAGQDLLVGRAGVLLRASWRDALRLNLHSRLAQRVLVQLAERPYRNEDDIYAA
AADVAWEIWFTPRQSFKVEVTAQHSPLKSLNFAALRVKDAVADRFRAKAGVRPDVQTQWPDVRVHLHLTSDRAQIYIDTS
GEPLFKRGWREDKGDAPLKETLAAAMIAATGWDPHGEQPLPLYDPCCGSGTVVIEAAQIACRIAPGLQRRFAFEKLLPHQ
AHVWSAIKDEAASAVTAASVPIFGSDVSHRMVDFAQRNAVRAGVADAVQLRGGDALQRMPPSEQPGVMLLNPPYGERIAA
AGTAGRNARERMSTVERMGRETAQTEDGVDFFAQLAAHWKKHYAGWQAWMLTPDLKLPSKMRLKESRRVPLWNGPIECRL
FRFDLVAGAVRDRPRASHTGEGGAASGAPAGGASDAS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=371, Percent_Identity=30.9973045822102, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 47809; Mature: 47809

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLQLFLPCAAGVEGYLADEVHSLTGLAGQDLLVGRAGVLLRASWRDALRLNLHSRLAQ
CCCEEEEEEHHCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEECHHHHHHHHHHHHHHH
RVLVQLAERPYRNEDDIYAAAADVAWEIWFTPRQSFKVEVTAQHSPLKSLNFAALRVKDA
HHHHHHHHCCCCCCCCEEEEEHHEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VADRFRAKAGVRPDVQTQWPDVRVHLHLTSDRAQIYIDTSGEPLFKRGWREDKGDAPLKE
HHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCHHH
TLAAAMIAATGWDPHGEQPLPLYDPCCGSGTVVIEAAQIACRIAPGLQRRFAFEKLLPHQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCHHHHHHHHHHCCCH
AHVWSAIKDEAASAVTAASVPIFGSDVSHRMVDFAQRNAVRAGVADAVQLRGGDALQRMP
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCHHHCCC
PSEQPGVMLLNPPYGERIAAAGTAGRNARERMSTVERMGRETAQTEDGVDFFAQLAAHWK
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KHYAGWQAWMLTPDLKLPSKMRLKESRRVPLWNGPIECRLFRFDLVAGAVRDRPRASHTG
HHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCC
EGGAASGAPAGGASDAS
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQLQLFLPCAAGVEGYLADEVHSLTGLAGQDLLVGRAGVLLRASWRDALRLNLHSRLAQ
CCCEEEEEEHHCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEECHHHHHHHHHHHHHHH
RVLVQLAERPYRNEDDIYAAAADVAWEIWFTPRQSFKVEVTAQHSPLKSLNFAALRVKDA
HHHHHHHHCCCCCCCCEEEEEHHEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VADRFRAKAGVRPDVQTQWPDVRVHLHLTSDRAQIYIDTSGEPLFKRGWREDKGDAPLKE
HHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCHHH
TLAAAMIAATGWDPHGEQPLPLYDPCCGSGTVVIEAAQIACRIAPGLQRRFAFEKLLPHQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCHHHHHHHHHHCCCH
AHVWSAIKDEAASAVTAASVPIFGSDVSHRMVDFAQRNAVRAGVADAVQLRGGDALQRMP
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCHHHCCC
PSEQPGVMLLNPPYGERIAAAGTAGRNARERMSTVERMGRETAQTEDGVDFFAQLAAHWK
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KHYAGWQAWMLTPDLKLPSKMRLKESRRVPLWNGPIECRLFRFDLVAGAVRDRPRASHTG
HHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCC
EGGAASGAPAGGASDAS
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA