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The map label for this gene is rpoN [H]

Identifier: 222109557

GI number: 222109557

Start: 346044

End: 347621

Strand: Direct

Name: rpoN [H]

Synonym: Dtpsy_0337

Alternate gene names: 222109557

Gene position: 346044-347621 (Clockwise)

Preceding gene: 222109556

Following gene: 222109558

Centisome position: 9.11

GC content: 67.81

Gene sequence:

>1578_bases
ATGAAGCCCGGCCTGTCGCTGCGCGTCTCGCAGCACCTGGCGCTCACACCGCAGTTGCAGCAGTCGATCCGCCTGCTGCA
GCTGTCCACGCTGGAGCTGGCCCAGGAGGTCGAGCAAATGCTCGACGAGAACCCCTTCCTGGAGCGCACGCTGGAAGAGG
CCCCGCGCGAGGAGTTCGGCCTGGCCCAGGCCGATGCACCGGTGAGCGAGCAAGACCGCGAAGCCGAAGATGCTATTTAT
TCAGGAGCTGTCAGCGCAGGACAGGAAAGCGCTACAAGCCAAAATGATGCAGAAACCTCCTCCGCCGAATCCACGGCCGA
ACCCGCGGACTGGGAAGGCGACGGCTCGGTAGACCTGGCGCCCGACGACAGCGAGTGGGGCGGCGACGCCCCGGCACGCA
ACGGTGCCGGCGCGGGGAGCGACGACGAGGTGGACGCCACCGAGCTGGCCCGCAGCCAGGAGTCGCTCACGGCCTTCCTG
CACCGCCAGGCGCTGGCGCTGCGCCTGTCCGAGCTGGACATGGCCGCGCTGCGCTTCCTCATCGAATCGCTGAACGACGA
CGGCTACCTGGACGAGTCGCTGCAGGACCTGGCCGCCAGCCTGGCGGGCACCGAAGACCCCGAGCAACTCGATGAACTGG
TGCACCGTTTCACCGTGGCGCTGCGCCTGCTGCAAAGCCTGGAACCCGCGGGCGTGGGCGCGCGCGGCCTGGCCGAATGC
CTCACGCTGCAGCTGCGCGCGATGGACCAGGACGAAGAGGGCGACCCCGACGTGATCCAGGCCGCGCTGGCCATCTGCGG
CCAGCCACTGGACCTGCTGGCCAAGCGCGACGTGCGCCGCCTGGCGCAGGCCTGCGGCACCAGCGAAGAGCGCACGCGCG
CCGCGATGGCCTTGATCGCGCGGCTGGAGCCACGCCCGGGCAGGCGCTTTGCCGACGTGGACCGCAACATCATCGTGCCC
GACGTGATCGTCAAGAAAACCGGCCGCGGCGGACAGCAGAACTTCAGCGTGCAGCTCAACCCGGACGTGATGCCGCGTCT
GCGTGTGCACGACATCTACGCCGGCGCGTTGCGCGGCGGCCGTGGCGAAGGCCACCAGGCGCTGCAGGCACGGCTGCAGG
AGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTTGACACCATCCTGCGCGTGTCGCGCGCCATCGTGGAGCGGCAG
AAGAACTTCTTCGTGCATGGCGAACTGGCCATGCGCCCGCTGGTGCTGCGCGACATTGCCGATGAACTGGGCCTGCACGA
GTCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACGCCGCAAGGCACCTACGAACTCAAGTATTTCTTCGGCT
CGGGGCTGGGCACCGAAACCGGCGGCAACGCCTCCAGCACGGCCGTGCGCGCGCTCATCAAGCAGTTCATCGCGGCCGAG
AACCCGCACAAGCCGCTGTCGGACAGCCAGATCGCCGAGATGCTCAAGGAGCAGGGCATTGAATGTGCGCGCCGCACCGT
GGCCAAATACCGTGAGGCGCTCAAGATCGCCCCGGCCAACCTCCGCAAGGCACTTTGA

Upstream 100 bases:

>100_bases
AACGCGGAAGTGCGCCGGGTGTACCTGGGCGAGCACTTCCGCATGTGAGAGTGATGGGCGACCACGGCGCGATGCAGGAC
CAGACAGTGGGTTGCAGGCC

Downstream 100 bases:

>100_bases
ACCATGGCACGCGTCGTCTTCGACCTTCCCCCGCACTTCGGCTTCAGTACCGAGCTGCAGATCTACATCAGCCATGTCAA
CCAGGGCGGACACCTGGACA

Product: RNA polymerase sigma-54 subunit RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREEFGLAQADAPVSEQDREAEDAIY
SGAVSAGQESATSQNDAETSSAESTAEPADWEGDGSVDLAPDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFL
HRQALALRLSELDMAALRFLIESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
LTLQLRAMDQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIARLEPRPGRRFADVDRNIIVP
DVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGGRGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQ
KNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Sequences:

>Translated_525_residues
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREEFGLAQADAPVSEQDREAEDAIY
SGAVSAGQESATSQNDAETSSAESTAEPADWEGDGSVDLAPDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFL
HRQALALRLSELDMAALRFLIESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
LTLQLRAMDQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIARLEPRPGRRFADVDRNIIVP
DVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGGRGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQ
KNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL
>Mature_525_residues
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREEFGLAQADAPVSEQDREAEDAIY
SGAVSAGQESATSQNDAETSSAESTAEPADWEGDGSVDLAPDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFL
HRQALALRLSELDMAALRFLIESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
LTLQLRAMDQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIARLEPRPGRRFADVDRNIIVP
DVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGGRGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQ
KNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=526, Percent_Identity=40.4942965779468, Blast_Score=348, Evalue=4e-97,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 57422; Mature: 57422

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREEFG
CCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHC
LAQADAPVSEQDREAEDAIYSGAVSAGQESATSQNDAETSSAESTAEPADWEGDGSVDLA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECC
PDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFLHRQALALRLSELDMAALRFL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
HHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LTLQLRAMDQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIA
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RLEPRPGRRFADVDRNIIVPDVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGG
HCCCCCCCCHHHCCCCEECCHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
RGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQKNFFVHGELAMRPLVLRDIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHH
DELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
HHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREEFG
CCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHC
LAQADAPVSEQDREAEDAIYSGAVSAGQESATSQNDAETSSAESTAEPADWEGDGSVDLA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECC
PDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFLHRQALALRLSELDMAALRFL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
HHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LTLQLRAMDQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIA
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RLEPRPGRRFADVDRNIIVPDVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGG
HCCCCCCCCHHHCCCCEECCHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
RGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQKNFFVHGELAMRPLVLRDIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHH
DELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
HHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]