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The map label for this gene is phbI [H]

Identifier: 222109512

GI number: 222109512

Start: 301857

End: 303626

Strand: Direct

Name: phbI [H]

Synonym: Dtpsy_0292

Alternate gene names: 222109512

Gene position: 301857-303626 (Clockwise)

Preceding gene: 222109511

Following gene: 222109516

Centisome position: 7.95

GC content: 70.51

Gene sequence:

>1770_bases
ATGACCTTCTCCATCCACGGTCTGGCTGTCGCGCGCGGCATCGCCATCGGGCGGGCGGTGCTCGCGGTATCCAGCCGCAT
GGAGGTGGTGCACTACTTCATCGAGCCCGGCCAGGTCGAGGCAGAGATTCTGCGTGCGCGCCGCGGACGGGACGCGGTGG
CCGAGGAGCTGCAGCGCCTGCAGGCCGACATGCCGCACGACGCGCCGCCCGAGCTGGCCGCGCTGCTGGACGTGCACCTG
ATGCTGCTGCAGGACGAGATGCTGGTCAACGGCGTCAAGCACTGGATCACCGACCGCCTCTACAACGCCGAGTGGGCGCT
GACCACGCAGCTGGAGATCATCAGTCGCCAGTTCGACGAAATGGAGGACGACTACCTGCGCGAGCGCAAGGCCGACCTGG
AGCAGGTGGTGGAGCGCATCCTGCGCCACATGCGCGGCGTGGCCAGCCCCGTCGCGCCGCCGCCCTCCGCCCCCCGTCGC
CAGGCCGAGGCGGGTGCGGAGGCGGAGTTCCTGCTGGGCGGCGCGGCCGATGCGCCGCTGGTGCTGGTGGCGCACGATTT
GTCGCCGGCCGACATGCTGCAGTTCAAGCAGAGCGTGTTCGCCGGCTTCGTGACCGACGTGGGGGGCAAGACCAGCCACA
CGGCCATCGTGGCGCGCAGCATGGACATCCCCGCCGTGGTGGGTGCGCGCAGCGCCAGCCAGTTGGTGCGACAGGACGAC
TGGGTCATCATCGACGGCGATGCCGGCGTGGTCATCGTCGACCCCACGCCGATCATCCTGGCGGAATACGGCTTTCGCCA
GCGCCAGATCACGCTGGAGCGCGAGCGCCTCATGCGGCTGCGCCACACGCCGGCCGTCACGCTGGATGCACAGGCCATTG
AGCTGCTGGCCAACATCGAGCAGCCCGGTGATGCGGGTGCCGCGCTGCGCGCGGGCGCGGCGGGCGTGGGGTTGTTTCGC
AGCGAATTTTTGTTCATGGGCCGCAGCGGCCGCCTGCCGGACGAGGAGGAGCAGTACCGCGCCTACCGCGAGGCGGTGGA
GGGCATGCAGGGCCTGCCCGTGACCATCCGCACCGTCGATGTGGGCGCCGACAAGCCGCTGGACAAGGGGTACAAGGACA
GTTCGCTCAACCCGGCGCTGGGCCTGCGCGCCATCCGCTGGAGCCTGGCCGATCCGGCCATGTTCCGCACCCAGCTGCGC
GCGGTGCTGCGCGCGGCGGCACACGGCCCGGTGCGGCTGCTGTTCCCCATGCTCGCGCATGTGAGCGAGATCCGGCAGAC
GCTGGCCCAGGTGGACCTGGCGCGCGCGGAGCTGGACGCACGCAGCGCTGCCTACGGTGCGGTGCAGCTGGGCGCCATGA
TCGAGGTGCCGGCCGCTGCGCTCATGGTGCGCCAGTTCCTGCGCTACTTCGACTTTCTCTCGATCGGCACCAACGACCTG
ATCCAGTACGCGCTGGCCATCGACCGCGCGGACGAGGCCGTGGCCCACCTGTACGACCCGTTGCACCCGGCCGTGCTGCG
GCTGGTGGCCGACGTGATCGCGGAGGGCGCGCGCCAGGGCAAGAGCGTGAGCGTGTGCGGCGAGATGGCGGGCGATGTCG
CCATGACCCGGCTGCTGCTGGGCCTGGGCCTGCGCAGCTTCTCCATGCAGCCGGCGCAGATCCTGGCCGTCAAGCAGGAG
GTGCTGCGCGCCGACGCGCGCAAGCTGGGCGAGTGGGCGCAGCAACTGTTGGATGCGGACGATCCGGCTGCGTCGTTGCA
GGCGCCTTGA

Upstream 100 bases:

>100_bases
CGAGACCGATGGCGCGCAGGAGCAGGAAGCCATGGATGCGCTTGTCGCGCTCATCGATGGCAAGTTCGGCGAGGGCGAAT
AAACCGCAGGCGAGGCACGC

Downstream 100 bases:

>100_bases
TCGCTATTTAAATAATAGCTGCTGGCGCTTGTCAGTATTGACCTAGAGGCCGATTTGACTTGTATTCCGAGTCAAACGCC
AGCGGCGTGCGCCTGCTGGT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTFSIHGLAVARGIAIGRAVLAVSSRMEVVHYFIEPGQVEAEILRARRGRDAVAEELQRLQADMPHDAPPELAALLDVHL
MLLQDEMLVNGVKHWITDRLYNAEWALTTQLEIISRQFDEMEDDYLRERKADLEQVVERILRHMRGVASPVAPPPSAPRR
QAEAGAEAEFLLGGAADAPLVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARSASQLVRQDD
WVIIDGDAGVVIVDPTPIILAEYGFRQRQITLERERLMRLRHTPAVTLDAQAIELLANIEQPGDAGAALRAGAAGVGLFR
SEFLFMGRSGRLPDEEEQYRAYREAVEGMQGLPVTIRTVDVGADKPLDKGYKDSSLNPALGLRAIRWSLADPAMFRTQLR
AVLRAAAHGPVRLLFPMLAHVSEIRQTLAQVDLARAELDARSAAYGAVQLGAMIEVPAAALMVRQFLRYFDFLSIGTNDL
IQYALAIDRADEAVAHLYDPLHPAVLRLVADVIAEGARQGKSVSVCGEMAGDVAMTRLLLGLGLRSFSMQPAQILAVKQE
VLRADARKLGEWAQQLLDADDPAASLQAP

Sequences:

>Translated_589_residues
MTFSIHGLAVARGIAIGRAVLAVSSRMEVVHYFIEPGQVEAEILRARRGRDAVAEELQRLQADMPHDAPPELAALLDVHL
MLLQDEMLVNGVKHWITDRLYNAEWALTTQLEIISRQFDEMEDDYLRERKADLEQVVERILRHMRGVASPVAPPPSAPRR
QAEAGAEAEFLLGGAADAPLVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARSASQLVRQDD
WVIIDGDAGVVIVDPTPIILAEYGFRQRQITLERERLMRLRHTPAVTLDAQAIELLANIEQPGDAGAALRAGAAGVGLFR
SEFLFMGRSGRLPDEEEQYRAYREAVEGMQGLPVTIRTVDVGADKPLDKGYKDSSLNPALGLRAIRWSLADPAMFRTQLR
AVLRAAAHGPVRLLFPMLAHVSEIRQTLAQVDLARAELDARSAAYGAVQLGAMIEVPAAALMVRQFLRYFDFLSIGTNDL
IQYALAIDRADEAVAHLYDPLHPAVLRLVADVIAEGARQGKSVSVCGEMAGDVAMTRLLLGLGLRSFSMQPAQILAVKQE
VLRADARKLGEWAQQLLDADDPAASLQAP
>Mature_588_residues
TFSIHGLAVARGIAIGRAVLAVSSRMEVVHYFIEPGQVEAEILRARRGRDAVAEELQRLQADMPHDAPPELAALLDVHLM
LLQDEMLVNGVKHWITDRLYNAEWALTTQLEIISRQFDEMEDDYLRERKADLEQVVERILRHMRGVASPVAPPPSAPRRQ
AEAGAEAEFLLGGAADAPLVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARSASQLVRQDDW
VIIDGDAGVVIVDPTPIILAEYGFRQRQITLERERLMRLRHTPAVTLDAQAIELLANIEQPGDAGAALRAGAAGVGLFRS
EFLFMGRSGRLPDEEEQYRAYREAVEGMQGLPVTIRTVDVGADKPLDKGYKDSSLNPALGLRAIRWSLADPAMFRTQLRA
VLRAAAHGPVRLLFPMLAHVSEIRQTLAQVDLARAELDARSAAYGAVQLGAMIEVPAAALMVRQFLRYFDFLSIGTNDLI
QYALAIDRADEAVAHLYDPLHPAVLRLVADVIAEGARQGKSVSVCGEMAGDVAMTRLLLGLGLRSFSMQPAQILAVKQEV
LRADARKLGEWAQQLLDADDPAASLQAP

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=36.8237347294939, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1789193, Length=532, Percent_Identity=34.5864661654135, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI48994992, Length=504, Percent_Identity=32.9365079365079, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1788726, Length=583, Percent_Identity=30.3602058319039, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1787994, Length=401, Percent_Identity=26.4339152119701, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI226510935, Length=185, Percent_Identity=24.8648648648649, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64292; Mature: 64161

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSIHGLAVARGIAIGRAVLAVSSRMEVVHYFIEPGQVEAEILRARRGRDAVAEELQRL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
QADMPHDAPPELAALLDVHLMLLQDEMLVNGVKHWITDRLYNAEWALTTQLEIISRQFDE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
MEDDYLRERKADLEQVVERILRHMRGVASPVAPPPSAPRRQAEAGAEAEFLLGGAADAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCE
VLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARSASQLVRQDD
EEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHCCC
WVIIDGDAGVVIVDPTPIILAEYGFRQRQITLERERLMRLRHTPAVTLDAQAIELLANIE
EEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCC
QPGDAGAALRAGAAGVGLFRSEFLFMGRSGRLPDEEEQYRAYREAVEGMQGLPVTIRTVD
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
VGADKPLDKGYKDSSLNPALGLRAIRWSLADPAMFRTQLRAVLRAAAHGPVRLLFPMLAH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VSEIRQTLAQVDLARAELDARSAAYGAVQLGAMIEVPAAALMVRQFLRYFDFLSIGTNDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IQYALAIDRADEAVAHLYDPLHPAVLRLVADVIAEGARQGKSVSVCGEMAGDVAMTRLLL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
GLGLRSFSMQPAQILAVKQEVLRADARKLGEWAQQLLDADDPAASLQAP
HHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TFSIHGLAVARGIAIGRAVLAVSSRMEVVHYFIEPGQVEAEILRARRGRDAVAEELQRL
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
QADMPHDAPPELAALLDVHLMLLQDEMLVNGVKHWITDRLYNAEWALTTQLEIISRQFDE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
MEDDYLRERKADLEQVVERILRHMRGVASPVAPPPSAPRRQAEAGAEAEFLLGGAADAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCE
VLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARSASQLVRQDD
EEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHCCC
WVIIDGDAGVVIVDPTPIILAEYGFRQRQITLERERLMRLRHTPAVTLDAQAIELLANIE
EEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCC
QPGDAGAALRAGAAGVGLFRSEFLFMGRSGRLPDEEEQYRAYREAVEGMQGLPVTIRTVD
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
VGADKPLDKGYKDSSLNPALGLRAIRWSLADPAMFRTQLRAVLRAAAHGPVRLLFPMLAH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VSEIRQTLAQVDLARAELDARSAAYGAVQLGAMIEVPAAALMVRQFLRYFDFLSIGTNDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IQYALAIDRADEAVAHLYDPLHPAVLRLVADVIAEGARQGKSVSVCGEMAGDVAMTRLLL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
GLGLRSFSMQPAQILAVKQEVLRADARKLGEWAQQLLDADDPAASLQAP
HHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]