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The map label for this gene is tuf1 [H]

Identifier: 222109491

GI number: 222109491

Start: 289235

End: 290425

Strand: Direct

Name: tuf1 [H]

Synonym: Dtpsy_0271

Alternate gene names: 222109491

Gene position: 289235-290425 (Clockwise)

Preceding gene: 222109490

Following gene: 222109492

Centisome position: 7.62

GC content: 63.64

Gene sequence:

>1191_bases
ATGGCAAAAGGTAAGTTTGAACGTACCAAGCCCCACGTGAACGTGGGCACGATCGGTCACGTGGACCATGGCAAGACGAC
GCTGACGGCGGCCATCGCCACGGTGCTGTCCAAGAAGTTCGGCGGCGAAGCCAAGGGCTACGACCAGATCGACAACGCCC
CTGAAGAAAAGGCGCGCGGCATCACGATCAACACCTCGCACGTGGAATACGAAACGGCCACCCGCCACTACGCCCACGTC
GACTGCCCCGGCCACGCCGACTACGTCAAGAACATGATCACCGGCGCCGCTCAGATGGACGGCGCCATCCTGGTGTGCTC
GGCCGCTGACGGCCCGATGCCCCAGACGCGCGAGCACATCCTGCTGGCCCGTCAGGTGGGCGTGCCCTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTCGACGACGAGGAACTGCTCGAGCTGGTCGAGATGGAAGTGCGTGAACTGCTGGACAAG
TACGACTTCCCCGGCGACGACACCCCCATCATCCGCGGCTCCGCCAAGCTGGCCCTGGAAGGTGACCAGTCCGACAAGGG
CGAACCCGCCATCATGCGCCTGGCCGAAGCCCTGGACACCTACATCCCCACGCCTGAGCGCGCCGTGGACGGCGCCTTCC
TGATGCCTGTGGAAGACGTGTTCTCCATCTCCGGCCGCGGCACCGTGGTGACCGGCCGCGTCGAGCGCGGCATCATCAAG
GTCGGCGAAGAAATCGAAATCGTCGGTATCCGCGACACGCAAAAGACCACCGTCACCGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAGGGCCAGGCTGGCGACAACGTGGGCCTGCTGCTGCGCGGCACCAAGCGCGAAGACGTCGAGCGCGGCCAAG
TGCTGTGCAAGCCCGGCTCCATCAAGCCCCACACCCACTTCACGGCTGAGGTGTACGTGCTGAGCAAGGACGAAGGCGGT
CGTCACACGCCCTTCTTCAACAACTACCGTCCCCAGTTCTACTTCCGCACCACCGACGTGACCGGCTCCATCGAGCTGCC
GGCCGACAAGGAAATGGTGATGCCCGGCGACAACGTGTCGATCACTGTGAAGCTGATCAACCCCATCGCCATGGAAGAAG
GCCTGCGCTTCGCTATCCGCGAAGGCGGCCGTACCGTGGGCGCCGGCGTCGTTGCCAAGATCATTGCGTAA

Upstream 100 bases:

>100_bases
CTGCGATCCGGTGCCGTCCTGTTGCACTGTGCGGGCCGAACCAGCAACCGGATGCAGACGTTAAACCCTCACACAGGCAT
TGCTCTTTTGGAGTTCGAAA

Downstream 100 bases:

>100_bases
TCCAGCAAAGCACTGAAGGAATACACAATGTCCAAGCAAAAGATCCGCATCCGCCTGAAGGCATTCGACTACAAGCTGAT
CGATCAGTCTGCAGCCGAGA

Product: elongation factor tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKGYDQIDNAPEEKARGITINTSHVEYETATRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDK
YDFPGDDTPIIRGSAKLALEGDQSDKGEPAIMRLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
VGEEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKGYDQIDNAPEEKARGITINTSHVEYETATRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDK
YDFPGDDTPIIRGSAKLALEGDQSDKGEPAIMRLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
VGEEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA
>Mature_395_residues
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKGYDQIDNAPEEKARGITINTSHVEYETATRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDKY
DFPGDDTPIIRGSAKLALEGDQSDKGEPAIMRLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIKV
GEEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTAEVYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.9620253164557, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=432, Percent_Identity=31.25, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI5729864, Length=432, Percent_Identity=31.25, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.6875, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.4642857142857, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI194018522, Length=430, Percent_Identity=28.8372093023256, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI46094014, Length=435, Percent_Identity=29.1954022988506, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI194018520, Length=430, Percent_Identity=28.8372093023256, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI194097354, Length=430, Percent_Identity=28.8372093023256, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI54607086, Length=330, Percent_Identity=29.0909090909091, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4503507, Length=275, Percent_Identity=28.7272727272727, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.1111111111111, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=81.3131313131313, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=81.3131313131313, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=30.4733727810651, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI48994988, Length=244, Percent_Identity=31.5573770491803, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789108, Length=322, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=53.1017369727047, Blast_Score=413, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.1860465116279, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.5803571428571, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.5803571428571, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.3410138248848, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=304, Percent_Identity=30.9210526315789, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=349, Percent_Identity=29.7994269340974, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=26.3038548752834, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=26.3038548752834, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=36.734693877551, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=275, Percent_Identity=26.9090909090909, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=28.1512605042017, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=65.5696202531646, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=30, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=30, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=30.0884955752212, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=26.4957264957265, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=268, Percent_Identity=27.9850746268657, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.5241730279898, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.5241730279898, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=52.0725388601036, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.4642857142857, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.4642857142857, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137380, Length=340, Percent_Identity=31.4705882352941, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=28.4403669724771, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=29.8360655737705, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647046, Length=268, Percent_Identity=28.7313432835821, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=268, Percent_Identity=28.7313432835821, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43262; Mature: 43131

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKGYDQIDNAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHCC
ITINTSHVEYETATRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDKYDFPGDDTPIIRGSAKLALE
HEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDQSDKGEPAIMRLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
CCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECHHHCCCEE
VGEEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA
EEEEEECCHHHHHCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKGYDQIDNAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHCC
ITINTSHVEYETATRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDKYDFPGDDTPIIRGSAKLALE
HEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDQSDKGEPAIMRLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
CCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECHHHCCCEE
VGEEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA
EEEEEECCHHHHHCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA