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The map label for this gene is glcE [H]

Identifier: 222109405

GI number: 222109405

Start: 207176

End: 208285

Strand: Direct

Name: glcE [H]

Synonym: Dtpsy_0184

Alternate gene names: 222109405

Gene position: 207176-208285 (Clockwise)

Preceding gene: 222109404

Following gene: 222109406

Centisome position: 5.46

GC content: 72.25

Gene sequence:

>1110_bases
ATGGACGCAGCCCTTCGCCAGATCATCGACCGCGTGCGCGACAGCGCAGACCGCCGCAGCGCCGTGCGCATCTGCGGCGG
AGGCACCAAAGACTTCCATGGCCCGGGCGCGCCGCAGCATGCCGGTGAGCCGCTGGACATGCGGCCCCTGGCCGGCATCG
TGAGCTACGAGCCCAGCGAACTGGTGGTGACCGCGCTCGCCGGCACGCCGCTGGCAGAGCTGGAGGCGCTGCTCGCGCGC
CATGGCCAGAGCCTGCCGTTCGAGCCGCCACGCTTTGCCGAAGGCGGCACCGTGGGGGGCATGGTGGCGGCCGGCCTGTC
GGGGCCGGCGCGCGCCAGCGTGGGCGCGGTGCGCGACTACGTGCTGGGCGTGGAGATCATCAACGGGCGCGGCGAGCTGC
TGCGCTTTGGCGGGCAGGTGATGAAGAACGTGGCGGGCTATGACGTCTCGCGCCTCATGGCCGGGGCCTGGGGGCAGCTC
GGCGTGATCACCGAGGTCAGCCTGAAGGTGCTGCCGGTGCCGCCGGCGGAGGCCACGCTGCGCTTTGACTGCGACCAGGC
CGAGGCGCTGCGCCGCCTCAACGCCTGGGGCGGCCAGCCGCTGCCGCTCAACGCCAGCTGCTGGGTCGAGGATGCGGGCC
GCGGCCAGCTCTACGTGCGCCTGCGTGGCGCGCAGGCGGCGGTGCAGGCCGCCTGCCGCAGCATGGGAGGTGAGTTGCAA
GACGCTCCCACCGTGCCGGACGACTGGCAGGCTTGCCGCGACCAGCGCCTGCCCTGGTTCGCCGCGCGCGCGGCGGACCA
TGCGCTGTGGCGCCTGTCGGTTCCCCAGACCGCGCCCGTGCTGGCATTACCCGCGGGCGTGGCGCCGCCGCTGGTGGAGT
GGCACGGCGGTCTGCGCTGGGTGCAGGCGCTGCCGCAGCACGGCGATGCGCTGCACGCACTGGCCGCCGAGGTGGGGGGC
AGTGCTTCTCTATTCATAGCTGCTAGCGCAGATGGGATAAGCGCTAGAGCCATTTTTGATGCAAATTCTGCGGCGCTCGC
TGCGATCCACCAGCGGCTCAAGCAGGCGTTCGACCCGGCCGGCATCTTCAACCCCGGGCGCCTCGTCTAG

Upstream 100 bases:

>100_bases
CTGCGGCGCGCATGGCTGCCAGCGCCATTCACGCCGCGCGTTCAAAAAAATCAAACCTGCGCCACGGCCGCCCGAAAGGG
CAGGGCAGTACCATCGCCGG

Downstream 100 bases:

>100_bases
CCATGCGCCTGCTGCGCCACCTGACCGGGCGGCACCGCACCACGGTCACCAACCGCACGCTGGGCCTGCTGCTGGCGTTC
AACGCGGGGGCGATCAACGC

Product: fad linked oxidase domain-containing protein

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MDAALRQIIDRVRDSADRRSAVRICGGGTKDFHGPGAPQHAGEPLDMRPLAGIVSYEPSELVVTALAGTPLAELEALLAR
HGQSLPFEPPRFAEGGTVGGMVAAGLSGPARASVGAVRDYVLGVEIINGRGELLRFGGQVMKNVAGYDVSRLMAGAWGQL
GVITEVSLKVLPVPPAEATLRFDCDQAEALRRLNAWGGQPLPLNASCWVEDAGRGQLYVRLRGAQAAVQAACRSMGGELQ
DAPTVPDDWQACRDQRLPWFAARAADHALWRLSVPQTAPVLALPAGVAPPLVEWHGGLRWVQALPQHGDALHALAAEVGG
SASLFIAASADGISARAIFDANSAALAAIHQRLKQAFDPAGIFNPGRLV

Sequences:

>Translated_369_residues
MDAALRQIIDRVRDSADRRSAVRICGGGTKDFHGPGAPQHAGEPLDMRPLAGIVSYEPSELVVTALAGTPLAELEALLAR
HGQSLPFEPPRFAEGGTVGGMVAAGLSGPARASVGAVRDYVLGVEIINGRGELLRFGGQVMKNVAGYDVSRLMAGAWGQL
GVITEVSLKVLPVPPAEATLRFDCDQAEALRRLNAWGGQPLPLNASCWVEDAGRGQLYVRLRGAQAAVQAACRSMGGELQ
DAPTVPDDWQACRDQRLPWFAARAADHALWRLSVPQTAPVLALPAGVAPPLVEWHGGLRWVQALPQHGDALHALAAEVGG
SASLFIAASADGISARAIFDANSAALAAIHQRLKQAFDPAGIFNPGRLV
>Mature_369_residues
MDAALRQIIDRVRDSADRRSAVRICGGGTKDFHGPGAPQHAGEPLDMRPLAGIVSYEPSELVVTALAGTPLAELEALLAR
HGQSLPFEPPRFAEGGTVGGMVAAGLSGPARASVGAVRDYVLGVEIINGRGELLRFGGQVMKNVAGYDVSRLMAGAWGQL
GVITEVSLKVLPVPPAEATLRFDCDQAEALRRLNAWGGQPLPLNASCWVEDAGRGQLYVRLRGAQAAVQAACRSMGGELQ
DAPTVPDDWQACRDQRLPWFAARAADHALWRLSVPQTAPVLALPAGVAPPLVEWHGGLRWVQALPQHGDALHALAAEVGG
SASLFIAASADGISARAIFDANSAALAAIHQRLKQAFDPAGIFNPGRLV

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=361, Percent_Identity=43.213296398892, Blast_Score=275, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6320027, Length=163, Percent_Identity=26.9938650306748, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320764, Length=177, Percent_Identity=25.9887005649718, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 38753; Mature: 38753

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAALRQIIDRVRDSADRRSAVRICGGGTKDFHGPGAPQHAGEPLDMRPLAGIVSYEPSE
CCHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
LVVTALAGTPLAELEALLARHGQSLPFEPPRFAEGGTVGGMVAAGLSGPARASVGAVRDY
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHH
VLGVEIINGRGELLRFGGQVMKNVAGYDVSRLMAGAWGQLGVITEVSLKVLPVPPAEATL
HEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEEEECCCCCCEE
RFDCDQAEALRRLNAWGGQPLPLNASCWVEDAGRGQLYVRLRGAQAAVQAACRSMGGELQ
EECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCC
DAPTVPDDWQACRDQRLPWFAARAADHALWRLSVPQTAPVLALPAGVAPPLVEWHGGLRW
CCCCCCHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCCCEEEECCCCCCCHHHHHCCHHH
VQALPQHGDALHALAAEVGGSASLFIAASADGISARAIFDANSAALAAIHQRLKQAFDPA
HHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
GIFNPGRLV
CCCCCCCCC
>Mature Secondary Structure
MDAALRQIIDRVRDSADRRSAVRICGGGTKDFHGPGAPQHAGEPLDMRPLAGIVSYEPSE
CCHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
LVVTALAGTPLAELEALLARHGQSLPFEPPRFAEGGTVGGMVAAGLSGPARASVGAVRDY
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHH
VLGVEIINGRGELLRFGGQVMKNVAGYDVSRLMAGAWGQLGVITEVSLKVLPVPPAEATL
HEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEEEECCCCCCEE
RFDCDQAEALRRLNAWGGQPLPLNASCWVEDAGRGQLYVRLRGAQAAVQAACRSMGGELQ
EECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCC
DAPTVPDDWQACRDQRLPWFAARAADHALWRLSVPQTAPVLALPAGVAPPLVEWHGGLRW
CCCCCCHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCCCEEEECCCCCCCHHHHHCCHHH
VQALPQHGDALHALAAEVGGSASLFIAASADGISARAIFDANSAALAAIHQRLKQAFDPA
HHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
GIFNPGRLV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]