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The map label for this gene is gidB

Identifier: 222109271

GI number: 222109271

Start: 50395

End: 51075

Strand: Direct

Name: gidB

Synonym: Dtpsy_0046

Alternate gene names: 222109271

Gene position: 50395-51075 (Clockwise)

Preceding gene: 222109270

Following gene: 222109272

Centisome position: 1.33

GC content: 65.05

Gene sequence:

>681_bases
ATGAGCGTGGTGTCGAACACAAGCCTTGAGCACGCCTTGCGTGAGGGCGCCAACGCCTTGGGGTTGGATCTGTCGGAAGC
GCAAATCACACAGCTATTGGACTTCCTGGCGCTGCTGCAAAAGTGGAACAAGGTCTACAACCTCACGGCGGTGCGCGATC
CGCAGGAAATGCTCACGCACCACCTGCTGGACAGCCTGGCTGCCGTCCCGCCGCTGCGCCGCCATGTCGCGCGGAGGGGG
CAGGGCGGTGCAATCCACCTGGGCGCGCGGCTGCTGGATGTGGGATCAGGCGGGGGACTGCCTGGCGTGGTGTTCGCCAT
TTGTTGCCCGGAAGTGGATGTGAGTTGCGTGGACACCGTAGCCAAGAAAGCAGCATTCATCCAGCAGGCGGCGGGCACCC
TCGGTCTGTCCAACCTACATGGCATCCATGCGCGCGTGGAAACGCTGGCCGGTCCGTTCGATGTGGTCAGTTGCCGCGCC
TTTGCCGCGCTGGCGGACTTCACCGCCTGGTCGCGCCAGGCGCTGGCACCGCACGGCGTGTGGCTGGCCATGAAGGGAAA
GCACCCGCACGACGAGATTGCCGCGCTACCGGCGGACGTAAGCGTGTTTCACGTGGAACAACTCACTGTGCCGGGCCTGC
AGGCCGAACGCTGCATCCTCTGGCTGCGCCCCGTCGCGTAA

Upstream 100 bases:

>100_bases
GGGTGACACCGGCGGCGATCTCGCTGCTGATGGTCCACTTGAAGAAGGGCGGTTTCAAGGGGTTTGCCCCGCAGCCGGCG
GATGGGGTGGAGACGGTCGC

Downstream 100 bases:

>100_bases
ACTCAAGCCCCCTCCCGCTTCGCAAGGCGCTCCCATGTTCGGCATTGCAGACTACGGCGCATTCGTCGCCGCCATCGTGT
TGTTCCTCGCCATCCCGGGG

Product: 16S rRNA methyltransferase gidb

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSVVSNTSLEHALREGANALGLDLSEAQITQLLDFLALLQKWNKVYNLTAVRDPQEMLTHHLLDSLAAVPPLRRHVARRG
QGGAIHLGARLLDVGSGGGLPGVVFAICCPEVDVSCVDTVAKKAAFIQQAAGTLGLSNLHGIHARVETLAGPFDVVSCRA
FAALADFTAWSRQALAPHGVWLAMKGKHPHDEIAALPADVSVFHVEQLTVPGLQAERCILWLRPVA

Sequences:

>Translated_226_residues
MSVVSNTSLEHALREGANALGLDLSEAQITQLLDFLALLQKWNKVYNLTAVRDPQEMLTHHLLDSLAAVPPLRRHVARRG
QGGAIHLGARLLDVGSGGGLPGVVFAICCPEVDVSCVDTVAKKAAFIQQAAGTLGLSNLHGIHARVETLAGPFDVVSCRA
FAALADFTAWSRQALAPHGVWLAMKGKHPHDEIAALPADVSVFHVEQLTVPGLQAERCILWLRPVA
>Mature_225_residues
SVVSNTSLEHALREGANALGLDLSEAQITQLLDFLALLQKWNKVYNLTAVRDPQEMLTHHLLDSLAAVPPLRRHVARRGQ
GGAIHLGARLLDVGSGGGLPGVVFAICCPEVDVSCVDTVAKKAAFIQQAAGTLGLSNLHGIHARVETLAGPFDVVSCRAF
AALADFTAWSRQALAPHGVWLAMKGKHPHDEIAALPADVSVFHVEQLTVPGLQAERCILWLRPVA

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=205, Percent_Identity=39.0243902439024, Blast_Score=158, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_ACIET (B9M9W4)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002551535.1
- ProteinModelPortal:   B9M9W4
- GeneID:   7382737
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_0046
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 24129; Mature: 23998

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVSNTSLEHALREGANALGLDLSEAQITQLLDFLALLQKWNKVYNLTAVRDPQEMLTH
CCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHH
HLLDSLAAVPPLRRHVARRGQGGAIHLGARLLDVGSGGGLPGVVFAICCPEVDVSCVDTV
HHHHHHHHCCHHHHHHHHCCCCCEEEECCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
AKKAAFIQQAAGTLGLSNLHGIHARVETLAGPFDVVSCRAFAALADFTAWSRQALAPHGV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
WLAMKGKHPHDEIAALPADVSVFHVEQLTVPGLQAERCILWLRPVA
EEEECCCCCHHHHHHCCCCCEEEEEEHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
SVVSNTSLEHALREGANALGLDLSEAQITQLLDFLALLQKWNKVYNLTAVRDPQEMLTH
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHH
HLLDSLAAVPPLRRHVARRGQGGAIHLGARLLDVGSGGGLPGVVFAICCPEVDVSCVDTV
HHHHHHHHCCHHHHHHHHCCCCCEEEECCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
AKKAAFIQQAAGTLGLSNLHGIHARVETLAGPFDVVSCRAFAALADFTAWSRQALAPHGV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
WLAMKGKHPHDEIAALPADVSVFHVEQLTVPGLQAERCILWLRPVA
EEEECCCCCHHHHHHCCCCCEEEEEEHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA