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The map label for this gene is nac [H]

Identifier: 222102911

GI number: 222102911

Start: 596173

End: 597078

Strand: Reverse

Name: nac [H]

Synonym: Avi_7661

Alternate gene names: 222102911

Gene position: 597078-596173 (Counterclockwise)

Preceding gene: 222102919

Following gene: 222102902

Centisome position: 94.51

GC content: 57.06

Gene sequence:

>906_bases
ATGGATATCAGGCGCTTCAAATCCTTCATCGTGATTGTCGATACAGGCAGCATTACCCGTGCGGCCGATATTCTGCATAT
CGCTCAGCCCGCCTTGAGCCAGCAACTTGCCTCGCTGGAAGAACATTTCGGCACGAAGCTTTTGCTGCGCAGCCAGCAAG
GCGTGGTGATGACCGATGCGGGCGCGCAAGTCTATCGTCATGCGCAGATGATTCTGCGGCAGATGGATCAGGCCCATGCC
GATGTGCTGGCCGCAGGCAAGCATTTGGCCGGGCGGGTCTCGGTGGGTCTGGCTCCGTTTAGCAGCGCGGCAACGCTCTC
GTTTGAGTTGCTGGCGGAAACACGCAGGCTTTATCCGGGCATTGTGCTGCATCTGACCGAAAGCGTGGGGCAGGCCTATA
GCCAAATGTTGATGAACAACAAGCTTGAAATCGCCCTCATCCACGGCACCGGACCCATGAAGGGTGTTCACTTCGATCCT
TTACTGCGCGAAGATTTCTTTCTGGTGGCCCATGAGGATTTTGGCATTGAGGCCGATGACAAGCCGCTCTCGGTCGCCAA
TCTGGAAGAGCTGCCTTTTCTGCTGCCGCCGCCGTATAATTTTGTGCGCCGTGCCGTGGATATGGCCTTTGCCCGCAGCC
GTACCAAGCTGAACGTGATTGGCGAGGTAGAGGTTATTCGCACCCTGACGCGGGCAGTCAGTGGCGGTGTTGGGGCAACT
ATCATGCCCAAGGCGGTGGCTGATCGCATCACCGCAGAGGCAAGCAGCCAGATCATCTGCCGCACCCTGTCGCCGCAGAT
TGAAGAAACCCTGTCACTCTGTGTGTCTGAACACGCGCAATTGTCAGACTCAGCCGTGGCCGTCAGGGATATTTTGCTGC
GTTTGACGCGGCAGTTGAAGAATTAG

Upstream 100 bases:

>100_bases
CGCCAAAGCCAGCTCAAAACCTTGTTTTATCCATTTACGGCGTGACGGCTGCTGCATAGTGTGAAGGCTTGACCCCATAG
AATTCGAGTTTACTTCCCCC

Downstream 100 bases:

>100_bases
AGCGGTTTATGCGCGCCATGCATCCGTTGGGATGCATGGTGCAATATTTTCTACATGGCCAAGATTACTTCATATCCTTG
CAGAATTGCGCAATCCGGTC

Product: transcriptional regulator LysR family

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDIRRFKSFIVIVDTGSITRAADILHIAQPALSQQLASLEEHFGTKLLLRSQQGVVMTDAGAQVYRHAQMILRQMDQAHA
DVLAAGKHLAGRVSVGLAPFSSAATLSFELLAETRRLYPGIVLHLTESVGQAYSQMLMNNKLEIALIHGTGPMKGVHFDP
LLREDFFLVAHEDFGIEADDKPLSVANLEELPFLLPPPYNFVRRAVDMAFARSRTKLNVIGEVEVIRTLTRAVSGGVGAT
IMPKAVADRITAEASSQIICRTLSPQIEETLSLCVSEHAQLSDSAVAVRDILLRLTRQLKN

Sequences:

>Translated_301_residues
MDIRRFKSFIVIVDTGSITRAADILHIAQPALSQQLASLEEHFGTKLLLRSQQGVVMTDAGAQVYRHAQMILRQMDQAHA
DVLAAGKHLAGRVSVGLAPFSSAATLSFELLAETRRLYPGIVLHLTESVGQAYSQMLMNNKLEIALIHGTGPMKGVHFDP
LLREDFFLVAHEDFGIEADDKPLSVANLEELPFLLPPPYNFVRRAVDMAFARSRTKLNVIGEVEVIRTLTRAVSGGVGAT
IMPKAVADRITAEASSQIICRTLSPQIEETLSLCVSEHAQLSDSAVAVRDILLRLTRQLKN
>Mature_301_residues
MDIRRFKSFIVIVDTGSITRAADILHIAQPALSQQLASLEEHFGTKLLLRSQQGVVMTDAGAQVYRHAQMILRQMDQAHA
DVLAAGKHLAGRVSVGLAPFSSAATLSFELLAETRRLYPGIVLHLTESVGQAYSQMLMNNKLEIALIHGTGPMKGVHFDP
LLREDFFLVAHEDFGIEADDKPLSVANLEELPFLLPPPYNFVRRAVDMAFARSRTKLNVIGEVEVIRTLTRAVSGGVGAT
IMPKAVADRITAEASSQIICRTLSPQIEETLSLCVSEHAQLSDSAVAVRDILLRLTRQLKN

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=293, Percent_Identity=40.6143344709898, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=26.551724137931, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=26.8, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=244, Percent_Identity=24.5901639344262, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=302, Percent_Identity=23.841059602649, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=207, Percent_Identity=26.5700483091787, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787806, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=25.5060728744939, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=25.4437869822485, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=24.5967741935484, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786401, Length=197, Percent_Identity=26.9035532994924, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32985; Mature: 32985

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRRFKSFIVIVDTGSITRAADILHIAQPALSQQLASLEEHFGTKLLLRSQQGVVMTDA
CCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECC
GAQVYRHAQMILRQMDQAHADVLAAGKHLAGRVSVGLAPFSSAATLSFELLAETRRLYPG
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCH
IVLHLTESVGQAYSQMLMNNKLEIALIHGTGPMKGVHFDPLLREDFFLVAHEDFGIEADD
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCCEEEEEECCCCCCCCC
KPLSVANLEELPFLLPPPYNFVRRAVDMAFARSRTKLNVIGEVEVIRTLTRAVSGGVGAT
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCC
IMPKAVADRITAEASSQIICRTLSPQIEETLSLCVSEHAQLSDSAVAVRDILLRLTRQLK
CCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
N
C
>Mature Secondary Structure
MDIRRFKSFIVIVDTGSITRAADILHIAQPALSQQLASLEEHFGTKLLLRSQQGVVMTDA
CCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECC
GAQVYRHAQMILRQMDQAHADVLAAGKHLAGRVSVGLAPFSSAATLSFELLAETRRLYPG
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCH
IVLHLTESVGQAYSQMLMNNKLEIALIHGTGPMKGVHFDPLLREDFFLVAHEDFGIEADD
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCCEEEEEECCCCCCCCC
KPLSVANLEELPFLLPPPYNFVRRAVDMAFARSRTKLNVIGEVEVIRTLTRAVSGGVGAT
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCC
IMPKAVADRITAEASSQIICRTLSPQIEETLSLCVSEHAQLSDSAVAVRDILLRLTRQLK
CCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]