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The map label for this gene is nikB [H]

Identifier: 222102876

GI number: 222102876

Start: 554994

End: 555938

Strand: Direct

Name: nikB [H]

Synonym: Avi_7614

Alternate gene names: 222102876

Gene position: 554994-555938 (Clockwise)

Preceding gene: 222102875

Following gene: 222102877

Centisome position: 87.85

GC content: 58.52

Gene sequence:

>945_bases
ATGGGCGCGTTTATCCTGCGGCGCATTTTGCTGCTGATCCCAATGCTGCTTGGCGCATCACTGGTGATATTTCTCATGCT
GCGGCTTGGTCCGAGCGATCCGGCCATGGATTACTTACGCCTGTCCAAAGTGCCGCCAACGCCTTTGGCGCTGGCAGATG
CCCGGCAGATGCTTGGCCTCGACCGCCCGATTGCCACTCAATATATCGATTGGTTGGGCCATGCCTTCCGGTTGGATTTT
GGCGTATCCTATGCCACGCAAAGGCCGGTTTTGCCAGACTTGCTCTACTATCTTCCGGCAACGCTGCAACTGGCCGGGCT
GGCGCTGATCCTGACCTTTGCCATTTCCATCCCGATGGGTGTCTGGGCATCGCGCCACCGGAACAAGCTGCCCGATCATA
TCGTGCGGCTGGTGTCATTCCTGGGTGTGTCCATGCCCAATTTCTGGCTGGGCTTTCTGCTGGTGCTGGTGTTTTCCGTG
CAGCTCGGCTGGCTTCCCGCCATGGGCCGCGGCGGCTTTGCCCATATGATCATGCCGGCCATTGCCATCGCCTTCATGTC
CCTGTCCATCAATGCGCGGCTGTTGCGCGCCAGCATGCTGGAGGCTGCGGGGCAGCGCCATGTGCTCTATGCCCGGCTGC
GTGGCCTTTCCAGACAGCGAGTTGAGCGGGGCCATATCCTCAGAAACGCGCTTTTGCCAATCATCACCGCCACGGGCATG
CATGTCGGCGAATTGCTTGGCGGCACGCTGGTGATTGAAAGTATTTTCGGCTGGCCCGGTGTTGGCCGCTATGCCGTTTC
TGCCATCCTGAACCGGGACTATCCCGTCATTCAGTGTTTTACCTTGTTGATGGTGGGCATTTTCGTGACCTGTAATCTCC
TTGTCGATATTGCCTATGCATGGGCAGACCCACGGATCAGGCTGTCAGCGGGGCATGTCGAATGA

Upstream 100 bases:

>100_bases
CGCCATTGCCGTTGCCAAAAAGGAGCTTGGCCCCATTCCGTTTGGGGCGATGTCGAGCGAAATTCCGTTTGATCAGATCA
AGCAAAAGACGAACTGATCC

Downstream 100 bases:

>100_bases
GCCAGACGTCAGCATTGCACTCACCCACGCGCCCGGCTGGCTTTTGGCGCTCCTCATCGCTGGGTGTCAAAGCCTGTGGT
GTCATCGTTCTGCTGCTGGT

Product: nickel transporter permease NikB

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MGAFILRRILLLIPMLLGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPLALADARQMLGLDRPIATQYIDWLGHAFRLDF
GVSYATQRPVLPDLLYYLPATLQLAGLALILTFAISIPMGVWASRHRNKLPDHIVRLVSFLGVSMPNFWLGFLLVLVFSV
QLGWLPAMGRGGFAHMIMPAIAIAFMSLSINARLLRASMLEAAGQRHVLYARLRGLSRQRVERGHILRNALLPIITATGM
HVGELLGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLLMVGIFVTCNLLVDIAYAWADPRIRLSAGHVE

Sequences:

>Translated_314_residues
MGAFILRRILLLIPMLLGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPLALADARQMLGLDRPIATQYIDWLGHAFRLDF
GVSYATQRPVLPDLLYYLPATLQLAGLALILTFAISIPMGVWASRHRNKLPDHIVRLVSFLGVSMPNFWLGFLLVLVFSV
QLGWLPAMGRGGFAHMIMPAIAIAFMSLSINARLLRASMLEAAGQRHVLYARLRGLSRQRVERGHILRNALLPIITATGM
HVGELLGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLLMVGIFVTCNLLVDIAYAWADPRIRLSAGHVE
>Mature_313_residues
GAFILRRILLLIPMLLGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPLALADARQMLGLDRPIATQYIDWLGHAFRLDFG
VSYATQRPVLPDLLYYLPATLQLAGLALILTFAISIPMGVWASRHRNKLPDHIVRLVSFLGVSMPNFWLGFLLVLVFSVQ
LGWLPAMGRGGFAHMIMPAIAIAFMSLSINARLLRASMLEAAGQRHVLYARLRGLSRQRVERGHILRNALLPIITATGMH
VGELLGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLLMVGIFVTCNLLVDIAYAWADPRIRLSAGHVE

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=308, Percent_Identity=68.1818181818182, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787053, Length=305, Percent_Identity=37.0491803278689, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1789965, Length=335, Percent_Identity=36.7164179104478, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=33.8709677419355, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787761, Length=335, Percent_Identity=33.7313432835821, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1787550, Length=268, Percent_Identity=32.8358208955224, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788504, Length=363, Percent_Identity=23.6914600550964, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34715; Mature: 34584

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAFILRRILLLIPMLLGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPLALADARQMLGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCC
DRPIATQYIDWLGHAFRLDFGVSYATQRPVLPDLLYYLPATLQLAGLALILTFAISIPMG
CCHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWASRHRNKLPDHIVRLVSFLGVSMPNFWLGFLLVLVFSVQLGWLPAMGRGGFAHMIMPA
HHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IAIAFMSLSINARLLRASMLEAAGQRHVLYARLRGLSRQRVERGHILRNALLPIITATGM
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
HVGELLGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLLMVGIFVTCNLLVDIAYA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WADPRIRLSAGHVE
HCCCCEEECCCCCC
>Mature Secondary Structure 
GAFILRRILLLIPMLLGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPLALADARQMLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCC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HCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]