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The map label for this gene is nitA [H]

Identifier: 222102845

GI number: 222102845

Start: 521926

End: 522969

Strand: Direct

Name: nitA [H]

Synonym: Avi_7576

Alternate gene names: 222102845

Gene position: 521926-522969 (Clockwise)

Preceding gene: 222102844

Following gene: 222102846

Centisome position: 82.61

GC content: 61.02

Gene sequence:

>1044_bases
ATGGAGAAAAGCAGGACCGTTCGGGCTGCCGCCGCACAGATCGCGCCGGATCTCACCTCACGCGACAAAACGCTTGCCCG
CGTGCTGGAAACAATCCGCGAGGCGGCTGGCAAGGGCGCGGAGCTCATCGTCTTTCCAGAAACCTTCGTTCCCTGGTATC
CCTATTTTTCCTTCGTCCTGCCACCGGTTCTGTCTGGCCGCGAACATCTCCGGCTCTACGACGAGGCTGTCACCATTCCG
AGTGTCACGACCGAGGCCGTGGCGGCAGCGGCACGCGAGCACGGCATCGTCGTCGCACTCGGCATCAACGAACGGGATCA
TGGCACGCTTTATAATACACAACTGGTTTTTGATGCGGATGGAGAGCTTGTGCTCAACCGGCGCAAGATCACGCCGACCT
TCCATGAGCGGATGATCTGGGGCCAGGGCGACGCCTCCGGCCTCAAGGTGGTCGATAGCAAAATCGGCCGTATCGGCGCG
CTGGCCTGCTGGGAGCACTACAATCCCCTCGCCCGCTATGCGCTGATGGCCCAGCATGAGGAAATCCACGTCGCGCAATT
CCCCGGCTCCATGGTCGGACCGATCTTTGCCGACCAGATGGAAGTAACGATCCGCCATCATGCGCTGGAAAGCGGCTGTT
TCGTCGTCAACGCCACCGGCTGGCTGACCGACGAGCAGATCCGGTCGATCACCCCTGAGGAAGGCCTGCAAAAGGCACTG
CGCGGCGGCTGCATGACGGCGATCATTTCGCCGGAAGGCAAGCACCTGGCACCCCCGATTACCGAGGGCGAAGGCATCCT
CATTGCCGACCTGGACATGAGCCTGATCCTGAAACGCAAGCGGATGATGGATTCTGTGGGCCACTATGCGCGACCGGAAC
TTCTGCATCTCGTCATCGACGACCGCCCGGCACATCCGATGGTGGCCGCCCATCCTTTCCTCGAAACCGCACCGACAGGG
AGCAATACCGATGGATACCAGACCCCAGCTTTCGACGGAAACGCTGATCAACGAATTGCAGTCGTTCGGCGCGAGGCTCG
TTGA

Upstream 100 bases:

>100_bases
CTGGACACCTGGACGCTCTGACTTATCCCCGCGCTCTGATCAGCGCGCCCGGAAGGCGGGGCCAGCCCCGCCTCCTTCAC
CCCAATCCAAGGAATTCGTC

Downstream 100 bases:

>100_bases
CCCGAAAGCAGGCCACGAAAGCCGTCGCGGTGGTGCAGGCCCTTCCGATCACAAGGCTCTGACCATCGACGGCATGACGG
TGATGGTGCCGGTACACACG

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MEKSRTVRAAAAQIAPDLTSRDKTLARVLETIREAAGKGAELIVFPETFVPWYPYFSFVLPPVLSGREHLRLYDEAVTIP
SVTTEAVAAAAREHGIVVALGINERDHGTLYNTQLVFDADGELVLNRRKITPTFHERMIWGQGDASGLKVVDSKIGRIGA
LACWEHYNPLARYALMAQHEEIHVAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTDEQIRSITPEEGLQKAL
RGGCMTAIISPEGKHLAPPITEGEGILIADLDMSLILKRKRMMDSVGHYARPELLHLVIDDRPAHPMVAAHPFLETAPTG
SNTDGYQTPAFDGNADQRIAVVRREAR

Sequences:

>Translated_347_residues
MEKSRTVRAAAAQIAPDLTSRDKTLARVLETIREAAGKGAELIVFPETFVPWYPYFSFVLPPVLSGREHLRLYDEAVTIP
SVTTEAVAAAAREHGIVVALGINERDHGTLYNTQLVFDADGELVLNRRKITPTFHERMIWGQGDASGLKVVDSKIGRIGA
LACWEHYNPLARYALMAQHEEIHVAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTDEQIRSITPEEGLQKAL
RGGCMTAIISPEGKHLAPPITEGEGILIADLDMSLILKRKRMMDSVGHYARPELLHLVIDDRPAHPMVAAHPFLETAPTG
SNTDGYQTPAFDGNADQRIAVVRREAR
>Mature_347_residues
MEKSRTVRAAAAQIAPDLTSRDKTLARVLETIREAAGKGAELIVFPETFVPWYPYFSFVLPPVLSGREHLRLYDEAVTIP
SVTTEAVAAAAREHGIVVALGINERDHGTLYNTQLVFDADGELVLNRRKITPTFHERMIWGQGDASGLKVVDSKIGRIGA
LACWEHYNPLARYALMAQHEEIHVAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTDEQIRSITPEEGLQKAL
RGGCMTAIISPEGKHLAPPITEGEGILIADLDMSLILKRKRMMDSVGHYARPELLHLVIDDRPAHPMVAAHPFLETAPTG
SNTDGYQTPAFDGNADQRIAVVRREAR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=312, Percent_Identity=32.3717948717949, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322027, Length=190, Percent_Identity=36.8421052631579, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 38209; Mature: 38209

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50007 PIPLC_X_DOMAIN ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSRTVRAAAAQIAPDLTSRDKTLARVLETIREAAGKGAELIVFPETFVPWYPYFSFVL
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
PPVLSGREHLRLYDEAVTIPSVTTEAVAAAAREHGIVVALGINERDHGTLYNTQLVFDAD
CHHHCCHHHHEEHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEECC
GELVLNRRKITPTFHERMIWGQGDASGLKVVDSKIGRIGALACWEHYNPLARYALMAQHE
CCEEEECCCCCHHHHHHEEECCCCCCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHCCC
EIHVAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTDEQIRSITPEEGLQKAL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCHHHHHHHHH
RGGCMTAIISPEGKHLAPPITEGEGILIADLDMSLILKRKRMMDSVGHYARPELLHLVID
CCCCEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEEC
DRPAHPMVAAHPFLETAPTGSNTDGYQTPAFDGNADQRIAVVRREAR
CCCCCCCEEECCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MEKSRTVRAAAAQIAPDLTSRDKTLARVLETIREAAGKGAELIVFPETFVPWYPYFSFVL
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
PPVLSGREHLRLYDEAVTIPSVTTEAVAAAAREHGIVVALGINERDHGTLYNTQLVFDAD
CHHHCCHHHHEEHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEECC
GELVLNRRKITPTFHERMIWGQGDASGLKVVDSKIGRIGALACWEHYNPLARYALMAQHE
CCEEEECCCCCHHHHHHEEECCCCCCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHCCC
EIHVAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTDEQIRSITPEEGLQKAL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCHHHHHHHHH
RGGCMTAIISPEGKHLAPPITEGEGILIADLDMSLILKRKRMMDSVGHYARPELLHLVID
CCCCEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEEC
DRPAHPMVAAHPFLETAPTGSNTDGYQTPAFDGNADQRIAVVRREAR
CCCCCCCEEECCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]