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The map label for this gene is artI [H]

Identifier: 222102794

GI number: 222102794

Start: 457157

End: 457975

Strand: Reverse

Name: artI [H]

Synonym: Avi_7508

Alternate gene names: 222102794

Gene position: 457975-457157 (Counterclockwise)

Preceding gene: 222102795

Following gene: 222102793

Centisome position: 72.49

GC content: 52.87

Gene sequence:

>819_bases
ATGTCCATCACCAAGATCATCCAATCCACTCTGGCATCCACCTTGCTTCTGATTGCCGCCACCAGTGCAGCCTATGCCGA
AGAAGGCGTGTTGCGCATTGGCACCGAAGGGGACGCACCTCTGTTCAGCATGGCTGATGCAAACGGCAACGTCACCGGCT
TTGATGCCGACATTGCCAATGGCATTTGTGCCGAACTGAAGGTCAAGTGCAAATTCGTTGTTCAGGCGTTCAGTTCTCTC
ATTCCATCAATGGACAGTGACCGCTTTGACGTGATCATTTCCGGTCTCGGCATTACGGAAGAACGCGCAAAGAAAATCGA
CTATTCCATTCCCTATGCAACCACACCGCTTTACTTTGTCGTTCCCAAAAGCTCACCTCTTGCCGGGTTGAAGAGCCTTA
CCGAGATTGAAAAGTCATTGACCGGCAAGAGTGTTGGCGTTGTAACCGGCACAACTTACGCAAAATTCATCGAAAAGCGG
ATTCCCGGCGCTGATCTAAAGACCTATGATGCGACCACCCAGCAAACTGCCGATCTGGCCAGTGGCCGCCTTGATGCCGC
CTTCGGCGACTCCCCAACCTGGATGGACTTTCTAGCCAGCGCCGACGGTGCCAATTTTACCAAGATCGATGTCAAGATCA
TGTCCATGGACGATCCTGATGTTCTCGGTCACGGCATGGGCGTTGGCATGCGCAAGGGCAATAGCGCGTTGAAAGCCAAG
GTTGACGCTGCCCTGTGCAAGATGGTGACAGACGGCAAGGTCAAGGACGCCTCGATGAATTGGTTCAAGGACGATTATAC
CATCCCCTGCAAGAAGTGA

Upstream 100 bases:

>100_bases
GCAACCCCGCATCCGGGGCGGTGGAGCGGTTTCTATCCAGCGTCATGCCGGCCGCCGCTTAAGCGGGGTCGTTCCCACAA
CAATAAAAGGGGTAACAGAT

Downstream 100 bases:

>100_bases
TCTCTCTTTTGACACGGCACATTCTGCGCAAAATCAGGATGTGCCGTCGGGGTGGTATGCATGTTGAATGATATTTGGGA
GTTGCTGATTGGCAAAGGGT

Product: ABC transporter substrate binding protein (amino acid)

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSITKIIQSTLASTLLLIAATSAAYAEEGVLRIGTEGDAPLFSMADANGNVTGFDADIANGICAELKVKCKFVVQAFSSL
IPSMDSDRFDVIISGLGITEERAKKIDYSIPYATTPLYFVVPKSSPLAGLKSLTEIEKSLTGKSVGVVTGTTYAKFIEKR
IPGADLKTYDATTQQTADLASGRLDAAFGDSPTWMDFLASADGANFTKIDVKIMSMDDPDVLGHGMGVGMRKGNSALKAK
VDAALCKMVTDGKVKDASMNWFKDDYTIPCKK

Sequences:

>Translated_272_residues
MSITKIIQSTLASTLLLIAATSAAYAEEGVLRIGTEGDAPLFSMADANGNVTGFDADIANGICAELKVKCKFVVQAFSSL
IPSMDSDRFDVIISGLGITEERAKKIDYSIPYATTPLYFVVPKSSPLAGLKSLTEIEKSLTGKSVGVVTGTTYAKFIEKR
IPGADLKTYDATTQQTADLASGRLDAAFGDSPTWMDFLASADGANFTKIDVKIMSMDDPDVLGHGMGVGMRKGNSALKAK
VDAALCKMVTDGKVKDASMNWFKDDYTIPCKK
>Mature_271_residues
SITKIIQSTLASTLLLIAATSAAYAEEGVLRIGTEGDAPLFSMADANGNVTGFDADIANGICAELKVKCKFVVQAFSSLI
PSMDSDRFDVIISGLGITEERAKKIDYSIPYATTPLYFVVPKSSPLAGLKSLTEIEKSLTGKSVGVVTGTTYAKFIEKRI
PGADLKTYDATTQQTADLASGRLDAAFGDSPTWMDFLASADGANFTKIDVKIMSMDDPDVLGHGMGVGMRKGNSALKAKV
DAALCKMVTDGKVKDASMNWFKDDYTIPCKK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=34.0080971659919, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=31.5789473684211, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788649, Length=261, Percent_Identity=34.8659003831418, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788228, Length=247, Percent_Identity=30.7692307692308, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=31.063829787234, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1787031, Length=257, Percent_Identity=26.4591439688716, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28929; Mature: 28798

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITKIIQSTLASTLLLIAATSAAYAEEGVLRIGTEGDAPLFSMADANGNVTGFDADIAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCEEEECHHHHC
GICAELKVKCKFVVQAFSSLIPSMDSDRFDVIISGLGITEERAKKIDYSIPYATTPLYFV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCEEEE
VPKSSPLAGLKSLTEIEKSLTGKSVGVVTGTTYAKFIEKRIPGADLKTYDATTQQTADLA
EECCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCEECCCCHHHHHHHH
SGRLDAAFGDSPTWMDFLASADGANFTKIDVKIMSMDDPDVLGHGMGVGMRKGNSALKAK
CCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHH
VDAALCKMVTDGKVKDASMNWFKDDYTIPCKK
HHHHHHHHHCCCCEECCCCCEECCCEECCCCC
>Mature Secondary Structure 
SITKIIQSTLASTLLLIAATSAAYAEEGVLRIGTEGDAPLFSMADANGNVTGFDADIAN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCEEEECHHHHC
GICAELKVKCKFVVQAFSSLIPSMDSDRFDVIISGLGITEERAKKIDYSIPYATTPLYFV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCEEEE
VPKSSPLAGLKSLTEIEKSLTGKSVGVVTGTTYAKFIEKRIPGADLKTYDATTQQTADLA
EECCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCEECCCCHHHHHHHH
SGRLDAAFGDSPTWMDFLASADGANFTKIDVKIMSMDDPDVLGHGMGVGMRKGNSALKAK
CCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHH
VDAALCKMVTDGKVKDASMNWFKDDYTIPCKK
HHHHHHHHHCCCCEECCCCCEECCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]