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The map label for this gene is degA [H]

Identifier: 222102772

GI number: 222102772

Start: 431762

End: 432781

Strand: Direct

Name: degA [H]

Synonym: Avi_7472

Alternate gene names: 222102772

Gene position: 431762-432781 (Clockwise)

Preceding gene: 222102771

Following gene: 222102773

Centisome position: 68.34

GC content: 58.43

Gene sequence:

>1020_bases
ATGGTCAAGGAACGGGTCACTGGGCTAAAGGATGTTGCCGCCGCCGCTGGCGTGTCGGTGACCACCGTGTCGCGCATGCT
GAATGGCTCGCTCGATCTTCCCGGCGAGACGCGCAAGCGGATTGAAGACGCCATTGTTGCGCTCAAATATCAGCCAAATC
CGCATGCAAGACGTCTCAGCCGTGGCCGCTCGGACACCATCGGTCTTGTCGTGCCGGATATTGCCAATCCGTTCTTTGCA
ACTCTGGTGGCCGCTGTTGAAGAGGAAGCAGATCTGCGCGGTTTGGCGCTATCGCTTTATGCCACGCTAAACCGCCCGGG
CCGCGAAATGGCATATCTCAGTCTGCTGGAGCGCAACCATGTGGATGGCCTGATCTTTATCACCAATCACCCTGACCAGG
GTGAGCTGGCGGCGATCATCAATCGCACTGGAAAAGTGGTTGTGGTCGATGAAGACGTGCCGGATGCGACGGTGCCAAAG
CTGTTTTGTGACAACCGGCAAGGCGGAGAACTGGCCGGGCAACATCTGGCCGGTTTTGGCCATAGCCATGTTCTCTACAT
CGGCGGGCCGGATGTGATGATCAGCACCGAGCGCCGCTTTACCGGCTTTGAAGCCGCCATGCGCGCCAAGCACGGTGAGA
ATGCTGTGATTGAGCGCTATTGCGGCGATTATACCGCCGAGTTTGGCAGACAGGCCGCGCGGCGTTTTCTGGAGGGAGGC
CGCGAGGCGACCGCAATTTTCGCCAGCTCCGACGAAATCGCCATCGGTGTCATTGAAGTGCTGCGAGCGGAAGGGATCTC
CATTCCTGAGCAGATTTCCGTCATCGGCTTCGATGATGTTGGACCTTTGCATCTTTTTGCCCCGCCGTTGACGGCCATCC
GTCAGCCGGTGCGGGGAATGGGAAGCCGTGCTCTTTCGCTGCTTCTCGATACGGATTGGCAACAGCCTGCGAAAGCATCG
GAGGAATTGCTGCCAGTGGAAATCGTCGTGCGGAACTCCGTTGCACCGCCTGCGAAATAA

Upstream 100 bases:

>100_bases
TCGCCGCCGACTAAAAAATAGTCTTCTGGTTGCTTGACAAAAAGCGCAGGGCTATGTGAACTTTGTAAAACGTTTTACTA
AACCGCTTTACAGGGTGGAT

Downstream 100 bases:

>100_bases
CAAAAGCAATGGGCAAACCTAACCAGAGGATGGAATGATGACCCGGACTTTTACACGTAGAACCATCAATGCAGCCTTGG
CTGTCACGGCCCTTACCGGT

Product: transcriptional regulator LacI family

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MVKERVTGLKDVAAAAGVSVTTVSRMLNGSLDLPGETRKRIEDAIVALKYQPNPHARRLSRGRSDTIGLVVPDIANPFFA
TLVAAVEEEADLRGLALSLYATLNRPGREMAYLSLLERNHVDGLIFITNHPDQGELAAIINRTGKVVVVDEDVPDATVPK
LFCDNRQGGELAGQHLAGFGHSHVLYIGGPDVMISTERRFTGFEAAMRAKHGENAVIERYCGDYTAEFGRQAARRFLEGG
REATAIFASSDEIAIGVIEVLRAEGISIPEQISVIGFDDVGPLHLFAPPLTAIRQPVRGMGSRALSLLLDTDWQQPAKAS
EELLPVEIVVRNSVAPPAK

Sequences:

>Translated_339_residues
MVKERVTGLKDVAAAAGVSVTTVSRMLNGSLDLPGETRKRIEDAIVALKYQPNPHARRLSRGRSDTIGLVVPDIANPFFA
TLVAAVEEEADLRGLALSLYATLNRPGREMAYLSLLERNHVDGLIFITNHPDQGELAAIINRTGKVVVVDEDVPDATVPK
LFCDNRQGGELAGQHLAGFGHSHVLYIGGPDVMISTERRFTGFEAAMRAKHGENAVIERYCGDYTAEFGRQAARRFLEGG
REATAIFASSDEIAIGVIEVLRAEGISIPEQISVIGFDDVGPLHLFAPPLTAIRQPVRGMGSRALSLLLDTDWQQPAKAS
EELLPVEIVVRNSVAPPAK
>Mature_339_residues
MVKERVTGLKDVAAAAGVSVTTVSRMLNGSLDLPGETRKRIEDAIVALKYQPNPHARRLSRGRSDTIGLVVPDIANPFFA
TLVAAVEEEADLRGLALSLYATLNRPGREMAYLSLLERNHVDGLIFITNHPDQGELAAIINRTGKVVVVDEDVPDATVPK
LFCDNRQGGELAGQHLAGFGHSHVLYIGGPDVMISTERRFTGFEAAMRAKHGENAVIERYCGDYTAEFGRQAARRFLEGG
REATAIFASSDEIAIGVIEVLRAEGISIPEQISVIGFDDVGPLHLFAPPLTAIRQPVRGMGSRALSLLLDTDWQQPAKAS
EELLPVEIVVRNSVAPPAK

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=30.6547619047619, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787948, Length=311, Percent_Identity=32.475884244373, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=31.9277108433735, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=31.3253012048193, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1790194, Length=305, Percent_Identity=33.4426229508197, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=30.3303303303303, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789068, Length=343, Percent_Identity=28.2798833819242, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786268, Length=307, Percent_Identity=27.3615635179153, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=26.6881028938907, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=26.2820512820513, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787906, Length=320, Percent_Identity=27.8125, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789456, Length=313, Percent_Identity=25.8785942492013, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994940, Length=310, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790192, Length=220, Percent_Identity=30, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36631; Mature: 36631

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKERVTGLKDVAAAAGVSVTTVSRMLNGSLDLPGETRKRIEDAIVALKYQPNPHARRLS
CCCHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHH
RGRSDTIGLVVPDIANPFFATLVAAVEEEADLRGLALSLYATLNRPGREMAYLSLLERNH
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHEEEEHHCCCCCHHHHHHHHHHHCC
VDGLIFITNHPDQGELAAIINRTGKVVVVDEDVPDATVPKLFCDNRQGGELAGQHLAGFG
CCEEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHCCC
HSHVLYIGGPDVMISTERRFTGFEAAMRAKHGENAVIERYCGDYTAEFGRQAARRFLEGG
CCEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCC
REATAIFASSDEIAIGVIEVLRAEGISIPEQISVIGFDDVGPLHLFAPPLTAIRQPVRGM
CEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHH
GSRALSLLLDTDWQQPAKASEELLPVEIVVRNSVAPPAK
HHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MVKERVTGLKDVAAAAGVSVTTVSRMLNGSLDLPGETRKRIEDAIVALKYQPNPHARRLS
CCCHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHH
RGRSDTIGLVVPDIANPFFATLVAAVEEEADLRGLALSLYATLNRPGREMAYLSLLERNH
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHEEEEHHCCCCCHHHHHHHHHHHCC
VDGLIFITNHPDQGELAAIINRTGKVVVVDEDVPDATVPKLFCDNRQGGELAGQHLAGFG
CCEEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHCCC
HSHVLYIGGPDVMISTERRFTGFEAAMRAKHGENAVIERYCGDYTAEFGRQAARRFLEGG
CCEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCC
REATAIFASSDEIAIGVIEVLRAEGISIPEQISVIGFDDVGPLHLFAPPLTAIRQPVRGM
CEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHH
GSRALSLLLDTDWQQPAKASEELLPVEIVVRNSVAPPAK
HHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]