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The map label for this gene is tfdB [H]

Identifier: 222102753

GI number: 222102753

Start: 407029

End: 408786

Strand: Reverse

Name: tfdB [H]

Synonym: Avi_7439

Alternate gene names: 222102753

Gene position: 408786-407029 (Counterclockwise)

Preceding gene: 222102754

Following gene: 222102752

Centisome position: 64.7

GC content: 58.65

Gene sequence:

>1758_bases
ATGGCAGACATTACAACGGATGTGCTGATTATCGGCACTGGACCGGCAGGCTCGGCAGCGGCAGCCCTGCTGTCGACCTA
CGGGATCAACAATATGGTCGTCAACCGCTATCGCTGGTTGGCCAATTCACCACGCGCTCACATTACCAACCAGCGTGCCA
TGGAAGTGCTGCGCGATCTTGGCCGTGATGTGGAGGACGAGGCCTACTTGTTTGCGACGCATCAGGAACTGATGGGCGAA
AACATTTTCTGCGAAAGTCTGGCGGGTGAAGAAATTGGCCGCCTGAAAGCTTGGGGCAATCATCCCCTGAGCAAAGCTGA
ACATCTGATGTCATCTCCGACGAAGATGAATGACTTGCCGCAAACTTTCATGGAGCCTTTGCTGTTCAAGGCAGCCTGCT
CTCGCGGCACTCAAGCGCGCATGTCCACCGAATATCTGCGCCACGAACAAAATGCCGAAGGCGTGTTGACCACCTGCAAG
GATCGGCTTTCGGGACATGAACTGACCATCCAGTCGAAATATCTGATCGGTGCCGATGGCGCAAAGTCGCTGGTGGCCGA
GCATGCTGACCTGCCGTTTGAAGGCAAAATGGGTGTCGGCGGCTCGATGAACATCCTGTTCAAGGCCGACCTCACCAAAT
ATGTCGCGCATCGTCCTTCGGTTCTCTACTGGGTCATGCAGCCGGGTGCCGATGTCGGCGGCATTGGCATGGGGCTGGTG
CGCATGGTGCGCCCTTGGAATGAATGGCTCATCGTCTGGGGCTATGACATCAACGGGCCCGAACCGGAAGTGACAGAGGA
GCTTGCCACCGGCGTGGCGCGGCAGCTGATCGGCGATCCTGATTTGGAAATTGAACTGTTGGCGGCCAACACCTGGACCG
TGAACAATTATTATGCCACCCGCACCTCCAATGGCCGCGTGTTCTGCGTTGGCGATGCCATCCACAGGCATCCGCCCTCC
AATGGCCTCGGCTCAAACACCTCGATTCAGGATTCCTTCAACCTTGCCTGGAAGCTTGCCATGGTTCTGAAGGGTCAGGC
TGGCGAGGGCCTGCTGGAAAGCTTCAACGTCGAACGCGCCCCAATCGCCAAGCAGATCGTCAGCCGCGCCAACCAGTCGA
TTGGCGAAACCGGGCCGATTTTCGCAGCACTTGGCATGAGCGAAGGCGTCGATCCAGAGCAGATGCAGAAAAATCTTGAA
GCCCGCACGGATGGAACCGAGGCTGCCGAACAGCAACGCGAGGCCATCCGCAAGGCAATCGCCTTCAAGAAATATGAGTT
TGACGCGCATGGCATCGAAATGAACCAGCGTTATCGGTCCGATGCCATCGTCACCGACGGGCAGATCGAACCCGACTTCC
TGCTGGATGCCGACCTGCATCACCAGCCAACCACTTGGCCGGGTGCACGCCTACCGCATGCCTGGCTCTACAAACATGTC
GGAGGTGCCGAAGTATCAACTCTCGATCTCTGCGGCCATGGCAAATTCACGCTGCTGACGGGCCTGGGTGGAAAAGCCTG
GATTGACGCCGCCGCAGCGGTGGGCAGCGAACTGGGCATGGAGATCAACGCGCATATGATCGGCCCGCGCCAAACCTATG
TCGATCCCACCGGCGACTGGGCGCGGTTGAGCGAGATCCGCGACACCGGCTGCGTTCTTGTTCGGCCAGACCACCATGTG
TGCTGGCGCAGCACGGCAGCAAGCGGCACACCGGCGGACGACTTGCGCCGTGTGCTGCGGCAGATTTTGGCGCTCTAA

Upstream 100 bases:

>100_bases
CGTCCTGTGGAGCGGGACGCGATAAGGCGGCTTTTGCAAGCGGCATGGACCGGAGAGCCGCCGGTCGCCTGACAGAGTTG
AAGAGGGAGGAAATTGACAA

Downstream 100 bases:

>100_bases
GCCAGCAATGGCACCGAGATGATCGGTGCCATTCGAATGCCAGTTCATAACCTGATGACATATTTACCACGACGATTCAG
ATCAATTATGTGGTTTCTGG

Product: 2,4-dichlorophenol hydroxylase

Products: NA

Alternate protein names: 2,4-dichlorophenol hydroxylase; 2,4-DCP hydroxylase [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MADITTDVLIIGTGPAGSAAAALLSTYGINNMVVNRYRWLANSPRAHITNQRAMEVLRDLGRDVEDEAYLFATHQELMGE
NIFCESLAGEEIGRLKAWGNHPLSKAEHLMSSPTKMNDLPQTFMEPLLFKAACSRGTQARMSTEYLRHEQNAEGVLTTCK
DRLSGHELTIQSKYLIGADGAKSLVAEHADLPFEGKMGVGGSMNILFKADLTKYVAHRPSVLYWVMQPGADVGGIGMGLV
RMVRPWNEWLIVWGYDINGPEPEVTEELATGVARQLIGDPDLEIELLAANTWTVNNYYATRTSNGRVFCVGDAIHRHPPS
NGLGSNTSIQDSFNLAWKLAMVLKGQAGEGLLESFNVERAPIAKQIVSRANQSIGETGPIFAALGMSEGVDPEQMQKNLE
ARTDGTEAAEQQREAIRKAIAFKKYEFDAHGIEMNQRYRSDAIVTDGQIEPDFLLDADLHHQPTTWPGARLPHAWLYKHV
GGAEVSTLDLCGHGKFTLLTGLGGKAWIDAAAAVGSELGMEINAHMIGPRQTYVDPTGDWARLSEIRDTGCVLVRPDHHV
CWRSTAASGTPADDLRRVLRQILAL

Sequences:

>Translated_585_residues
MADITTDVLIIGTGPAGSAAAALLSTYGINNMVVNRYRWLANSPRAHITNQRAMEVLRDLGRDVEDEAYLFATHQELMGE
NIFCESLAGEEIGRLKAWGNHPLSKAEHLMSSPTKMNDLPQTFMEPLLFKAACSRGTQARMSTEYLRHEQNAEGVLTTCK
DRLSGHELTIQSKYLIGADGAKSLVAEHADLPFEGKMGVGGSMNILFKADLTKYVAHRPSVLYWVMQPGADVGGIGMGLV
RMVRPWNEWLIVWGYDINGPEPEVTEELATGVARQLIGDPDLEIELLAANTWTVNNYYATRTSNGRVFCVGDAIHRHPPS
NGLGSNTSIQDSFNLAWKLAMVLKGQAGEGLLESFNVERAPIAKQIVSRANQSIGETGPIFAALGMSEGVDPEQMQKNLE
ARTDGTEAAEQQREAIRKAIAFKKYEFDAHGIEMNQRYRSDAIVTDGQIEPDFLLDADLHHQPTTWPGARLPHAWLYKHV
GGAEVSTLDLCGHGKFTLLTGLGGKAWIDAAAAVGSELGMEINAHMIGPRQTYVDPTGDWARLSEIRDTGCVLVRPDHHV
CWRSTAASGTPADDLRRVLRQILAL
>Mature_584_residues
ADITTDVLIIGTGPAGSAAAALLSTYGINNMVVNRYRWLANSPRAHITNQRAMEVLRDLGRDVEDEAYLFATHQELMGEN
IFCESLAGEEIGRLKAWGNHPLSKAEHLMSSPTKMNDLPQTFMEPLLFKAACSRGTQARMSTEYLRHEQNAEGVLTTCKD
RLSGHELTIQSKYLIGADGAKSLVAEHADLPFEGKMGVGGSMNILFKADLTKYVAHRPSVLYWVMQPGADVGGIGMGLVR
MVRPWNEWLIVWGYDINGPEPEVTEELATGVARQLIGDPDLEIELLAANTWTVNNYYATRTSNGRVFCVGDAIHRHPPSN
GLGSNTSIQDSFNLAWKLAMVLKGQAGEGLLESFNVERAPIAKQIVSRANQSIGETGPIFAALGMSEGVDPEQMQKNLEA
RTDGTEAAEQQREAIRKAIAFKKYEFDAHGIEMNQRYRSDAIVTDGQIEPDFLLDADLHHQPTTWPGARLPHAWLYKHVG
GAEVSTLDLCGHGKFTLLTGLGGKAWIDAAAAVGSELGMEINAHMIGPRQTYVDPTGDWARLSEIRDTGCVLVRPDHHVC
WRSTAASGTPADDLRRVLRQILAL

Specific function: Transforms 2,4-dichlorophenol (2,4-DCP) into 3,5- dichlorocatechol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=365, Percent_Identity=26.3013698630137, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.20 [H]

Molecular weight: Translated: 64118; Mature: 63987

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADITTDVLIIGTGPAGSAAAALLSTYGINNMVVNRYRWLANSPRAHITNQRAMEVLRDL
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHH
GRDVEDEAYLFATHQELMGENIFCESLAGEEIGRLKAWGNHPLSKAEHLMSSPTKMNDLP
CCCCCCCEEEEEEHHHHCCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCH
QTFMEPLLFKAACSRGTQARMSTEYLRHEQNAEGVLTTCKDRLSGHELTIQSKYLIGADG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCC
AKSLVAEHADLPFEGKMGVGGSMNILFKADLTKYVAHRPSVLYWVMQPGADVGGIGMGLV
HHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RMVRPWNEWLIVWGYDINGPEPEVTEELATGVARQLIGDPDLEIELLAANTWTVNNYYAT
HHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECEEEECCEEEE
RTSNGRVFCVGDAIHRHPPSNGLGSNTSIQDSFNLAWKLAMVLKGQAGEGLLESFNVERA
ECCCCEEEEEECHHHCCCCCCCCCCCCCCCCCHHHHHEEEEHHCCCCCCHHHHHCCCCCC
PIAKQIVSRANQSIGETGPIFAALGMSEGVDPEQMQKNLEARTDGTEAAEQQREAIRKAI
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AFKKYEFDAHGIEMNQRYRSDAIVTDGQIEPDFLLDADLHHQPTTWPGARLPHAWLYKHV
HHHHEECCCCCCCHHHHHCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHC
GGAEVSTLDLCGHGKFTLLTGLGGKAWIDAAAAVGSELGMEINAHMIGPRQTYVDPTGDW
CCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCH
ARLSEIRDTGCVLVRPDHHVCWRSTAASGTPADDLRRVLRQILAL
HHHHHHCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADITTDVLIIGTGPAGSAAAALLSTYGINNMVVNRYRWLANSPRAHITNQRAMEVLRDL
CCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHH
GRDVEDEAYLFATHQELMGENIFCESLAGEEIGRLKAWGNHPLSKAEHLMSSPTKMNDLP
CCCCCCCEEEEEEHHHHCCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCH
QTFMEPLLFKAACSRGTQARMSTEYLRHEQNAEGVLTTCKDRLSGHELTIQSKYLIGADG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCC
AKSLVAEHADLPFEGKMGVGGSMNILFKADLTKYVAHRPSVLYWVMQPGADVGGIGMGLV
HHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RMVRPWNEWLIVWGYDINGPEPEVTEELATGVARQLIGDPDLEIELLAANTWTVNNYYAT
HHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECEEEECCEEEE
RTSNGRVFCVGDAIHRHPPSNGLGSNTSIQDSFNLAWKLAMVLKGQAGEGLLESFNVERA
ECCCCEEEEEECHHHCCCCCCCCCCCCCCCCCHHHHHEEEEHHCCCCCCHHHHHCCCCCC
PIAKQIVSRANQSIGETGPIFAALGMSEGVDPEQMQKNLEARTDGTEAAEQQREAIRKAI
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AFKKYEFDAHGIEMNQRYRSDAIVTDGQIEPDFLLDADLHHQPTTWPGARLPHAWLYKHV
HHHHEECCCCCCCHHHHHCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHC
GGAEVSTLDLCGHGKFTLLTGLGGKAWIDAAAAVGSELGMEINAHMIGPRQTYVDPTGDW
CCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCH
ARLSEIRDTGCVLVRPDHHVCWRSTAASGTPADDLRRVLRQILAL
HHHHHHCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2185214 [H]