BacMap

Definition	Agrobacterium vitis S4 plasmid pAtS4e, complete sequence.
Accession	NC_011981
Length	631,775

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The map label for this gene is livF [H]

Identifier: 222102750

GI number: 222102750

Start: 403913

End: 404638

Strand: Reverse

Name: livF [H]

Synonym: Avi_7436

Alternate gene names: 222102750

Gene position: 404638-403913 (Counterclockwise)

Preceding gene: 222102751

Following gene: 222102749

Centisome position: 64.05

GC content: 58.95

Gene sequence:

>726_bases
ATGATGACCAGTCTGCTCGAAACACGCGGCCTCACGGCATTCTACGGCGACTTTCAGGCTCTGTTCGGCGTCGATCTCAG
GCTGGACGCCGGAGAAATGATTGCGGTGATTGGGGCCAATGGCGCTGGAAAGTCCACCTTGATGCGTGCCATTGTCGGGG
TGCTGGCGTCTGAGCCAGACGCCATCCTGCACCAAGGCGTGTCCATCGGTGCATTGGCGGCACCCGATGTGATGGCCCGT
GGCATTGCGCTGGTGCCGGAAGGGCGCAAGCTTTTCCCCTCGCTCACCGTCGAGGAAAATTTGCTGATCGGCCAATATGG
CCGCACGGTCAATGGCCCATGGTCGCTTGCCAGTATTTACGAGCTTTTCCCGATATTGCAGGAACGCCGCCACAGACCTG
CGACAGCGCTTTCCGGTGGTCAACAGCAAATGGTTGCGATTGGCCGGGCGCTGATGTCCAATCCCGAGGTCCTGTTGTGC
GATGAGCTGAGCCTTGGCCTTGCCCCGGTGGTGATCAAGGATATCTATGCCGCATTCCCACGCATCCGCGCCGCAGGTGC
TGCTATCATCATTGTCGAGCAAGATATTGGTCAGGCGCTGAAACAGGCAGATCGCGTCTATTGCATGATGGAGGGCCGCA
TCACACTGACGGGCCGCCCGTCCGATCTCGATCGCAACGACATTCATGCCGCGTATTTCGGAGCCGAATCCCATGAACTG
GCTTGA

Upstream 100 bases:

>100_bases
GCATCCTTGTGCTGAACTTCGGCCGGGTGCTGGGCATTGGCGAACCGCAGGCGATCATGGCCTCGCGAGAGGTTCGCGAA
ATCTATCTGGGTCTGGAGGT

Downstream 100 bases:

>100_bases
TACCATTCTGCAAGGCGCGCTGCTGGGCGGCCTCTACGCGCTGTTTGCCGCAGGTCTCAGTCTGGTCTTCGGCATTATGC
GACTGGTCAATCTTGCGCAT

Product: ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MMTSLLETRGLTAFYGDFQALFGVDLRLDAGEMIAVIGANGAGKSTLMRAIVGVLASEPDAILHQGVSIGALAAPDVMAR
GIALVPEGRKLFPSLTVEENLLIGQYGRTVNGPWSLASIYELFPILQERRHRPATALSGGQQQMVAIGRALMSNPEVLLC
DELSLGLAPVVIKDIYAAFPRIRAAGAAIIIVEQDIGQALKQADRVYCMMEGRITLTGRPSDLDRNDIHAAYFGAESHEL
A

Sequences:

>Translated_241_residues
MMTSLLETRGLTAFYGDFQALFGVDLRLDAGEMIAVIGANGAGKSTLMRAIVGVLASEPDAILHQGVSIGALAAPDVMAR
GIALVPEGRKLFPSLTVEENLLIGQYGRTVNGPWSLASIYELFPILQERRHRPATALSGGQQQMVAIGRALMSNPEVLLC
DELSLGLAPVVIKDIYAAFPRIRAAGAAIIIVEQDIGQALKQADRVYCMMEGRITLTGRPSDLDRNDIHAAYFGAESHEL
A
>Mature_241_residues
MMTSLLETRGLTAFYGDFQALFGVDLRLDAGEMIAVIGANGAGKSTLMRAIVGVLASEPDAILHQGVSIGALAAPDVMAR
GIALVPEGRKLFPSLTVEENLLIGQYGRTVNGPWSLASIYELFPILQERRHRPATALSGGQQQMVAIGRALMSNPEVLLC
DELSLGLAPVVIKDIYAAFPRIRAAGAAIIIVEQDIGQALKQADRVYCMMEGRITLTGRPSDLDRNDIHAAYFGAESHEL
A

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI148612844, Length=245, Percent_Identity=28.1632653061224, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI255708477, Length=245, Percent_Identity=28.1632653061224, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI27881501, Length=217, Percent_Identity=25.3456221198157, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI30795238, Length=217, Percent_Identity=25.3456221198157, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI153792144, Length=215, Percent_Identity=26.9767441860465, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI42490749, Length=221, Percent_Identity=25.7918552036199, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI9961252, Length=224, Percent_Identity=27.2321428571429, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI4505771, Length=224, Percent_Identity=27.2321428571429, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI9961250, Length=224, Percent_Identity=27.2321428571429, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI105990541, Length=202, Percent_Identity=24.7524752475248, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI27436953, Length=212, Percent_Identity=25, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87082268, Length=236, Percent_Identity=41.9491525423729, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1787792, Length=226, Percent_Identity=32.3008849557522, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787029, Length=226, Percent_Identity=32.3008849557522, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1789864, Length=254, Percent_Identity=27.5590551181102, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081791, Length=228, Percent_Identity=30.2631578947368, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787089, Length=227, Percent_Identity=32.15859030837, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790544, Length=253, Percent_Identity=27.2727272727273, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787712, Length=227, Percent_Identity=30.8370044052863, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789593, Length=235, Percent_Identity=25.9574468085106, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789672, Length=225, Percent_Identity=27.5555555555556, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790190, Length=204, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787370, Length=225, Percent_Identity=28, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48995001, Length=218, Percent_Identity=28.8990825688073, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788450, Length=243, Percent_Identity=31.6872427983539, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790467, Length=227, Percent_Identity=29.5154185022026, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788472, Length=215, Percent_Identity=26.046511627907, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788225, Length=235, Percent_Identity=30.2127659574468, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790525, Length=245, Percent_Identity=26.9387755102041, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789991, Length=247, Percent_Identity=28.3400809716599, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786872, Length=222, Percent_Identity=29.2792792792793, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788897, Length=203, Percent_Identity=29.5566502463054, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786398, Length=202, Percent_Identity=31.1881188118812, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787105, Length=224, Percent_Identity=33.9285714285714, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788761, Length=231, Percent_Identity=29.4372294372294, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1789032, Length=229, Percent_Identity=26.2008733624454, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87081709, Length=226, Percent_Identity=26.9911504424779, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790739, Length=244, Percent_Identity=27.8688524590164, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1789873, Length=209, Percent_Identity=29.6650717703349, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI87081782, Length=225, Percent_Identity=25.3333333333333, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788210, Length=225, Percent_Identity=27.1111111111111, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790162, Length=234, Percent_Identity=26.4957264957265, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081834, Length=227, Percent_Identity=29.5154185022026, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787164, Length=211, Percent_Identity=29.8578199052133, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786319, Length=203, Percent_Identity=30.0492610837438, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI48994943, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788165, Length=246, Percent_Identity=26.0162601626016, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082267, Length=217, Percent_Identity=29.4930875576037, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786563, Length=211, Percent_Identity=28.436018957346, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788644, Length=239, Percent_Identity=29.2887029288703, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786698, Length=209, Percent_Identity=24.8803827751196, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786703, Length=216, Percent_Identity=30.5555555555556, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787041, Length=162, Percent_Identity=28.3950617283951, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786253, Length=233, Percent_Identity=25.3218884120172, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193209708, Length=226, Percent_Identity=31.4159292035398, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193211017, Length=222, Percent_Identity=29.7297297297297, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193211015, Length=222, Percent_Identity=29.7297297297297, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193202349, Length=214, Percent_Identity=27.5700934579439, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71996809, Length=222, Percent_Identity=29.7297297297297, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71984940, Length=216, Percent_Identity=28.7037037037037, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146777, Length=244, Percent_Identity=27.8688524590164, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17541710, Length=216, Percent_Identity=28.7037037037037, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17565938, Length=210, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=205, Percent_Identity=27.3170731707317, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=205, Percent_Identity=27.3170731707317, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=205, Percent_Identity=27.3170731707317, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=152, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=212, Percent_Identity=25, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71996550, Length=214, Percent_Identity=25.2336448598131, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=218, Percent_Identity=26.1467889908257, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24666092, Length=216, Percent_Identity=25.462962962963, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357907, Length=208, Percent_Identity=29.8076923076923, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221512771, Length=207, Percent_Identity=24.6376811594203, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650853, Length=206, Percent_Identity=25.7281553398058, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24650855, Length=206, Percent_Identity=25.7281553398058, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24666836, Length=177, Percent_Identity=32.7683615819209, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281362751, Length=206, Percent_Identity=25.7281553398058, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28574533, Length=215, Percent_Identity=26.9767441860465, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24643674, Length=234, Percent_Identity=24.7863247863248, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85724864, Length=207, Percent_Identity=25.6038647342995, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580555, Length=207, Percent_Identity=25.6038647342995, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85816269, Length=207, Percent_Identity=25.6038647342995, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580553, Length=207, Percent_Identity=25.6038647342995, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920532, Length=213, Percent_Identity=26.2910798122066, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580930, Length=213, Percent_Identity=26.2910798122066, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136662, Length=208, Percent_Identity=27.4038461538462, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI62484234, Length=221, Percent_Identity=28.0542986425339, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25787; Mature: 25787

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTSLLETRGLTAFYGDFQALFGVDLRLDAGEMIAVIGANGAGKSTLMRAIVGVLASEPD
CCCHHHHHCCCEEEHHHHHHHHCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCH
AILHQGVSIGALAAPDVMARGIALVPEGRKLFPSLTVEENLLIGQYGRTVNGPWSLASIY
HHHHCCCCCCCCCCCHHHHCCEEECCCCCHHCCCCCCCCCEEEECCCCCCCCCHHHHHHH
ELFPILQERRHRPATALSGGQQQMVAIGRALMSNPEVLLCDELSLGLAPVVIKDIYAAFP
HHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
RIRAAGAAIIIVEQDIGQALKQADRVYCMMEGRITLTGRPSDLDRNDIHAAYFGAESHEL
HHHHCCEEEEEEEHHHHHHHHHHCCEEEEEECEEEEECCCCCCCCCCCEEEEECCCCCCC
A
C
>Mature Secondary Structure
MMTSLLETRGLTAFYGDFQALFGVDLRLDAGEMIAVIGANGAGKSTLMRAIVGVLASEPD
CCCHHHHHCCCEEEHHHHHHHHCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCH
AILHQGVSIGALAAPDVMARGIALVPEGRKLFPSLTVEENLLIGQYGRTVNGPWSLASIY
HHHHCCCCCCCCCCCHHHHCCEEECCCCCHHCCCCCCCCCEEEECCCCCCCCCHHHHHHH
ELFPILQERRHRPATALSGGQQQMVAIGRALMSNPEVLLCDELSLGLAPVVIKDIYAAFP
HHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
RIRAAGAAIIIVEQDIGQALKQADRVYCMMEGRITLTGRPSDLDRNDIHAAYFGAESHEL
HHHHCCEEEEEEEHHHHHHHHHHCCEEEEEECEEEEECCCCCCCCCCCEEEEECCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]