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The map label for this gene is livM [H]

Identifier: 222102748

GI number: 222102748

Start: 401961

End: 403052

Strand: Reverse

Name: livM [H]

Synonym: Avi_7433

Alternate gene names: 222102748

Gene position: 403052-401961 (Counterclockwise)

Preceding gene: 222102749

Following gene: 222102742

Centisome position: 63.8

GC content: 54.58

Gene sequence:

>1092_bases
ATGACATCCACCAACATCGTCCCAACCCAAGAGAATCCACCAACCGGCTGGCGCATTCAAACACGAACACGGCTATCTTC
AGCCTTTACGATAGGACTCATACTGGTCTTTGCAGTGCTGGCCTTCGCTCCGTTTTTCGTGTCGCGTGGCACAGTGCAAA
ATCTGTTTTACATCCTGACGATGCTGACATTGGCACAGAGCTGGAACCTGCTTGCCGGATATGCCGGATTGATTTCGGTC
GGCCAACAACTGTTTGTCGGTTGTGGGGCCTATGCGCTTTTTGCAATGGTCATTCTGCTTGGCGTCAATCCGCTGATTGC
CATTCCGCTGGCGGGCGTCTTTGCCACGATCGTGGCACTGCCCACCGCCCTCTTCACCTTCCGTCTCCACGGCTCCTATT
TCGCCATCGGCACCTGGGTGATTGCAGAAGTCGGGCGGCTGGTGTTTGCCCAATGGAAAACGCTGGGCGGCGGCACCGGG
GCGTCGCTGCCACGCGAGGCGACCCGCGAGATGTTTGGCGTTTCCACCATCAGCGCATTGTTCGGCATGAAACCGGCTGT
TGCGCTGGATTCACTGGCCTATTGGCTCGCATTGTTGTTGACGGGTTTCACCATCGTTTTCGTCTATCGGCTGTTGCGCA
GCAAGCAAGGACTGGGCCTTGCTGCTGTGCGCGACAATGAAACAGCGGCAACGGCACTCGGCGTGGATGCCCTTCGTCTG
AAATTGACGATTTATCTTCTCACAGCAGCCCTGACCGGCATGACTGGCGCACTGATCTATCTTCAGAAAGCCCGAATTTC
ACCCGATGCCGCCTTTTCACTCACAGATTGGACAGCCTATGTACTGTTTATCGTCGTGGTGGGTGGAATCGGCACCATCG
AAGGGCCCATCGTTGGCGTGATCATTTTGTTTTTCCTGCAAAATCTTCTGTCAGATTTTGGCTCATGGTATCTGTTATTG
CTGGGCGCACTGGCAATTTTAACAATGCTGTTTGCACCACGCGGTATCTGGGGCCTGTTCTCAGAGCGCACCGGCATTGA
GTTTTTCCCGGTTCGACGGCTGTTGCGCGGTAGAACGAAAAACAAAAACTGA

Upstream 100 bases:

>100_bases
CCCGAATGGCAGATTTTGGCAGGCCATATCGCCTTCTTGATCGTGCTTCTGGTCAGGCCACGCGGCTTGTTTCCCCGCGC
CAATGATTGAGGATCAGCAG

Downstream 100 bases:

>100_bases
CCTCACCGACAAGCAGTGCAAGGATCAGCTCACCCCCAAATCCCCAGAGCCCTCTTCTACCCCGATCTCAAAGGCATTGA
GGGTCATAGCAACAAACGTG

Product: ABC transporter membrane spanning protein (branched chain amino acid)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTSTNIVPTQENPPTGWRIQTRTRLSSAFTIGLILVFAVLAFAPFFVSRGTVQNLFYILTMLTLAQSWNLLAGYAGLISV
GQQLFVGCGAYALFAMVILLGVNPLIAIPLAGVFATIVALPTALFTFRLHGSYFAIGTWVIAEVGRLVFAQWKTLGGGTG
ASLPREATREMFGVSTISALFGMKPAVALDSLAYWLALLLTGFTIVFVYRLLRSKQGLGLAAVRDNETAATALGVDALRL
KLTIYLLTAALTGMTGALIYLQKARISPDAAFSLTDWTAYVLFIVVVGGIGTIEGPIVGVIILFFLQNLLSDFGSWYLLL
LGALAILTMLFAPRGIWGLFSERTGIEFFPVRRLLRGRTKNKN

Sequences:

>Translated_363_residues
MTSTNIVPTQENPPTGWRIQTRTRLSSAFTIGLILVFAVLAFAPFFVSRGTVQNLFYILTMLTLAQSWNLLAGYAGLISV
GQQLFVGCGAYALFAMVILLGVNPLIAIPLAGVFATIVALPTALFTFRLHGSYFAIGTWVIAEVGRLVFAQWKTLGGGTG
ASLPREATREMFGVSTISALFGMKPAVALDSLAYWLALLLTGFTIVFVYRLLRSKQGLGLAAVRDNETAATALGVDALRL
KLTIYLLTAALTGMTGALIYLQKARISPDAAFSLTDWTAYVLFIVVVGGIGTIEGPIVGVIILFFLQNLLSDFGSWYLLL
LGALAILTMLFAPRGIWGLFSERTGIEFFPVRRLLRGRTKNKN
>Mature_362_residues
TSTNIVPTQENPPTGWRIQTRTRLSSAFTIGLILVFAVLAFAPFFVSRGTVQNLFYILTMLTLAQSWNLLAGYAGLISVG
QQLFVGCGAYALFAMVILLGVNPLIAIPLAGVFATIVALPTALFTFRLHGSYFAIGTWVIAEVGRLVFAQWKTLGGGTGA
SLPREATREMFGVSTISALFGMKPAVALDSLAYWLALLLTGFTIVFVYRLLRSKQGLGLAAVRDNETAATALGVDALRLK
LTIYLLTAALTGMTGALIYLQKARISPDAAFSLTDWTAYVLFIVVVGGIGTIEGPIVGVIILFFLQNLLSDFGSWYLLLL
GALAILTMLFAPRGIWGLFSERTGIEFFPVRRLLRGRTKNKN

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=305, Percent_Identity=30.4918032786885, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39263; Mature: 39132

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTNIVPTQENPPTGWRIQTRTRLSSAFTIGLILVFAVLAFAPFFVSRGTVQNLFYILT
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MLTLAQSWNLLAGYAGLISVGQQLFVGCGAYALFAMVILLGVNPLIAIPLAGVFATIVAL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
PTALFTFRLHGSYFAIGTWVIAEVGRLVFAQWKTLGGGTGASLPREATREMFGVSTISAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FGMKPAVALDSLAYWLALLLTGFTIVFVYRLLRSKQGLGLAAVRDNETAATALGVDALRL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
KLTIYLLTAALTGMTGALIYLQKARISPDAAFSLTDWTAYVLFIVVVGGIGTIEGPIVGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
IILFFLQNLLSDFGSWYLLLLGALAILTMLFAPRGIWGLFSERTGIEFFPVRRLLRGRTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCC
NKN
CCC
>Mature Secondary Structure 
TSTNIVPTQENPPTGWRIQTRTRLSSAFTIGLILVFAVLAFAPFFVSRGTVQNLFYILT
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MLTLAQSWNLLAGYAGLISVGQQLFVGCGAYALFAMVILLGVNPLIAIPLAGVFATIVAL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
PTALFTFRLHGSYFAIGTWVIAEVGRLVFAQWKTLGGGTGASLPREATREMFGVSTISAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FGMKPAVALDSLAYWLALLLTGFTIVFVYRLLRSKQGLGLAAVRDNETAATALGVDALRL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
KLTIYLLTAALTGMTGALIYLQKARISPDAAFSLTDWTAYVLFIVVVGGIGTIEGPIVGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
IILFFLQNLLSDFGSWYLLLLGALAILTMLFAPRGIWGLFSERTGIEFFPVRRLLRGRTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCC
NKN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]