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The map label for this gene is rutF [H]

Identifier: 222102727

GI number: 222102727

Start: 378983

End: 379618

Strand: Direct

Name: rutF [H]

Synonym: Avi_7406

Alternate gene names: 222102727

Gene position: 378983-379618 (Clockwise)

Preceding gene: 222102726

Following gene: 222102728

Centisome position: 59.99

GC content: 54.56

Gene sequence:

>636_bases
ATGCAACATCACGTTCACACCAACAATGATAAGGTTGCAGCGGACACCAAGAGTGTTCTTCCTGCAACGGACTGGACATC
AGAACCACAGCAGATAGAGACCTCGGCGGTAACAAGCGGGGGCGGGCTGACCGCAGCCGAGGAAAAAGCCGCTCTGAAAG
CGAAATTCATCTCTGGCATGGGGCGGGTTGCGGCTCCCGTTTCTATTGTTGCCACCGATGGCCCGGCAGGGCGTGCGGGC
GTCACAATCTCAGCAATGAGTTCCGTCTCTGCCGATGGAGACCGACCGACCCTGATGATCTGCCTCAACCAGTCCTCACG
TACATGTCAGGCCCTCGAGAAAAACCGCCATTTCACTCTCAATGTTCTGAATGCACGCAAGCATATCCCCATAGCCGATG
CCTTTGCAGGCCGTTTCGGGACGTTTTTGCCCGATAAATTCGCGATGGGCGACTGGAAGACCAGCCGATTCGGGATTCCA
GAGTTGAATGAGGCGACAGCGGTGTTCCATTGCCAAGTCAACTTCACCCAGATTGTTGGCACACATATGCTCATTATCGC
AGAAGTTGACGAGGTCAGTGAAGCTGAAGGAGCACGGGCTCTCGTTTACTGTAGCCGTGAATATCGTTGCCCTTGA

Upstream 100 bases:

>100_bases
AGTTGGCTGCGCGGAGATTTCCAGAAGGACCGCAAGGCCCCCGCCCGTAAGCAACCGGAATTGGAAAACAGGCGCACGCG
AAACTCAACGGGAGGTGGGT

Downstream 100 bases:

>100_bases
GCGGCTTGCGGGAGGGAGCAAGCCGCAAAATTCAAAGGGAATAATCGTGCAATTTTGATCGCTTTCACACTCTGAAAGCC
GTCACCGATCAGGGCGCGAC

Product: 4-hydroxyphenylacetate 3-monooxygenase reductase component

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MQHHVHTNNDKVAADTKSVLPATDWTSEPQQIETSAVTSGGGLTAAEEKAALKAKFISGMGRVAAPVSIVATDGPAGRAG
VTISAMSSVSADGDRPTLMICLNQSSRTCQALEKNRHFTLNVLNARKHIPIADAFAGRFGTFLPDKFAMGDWKTSRFGIP
ELNEATAVFHCQVNFTQIVGTHMLIIAEVDEVSEAEGARALVYCSREYRCP

Sequences:

>Translated_211_residues
MQHHVHTNNDKVAADTKSVLPATDWTSEPQQIETSAVTSGGGLTAAEEKAALKAKFISGMGRVAAPVSIVATDGPAGRAG
VTISAMSSVSADGDRPTLMICLNQSSRTCQALEKNRHFTLNVLNARKHIPIADAFAGRFGTFLPDKFAMGDWKTSRFGIP
ELNEATAVFHCQVNFTQIVGTHMLIIAEVDEVSEAEGARALVYCSREYRCP
>Mature_211_residues
MQHHVHTNNDKVAADTKSVLPATDWTSEPQQIETSAVTSGGGLTAAEEKAALKAKFISGMGRVAAPVSIVATDGPAGRAG
VTISAMSSVSADGDRPTLMICLNQSSRTCQALEKNRHFTLNVLNARKHIPIADAFAGRFGTFLPDKFAMGDWKTSRFGIP
ELNEATAVFHCQVNFTQIVGTHMLIIAEVDEVSEAEGARALVYCSREYRCP

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 22620; Mature: 22620

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHHVHTNNDKVAADTKSVLPATDWTSEPQQIETSAVTSGGGLTAAEEKAALKAKFISGM
CCCCEECCCCEEECCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHCC
GRVAAPVSIVATDGPAGRAGVTISAMSSVSADGDRPTLMICLNQSSRTCQALEKNRHFTL
CCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCEEE
NVLNARKHIPIADAFAGRFGTFLPDKFAMGDWKTSRFGIPELNEATAVFHCQVNFTQIVG
EEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHCC
THMLIIAEVDEVSEAEGARALVYCSREYRCP
CEEEEEEECCHHCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MQHHVHTNNDKVAADTKSVLPATDWTSEPQQIETSAVTSGGGLTAAEEKAALKAKFISGM
CCCCEECCCCEEECCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHCC
GRVAAPVSIVATDGPAGRAGVTISAMSSVSADGDRPTLMICLNQSSRTCQALEKNRHFTL
CCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCEEE
NVLNARKHIPIADAFAGRFGTFLPDKFAMGDWKTSRFGIPELNEATAVFHCQVNFTQIVG
EEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHCC
THMLIIAEVDEVSEAEGARALVYCSREYRCP
CEEEEEEECCHHCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA