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The map label for this gene is nfrA1 [H]

Identifier: 222102556

GI number: 222102556

Start: 155286

End: 156047

Strand: Reverse

Name: nfrA1 [H]

Synonym: Avi_7190

Alternate gene names: 222102556

Gene position: 156047-155286 (Counterclockwise)

Preceding gene: 222102557

Following gene: 222102549

Centisome position: 24.7

GC content: 58.27

Gene sequence:

>762_bases
TTGCCACCCAGCTGGAATGAGACGCTGGATGTGTTGCTGAACCATCGTTCCGCACGCGCCTATCTGCCCAAAGCCCTGCC
GCAAGGCACGGTGGAACTGCTGGTAGCAGCAGCACAATCGGCTCCCACCTCCTCCAATCTGCAAGCGTGGAGCGTGGTGG
CTGTTGAAGACCCGGAGCGCAAGGCGCGTCTGGAAGAATTTGCCGCTCCCAATCCGCAAATCAATGCTGCGCCGCTGTTT
CTGGTCTGGCTGGTCGATCTCGCCCGGCTAAGAGCCATTGCCCGCAGCAATGACAGCGAGGGTGCAGGACTGGATTATCT
GGAAAGCTTTGTGATTGGCGCGATTGATGCCGCCCTTGCCGCCCAGAATGCGGTGGCGGCCATTGACTCGCTTGGTCTTG
GCTCGTGTTATATCGGCGGCATCCGCAATCGGCCAGAGGATGTGGCGCGGGAATTGAATCTGCCGCCTGAAACCATCGCT
GTGTTTGGCCTCACAGTGGGCTATCCCGATCCAGCGGTAAAAACCGATGTAAAGCCGCGCCTGCCGCAATCGGCAGTGCT
GTTTCGGGAGCAATATGGCACGGTGAGTGAACAGGATCTGGCCAGCTATGATGAGCGGCTAAAAACCTTCCAGCAAGGCC
AAAATCTGCCGCAAGCGGGATGGACCAGCGTGATTGCCAAGCGCATTGGCAGTGCGAGCGCTCTCAAAGGCCGGGTTGAA
TTGACCGCCATTTTGCGGCGCTTTGGTTTTGCCTTGAAGTAA

Upstream 100 bases:

>100_bases
CATGACGGTGCATTCTGGATCACTCACTGCGGCCTCTGATGTACCGCAGGCTTCTGCCGCAGAACGCATCAAAGCCCGCT
ATGGCGTCCCGCCAAGTGTT

Downstream 100 bases:

>100_bases
AGCCCTGATAAAGCCTGTCTGGTGGCTCTCCACCAGACAGGCATCAAACTTTAGAACACCCGGCCTTCTTTCAAATGCGT
GGTGGTGCCGTTCAGGTAAT

Product: Nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPPSWNETLDVLLNHRSARAYLPKALPQGTVELLVAAAQSAPTSSNLQAWSVVAVEDPERKARLEEFAAPNPQINAAPLF
LVWLVDLARLRAIARSNDSEGAGLDYLESFVIGAIDAALAAQNAVAAIDSLGLGSCYIGGIRNRPEDVARELNLPPETIA
VFGLTVGYPDPAVKTDVKPRLPQSAVLFREQYGTVSEQDLASYDERLKTFQQGQNLPQAGWTSVIAKRIGSASALKGRVE
LTAILRRFGFALK

Sequences:

>Translated_253_residues
MPPSWNETLDVLLNHRSARAYLPKALPQGTVELLVAAAQSAPTSSNLQAWSVVAVEDPERKARLEEFAAPNPQINAAPLF
LVWLVDLARLRAIARSNDSEGAGLDYLESFVIGAIDAALAAQNAVAAIDSLGLGSCYIGGIRNRPEDVARELNLPPETIA
VFGLTVGYPDPAVKTDVKPRLPQSAVLFREQYGTVSEQDLASYDERLKTFQQGQNLPQAGWTSVIAKRIGSASALKGRVE
LTAILRRFGFALK
>Mature_252_residues
PPSWNETLDVLLNHRSARAYLPKALPQGTVELLVAAAQSAPTSSNLQAWSVVAVEDPERKARLEEFAAPNPQINAAPLFL
VWLVDLARLRAIARSNDSEGAGLDYLESFVIGAIDAALAAQNAVAAIDSLGLGSCYIGGIRNRPEDVARELNLPPETIAV
FGLTVGYPDPAVKTDVKPRLPQSAVLFREQYGTVSEQDLASYDERLKTFQQGQNLPQAGWTSVIAKRIGSASALKGRVEL
TAILRRFGFALK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=224, Percent_Identity=32.1428571428571, Blast_Score=109, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 27289; Mature: 27158

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSWNETLDVLLNHRSARAYLPKALPQGTVELLVAAAQSAPTSSNLQAWSVVAVEDPER
CCCCHHHHHHHHHCCCHHHHHCCHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHH
KARLEEFAAPNPQINAAPLFLVWLVDLARLRAIARSNDSEGAGLDYLESFVIGAIDAALA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AQNAVAAIDSLGLGSCYIGGIRNRPEDVARELNLPPETIAVFGLTVGYPDPAVKTDVKPR
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCHHHHEEEEEECCCCCCCCCCCCCCC
LPQSAVLFREQYGTVSEQDLASYDERLKTFQQGQNLPQAGWTSVIAKRIGSASALKGRVE
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHH
LTAILRRFGFALK
HHHHHHHHCCCCC
>Mature Secondary Structure 
PPSWNETLDVLLNHRSARAYLPKALPQGTVELLVAAAQSAPTSSNLQAWSVVAVEDPER
CCCHHHHHHHHHCCCHHHHHCCHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHH
KARLEEFAAPNPQINAAPLFLVWLVDLARLRAIARSNDSEGAGLDYLESFVIGAIDAALA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AQNAVAAIDSLGLGSCYIGGIRNRPEDVARELNLPPETIAVFGLTVGYPDPAVKTDVKPR
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCHHHHEEEEEECCCCCCCCCCCCCCC
LPQSAVLFREQYGTVSEQDLASYDERLKTFQQGQNLPQAGWTSVIAKRIGSASALKGRVE
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHH
LTAILRRFGFALK
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]