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The map label for this gene is potB [H]

Identifier: 222102510

GI number: 222102510

Start: 105491

End: 106360

Strand: Direct

Name: potB [H]

Synonym: Avi_7130

Alternate gene names: 222102510

Gene position: 105491-106360 (Clockwise)

Preceding gene: 222102509

Following gene: 222102511

Centisome position: 16.7

GC content: 54.6

Gene sequence:

>870_bases
ATGTCACTCTCCTCGGCAAAGCGGGTATCGCTTTTGCAAATCGCGCCTATGGCGCTGGTTCTGGTGTGTTTCGTGATCCT
GCCATTGGCGATGATTATTGCTGTCAGCTTCATGGATTACGGTTTTGCCGAGGTGATCCCGGAGTTCATCTGGGAAAATT
ATCTTGATATTTTCACCTCTGAACTGACGCTCAGGCTGTATCTCCAGACGTTCAAATTCCTGGCGATTGTCTGGGCGATC
ACGCTGTTTCTCGGCTTTACCATCGCCTATTATCTGATGTTTCATGTCCGCAGCCCAATCGTGCGGGCGCTGTGCATTGC
GGCCTGCGCCATACCGTTCTGGACATCAGGGCCAATCCGCATGGTGTCCTGGGTGCCGCTGCTGGGGCGCGAAGGGCTGG
TCAATCAGGTCTTGCTGCATCTGCATATCATAGAGCAGCCGCTCTCTTGGCTGCTCTATTCGGAATTTTCCGTCATCGTC
GTCTACGTCAATGTGCTGACGCTGGCCATGTTGGGACCGATCGCCAATTCCATGGCAAAGATTGATAAAAACCTTCTGCA
TGCCGCCCGCGATCAGGGGGCAAGTGAGGGACAGATCATCCGTCTGGTGATTATCCCACTGATCAAACCCGGCATCGCCA
TAGGCTCGATTTTTATTGTGACGGCGGTGATGGGCGATTTCTTCATCGTCAAACAGATGGGCGGCGGCCAGATCAACACG
GCGGTGGGGGCCATCGCCACCGAGCTGAACGCCTTCCAATACCCGCCCGCTGCGGCAAAATCGGTTGTGATGCTGGTCAT
TGTGCTGGGCTTCGTCGCCTCTCTGCTGCGCCTCGTTGATATCCGAAAAGAACTGGCCAATCAGGAGTGA

Upstream 100 bases:

>100_bases
GGCATCTGGAACACGCTAATGGATGAGGATCGCTATCTGGTGCGCCGCTGGCAGGATTTCATCGCCGCGTAAAATTTCAT
TCCAACCAAGGACATTTACG

Downstream 100 bases:

>100_bases
ATGATGCGCGAGCGTGGACAACCAAGACCTGCTTCCTTCTATCTGCTGTTGGGCATTTTCGCTGTCTATCTGGCCTTTCT
CTATGGGCCGATGCTGTGTG

Product: ABC transporter membrane spanning protein (spermidine/putrescine)

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSLSSAKRVSLLQIAPMALVLVCFVILPLAMIIAVSFMDYGFAEVIPEFIWENYLDIFTSELTLRLYLQTFKFLAIVWAI
TLFLGFTIAYYLMFHVRSPIVRALCIAACAIPFWTSGPIRMVSWVPLLGREGLVNQVLLHLHIIEQPLSWLLYSEFSVIV
VYVNVLTLAMLGPIANSMAKIDKNLLHAARDQGASEGQIIRLVIIPLIKPGIAIGSIFIVTAVMGDFFIVKQMGGGQINT
AVGAIATELNAFQYPPAAAKSVVMLVIVLGFVASLLRLVDIRKELANQE

Sequences:

>Translated_289_residues
MSLSSAKRVSLLQIAPMALVLVCFVILPLAMIIAVSFMDYGFAEVIPEFIWENYLDIFTSELTLRLYLQTFKFLAIVWAI
TLFLGFTIAYYLMFHVRSPIVRALCIAACAIPFWTSGPIRMVSWVPLLGREGLVNQVLLHLHIIEQPLSWLLYSEFSVIV
VYVNVLTLAMLGPIANSMAKIDKNLLHAARDQGASEGQIIRLVIIPLIKPGIAIGSIFIVTAVMGDFFIVKQMGGGQINT
AVGAIATELNAFQYPPAAAKSVVMLVIVLGFVASLLRLVDIRKELANQE
>Mature_288_residues
SLSSAKRVSLLQIAPMALVLVCFVILPLAMIIAVSFMDYGFAEVIPEFIWENYLDIFTSELTLRLYLQTFKFLAIVWAIT
LFLGFTIAYYLMFHVRSPIVRALCIAACAIPFWTSGPIRMVSWVPLLGREGLVNQVLLHLHIIEQPLSWLLYSEFSVIVV
YVNVLTLAMLGPIANSMAKIDKNLLHAARDQGASEGQIIRLVIIPLIKPGIAIGSIFIVTAVMGDFFIVKQMGGGQINTA
VGAIATELNAFQYPPAAAKSVVMLVIVLGFVASLLRLVDIRKELANQE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=34.375, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787080, Length=219, Percent_Identity=29.2237442922374, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787713, Length=256, Percent_Identity=27.34375, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31885; Mature: 31754

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLSSAKRVSLLQIAPMALVLVCFVILPLAMIIAVSFMDYGFAEVIPEFIWENYLDIFTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ELTLRLYLQTFKFLAIVWAITLFLGFTIAYYLMFHVRSPIVRALCIAACAIPFWTSGPIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MVSWVPLLGREGLVNQVLLHLHIIEQPLSWLLYSEFSVIVVYVNVLTLAMLGPIANSMAK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKNLLHAARDQGASEGQIIRLVIIPLIKPGIAIGSIFIVTAVMGDFFIVKQMGGGQINT
HHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AVGAIATELNAFQYPPAAAKSVVMLVIVLGFVASLLRLVDIRKELANQE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]