Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 222102502

GI number: 222102502

Start: 96351

End: 97313

Strand: Reverse

Name: gcvA [H]

Synonym: Avi_7121

Alternate gene names: 222102502

Gene position: 97313-96351 (Counterclockwise)

Preceding gene: 222102503

Following gene: 222102501

Centisome position: 15.4

GC content: 53.37

Gene sequence:

>963_bases
ATGAGAAGACGTGTACCTCTCAACTCCGTGCGGGCCTTTGAAGCAGCAGCAAGACGCATGAGCCTTGTGCAGGCCAGTGA
CGAATTGTGTGTGACGCCGACGGCGGTCAGTCATCAGATCCGGCAACTTGAAGATTTCCTACAAACCAAACTGTTTCTGC
GCCGCAGCAGCCGTCTGGAGCTAACGCCGGAATCGCGTGCTTGCCTTGCCAAACTTACCGAAGCACTGGACATTATAGAC
GATGCAATTGCCGCACTTCACAAACCGATGGACATGCCGGAGCGTCTGGTGGTTGGCGCATCAGCCTCCGTTGGCTCTTT
GTGGCTCATGCCAAGATTGCGGAATTTTTACCGTACAGCGCCAGAAATTGATATTTCGCTGACCACCTTCATTGAGCGTT
CAGCGATTGAAACGGACACCTCCGATGTCTGGATCTGCAACTGGCACACCAATCTGGATCGGCGCGTGGAACTCCTATTG
GAAGAGGATATTATCCCCGTTTGCTCGCCTCAGATGGCTGAGGAGTTTGGTGAGTTGAGCTTTGACTCCTTGCGAGAGTT
ACCACTTGTGCATGTGGACCGCGCCAAGGCGAGCATTAATGGTCAATATCCCGATTGGGACCGTTACTTGCGTGAATATG
GCATCAGCCGCAACGATATCGCCAAGGGGCCGCGCTTCAATCAGGCTGGCCCGGCGATTGAGGCTGCCAAAGCGGGAATG
GGGGCGCTGCTTGGGCGCTCGATTCTGATTGAGCGTGCCTTGGAAACTGGAGAACTGATCCGGTTGGGCGACCCTTATCC
AATTCGCAATCCTTATTATCTCATTTCACCCCTGCAGCCGGCCTCAAACGCCGTCAAGATGTTCAAGGACTGGATTTTTG
CTCAGGCCAAGACCAGAACTAGCACCTATGTTGAGTTGCCCCATAAAAACCGGAGAGGATCAGATTTCCGTTTGACCGTT
TAA

Upstream 100 bases:

>100_bases
TATTTTGAACAATATTGATTTTGATCAATTTTTAGACTTGCCGTTCTTCTGCGCTCATGGTCTTCTTTGATTGGGAGGAC
AATCAATAGGGGACGTTGGC

Downstream 100 bases:

>100_bases
TCCCAACGATCCTGGTGTTTGATCCCGGCATCTAGTTGTGCATTATTTTGGCGTTGGTGCACGGACCAAATTCCAACAGT
TCTCGCCTATGTGGCTGCGA

Product: transcriptional regulator LysR family

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRRRVPLNSVRAFEAAARRMSLVQASDELCVTPTAVSHQIRQLEDFLQTKLFLRRSSRLELTPESRACLAKLTEALDIID
DAIAALHKPMDMPERLVVGASASVGSLWLMPRLRNFYRTAPEIDISLTTFIERSAIETDTSDVWICNWHTNLDRRVELLL
EEDIIPVCSPQMAEEFGELSFDSLRELPLVHVDRAKASINGQYPDWDRYLREYGISRNDIAKGPRFNQAGPAIEAAKAGM
GALLGRSILIERALETGELIRLGDPYPIRNPYYLISPLQPASNAVKMFKDWIFAQAKTRTSTYVELPHKNRRGSDFRLTV

Sequences:

>Translated_320_residues
MRRRVPLNSVRAFEAAARRMSLVQASDELCVTPTAVSHQIRQLEDFLQTKLFLRRSSRLELTPESRACLAKLTEALDIID
DAIAALHKPMDMPERLVVGASASVGSLWLMPRLRNFYRTAPEIDISLTTFIERSAIETDTSDVWICNWHTNLDRRVELLL
EEDIIPVCSPQMAEEFGELSFDSLRELPLVHVDRAKASINGQYPDWDRYLREYGISRNDIAKGPRFNQAGPAIEAAKAGM
GALLGRSILIERALETGELIRLGDPYPIRNPYYLISPLQPASNAVKMFKDWIFAQAKTRTSTYVELPHKNRRGSDFRLTV
>Mature_320_residues
MRRRVPLNSVRAFEAAARRMSLVQASDELCVTPTAVSHQIRQLEDFLQTKLFLRRSSRLELTPESRACLAKLTEALDIID
DAIAALHKPMDMPERLVVGASASVGSLWLMPRLRNFYRTAPEIDISLTTFIERSAIETDTSDVWICNWHTNLDRRVELLL
EEDIIPVCSPQMAEEFGELSFDSLRELPLVHVDRAKASINGQYPDWDRYLREYGISRNDIAKGPRFNQAGPAIEAAKAGM
GALLGRSILIERALETGELIRLGDPYPIRNPYYLISPLQPASNAVKMFKDWIFAQAKTRTSTYVELPHKNRRGSDFRLTV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=308, Percent_Identity=31.4935064935065, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786448, Length=303, Percent_Identity=30.03300330033, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=28.7625418060201, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36312; Mature: 36312

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRVPLNSVRAFEAAARRMSLVQASDELCVTPTAVSHQIRQLEDFLQTKLFLRRSSRLE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LTPESRACLAKLTEALDIIDDAIAALHKPMDMPERLVVGASASVGSLWLMPRLRNFYRTA
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
PEIDISLTTFIERSAIETDTSDVWICNWHTNLDRRVELLLEEDIIPVCSPQMAEEFGELS
CCCEEHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
FDSLRELPLVHVDRAKASINGQYPDWDRYLREYGISRNDIAKGPRFNQAGPAIEAAKAGM
HHHHHCCCEEEECCHHHHCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHH
GALLGRSILIERALETGELIRLGDPYPIRNPYYLISPLQPASNAVKMFKDWIFAQAKTRT
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
STYVELPHKNRRGSDFRLTV
CEEEECCCCCCCCCCEEEEC
>Mature Secondary Structure
MRRRVPLNSVRAFEAAARRMSLVQASDELCVTPTAVSHQIRQLEDFLQTKLFLRRSSRLE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LTPESRACLAKLTEALDIIDDAIAALHKPMDMPERLVVGASASVGSLWLMPRLRNFYRTA
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
PEIDISLTTFIERSAIETDTSDVWICNWHTNLDRRVELLLEEDIIPVCSPQMAEEFGELS
CCCEEHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
FDSLRELPLVHVDRAKASINGQYPDWDRYLREYGISRNDIAKGPRFNQAGPAIEAAKAGM
HHHHHCCCEEEECCHHHHCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHH
GALLGRSILIERALETGELIRLGDPYPIRNPYYLISPLQPASNAVKMFKDWIFAQAKTRT
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
STYVELPHKNRRGSDFRLTV
CEEEECCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]