BacMap

Definition	Agrobacterium vitis S4 plasmid pAtS4e, complete sequence.
Accession	NC_011981
Length	631,775

Click here to switch to the map view.

The map label for this gene is rbsA [H]

Identifier: 222102481

GI number: 222102481

Start: 79081

End: 79842

Strand: Direct

Name: rbsA [H]

Synonym: Avi_7088

Alternate gene names: 222102481

Gene position: 79081-79842 (Clockwise)

Preceding gene: 222102480

Following gene: 222102482

Centisome position: 12.52

GC content: 56.69

Gene sequence:

>762_bases
ATGCATAGGTCGTCTGAGGCTCAGGACAATGTCATTCTGCGCATCGACAATCTGAACAAGAGCTTTCAAGCCCTCCATGT
CACTCGTCAGGTGACATTTGATTTGTTGAGCGGAGAATGTGTCGGCCTCATTGGGCCGAATGGTGCGGGAAAAAGCACTC
TGCTGGGCCTGCTTTCGGGCAGTCTCAGGCCGGATTCCGGAACACTCACGCTGGATGGTGCCAACGTAACCCCGTTTGCG
CAATTCAAAAGGGTTCGCAGCGGGATTGCCAAGGCAAGCCAGATTCCCCAGACGTTTGATCGCCTGCTGGTGTTCGACAA
TGTTCGGGTTGCGGCGCTGTATGGGGCTGGAATCCGTGAAAAGGAAGCGGCAGACTGGACAGCGGATGTTCTGAGCCAGT
GTGGGCTTGGCGATAAAGCGCGGCGCGAAACCCGCACCCTTGGCCTTCTGGATCGAAAACGGCTGGAACTGGCAAAGGCC
GTGGCCGCACGTCCGCGCGTGTTGTTACTCGATGAAGTGGCAGCCGGACTGACGGAGCCAGAAATTCTCGAAATCGTGAA
GCTGGTCGAGCGGTTAAAGCCGGGTCGTGCGACGGTTTGGGTTGAGCATATTCCCTATGCCCTTCGAGATGTCTGCGAGC
GCCTCATCCTGATGGATGCCGGGGCCAAGCTGCTCGATGGTCCATTCCACACCGTCTGGCAGGATCCGAAATTGCATCAG
ATCTACATGGGGGTTCCCGATGGCACCACTTTTGACGTTTGA

Upstream 100 bases:

>100_bases
CGCTTTGATGCGGCAGCGAAATTTCCGTTTGATCTGGATATTGTTTACAATGCTCTGGCCCCGGAAATTCCAGTCACAGC
ACACCTCCGGCGCCTTCAAA

Downstream 100 bases:

>100_bases
CGATCTGGTTGTGCGCTATGGCGACATTCCTGCGCTGGCGGGGGTCAGTTTTGCCATGGATGAGGGAGAAACCCTTGCAC
TTGTCGGGGCCAATGGTGCC

Product: ABC transporter nucleotide binding/ATPase protein (ribose)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MHRSSEAQDNVILRIDNLNKSFQALHVTRQVTFDLLSGECVGLIGPNGAGKSTLLGLLSGSLRPDSGTLTLDGANVTPFA
QFKRVRSGIAKASQIPQTFDRLLVFDNVRVAALYGAGIREKEAADWTADVLSQCGLGDKARRETRTLGLLDRKRLELAKA
VAARPRVLLLDEVAAGLTEPEILEIVKLVERLKPGRATVWVEHIPYALRDVCERLILMDAGAKLLDGPFHTVWQDPKLHQ
IYMGVPDGTTFDV

Sequences:

>Translated_253_residues
MHRSSEAQDNVILRIDNLNKSFQALHVTRQVTFDLLSGECVGLIGPNGAGKSTLLGLLSGSLRPDSGTLTLDGANVTPFA
QFKRVRSGIAKASQIPQTFDRLLVFDNVRVAALYGAGIREKEAADWTADVLSQCGLGDKARRETRTLGLLDRKRLELAKA
VAARPRVLLLDEVAAGLTEPEILEIVKLVERLKPGRATVWVEHIPYALRDVCERLILMDAGAKLLDGPFHTVWQDPKLHQ
IYMGVPDGTTFDV
>Mature_253_residues
MHRSSEAQDNVILRIDNLNKSFQALHVTRQVTFDLLSGECVGLIGPNGAGKSTLLGLLSGSLRPDSGTLTLDGANVTPFA
QFKRVRSGIAKASQIPQTFDRLLVFDNVRVAALYGAGIREKEAADWTADVLSQCGLGDKARRETRTLGLLDRKRLELAKA
VAARPRVLLLDEVAAGLTEPEILEIVKLVERLKPGRATVWVEHIPYALRDVCERLILMDAGAKLLDGPFHTVWQDPKLHQ
IYMGVPDGTTFDV

Specific function: Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27262624, Length=205, Percent_Identity=28.780487804878, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27262626, Length=205, Percent_Identity=28.780487804878, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI153792144, Length=178, Percent_Identity=29.7752808988764, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI45446740, Length=220, Percent_Identity=25.9090909090909, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI47078218, Length=220, Percent_Identity=25.9090909090909, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI116734710, Length=225, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI27436953, Length=178, Percent_Identity=25.8426966292135, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI27881501, Length=211, Percent_Identity=25.1184834123223, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI30795238, Length=211, Percent_Identity=25.1184834123223, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI27477115, Length=169, Percent_Identity=29.585798816568, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1789864, Length=249, Percent_Identity=28.5140562248996, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789593, Length=229, Percent_Identity=28.3842794759825, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790525, Length=225, Percent_Identity=28, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787029, Length=228, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788225, Length=234, Percent_Identity=28.6324786324786, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87082268, Length=227, Percent_Identity=27.3127753303965, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081791, Length=234, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789672, Length=215, Percent_Identity=25.1162790697674, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787792, Length=223, Percent_Identity=27.8026905829596, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787370, Length=232, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1788210, Length=231, Percent_Identity=25.5411255411255, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48995001, Length=219, Percent_Identity=27.3972602739726, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786398, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790544, Length=239, Percent_Identity=30.1255230125523, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788761, Length=230, Percent_Identity=28.695652173913, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788472, Length=227, Percent_Identity=23.7885462555066, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790190, Length=231, Percent_Identity=22.0779220779221, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790162, Length=208, Percent_Identity=28.3653846153846, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790467, Length=217, Percent_Identity=27.6497695852535, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87081782, Length=232, Percent_Identity=28.448275862069, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789991, Length=201, Percent_Identity=28.8557213930348, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786872, Length=228, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786698, Length=183, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787089, Length=216, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790739, Length=222, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789873, Length=200, Percent_Identity=31, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789032, Length=211, Percent_Identity=29.8578199052133, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI48994943, Length=214, Percent_Identity=30.3738317757009, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87081834, Length=206, Percent_Identity=28.6407766990291, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787712, Length=213, Percent_Identity=25.3521126760563, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786319, Length=171, Percent_Identity=28.6549707602339, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787105, Length=227, Percent_Identity=25.9911894273128, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=27.6497695852535, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786253, Length=165, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789891, Length=213, Percent_Identity=28.6384976525822, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786563, Length=164, Percent_Identity=29.8780487804878, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082267, Length=229, Percent_Identity=26.2008733624454, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786345, Length=228, Percent_Identity=27.6315789473684, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367384, Length=60, Percent_Identity=48.3333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789751, Length=172, Percent_Identity=31.9767441860465, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693107, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=221, Percent_Identity=30.316742081448, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193211017, Length=202, Percent_Identity=28.7128712871287, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71996809, Length=202, Percent_Identity=28.7128712871287, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193211015, Length=202, Percent_Identity=28.7128712871287, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI193202349, Length=201, Percent_Identity=25.8706467661692, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25146777, Length=202, Percent_Identity=25.7425742574257, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI212646699, Length=202, Percent_Identity=25.2475247524752, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71984940, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565586, Length=218, Percent_Identity=28.8990825688073, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17558664, Length=228, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=179, Percent_Identity=31.2849162011173, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=179, Percent_Identity=31.2849162011173, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=179, Percent_Identity=31.2849162011173, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193209708, Length=228, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17569143, Length=204, Percent_Identity=27.9411764705882, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322640, Length=236, Percent_Identity=24.5762711864407, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24666092, Length=191, Percent_Identity=27.2251308900524, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648314, Length=228, Percent_Identity=24.5614035087719, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24643674, Length=192, Percent_Identity=26.0416666666667, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221512771, Length=228, Percent_Identity=22.8070175438596, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI85724864, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580555, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI85816269, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580553, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007184, Length=209, Percent_Identity=26.7942583732057, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221500365, Length=209, Percent_Identity=26.7942583732057, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920532, Length=204, Percent_Identity=30.8823529411765, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580930, Length=204, Percent_Identity=30.8823529411765, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21357907, Length=183, Percent_Identity=28.9617486338798, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28574533, Length=245, Percent_Identity=26.1224489795918, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27729; Mature: 27729

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRSSEAQDNVILRIDNLNKSFQALHVTRQVTFDLLSGECVGLIGPNGAGKSTLLGLLSG
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHC
SLRPDSGTLTLDGANVTPFAQFKRVRSGIAKASQIPQTFDRLLVFDNVRVAALYGAGIRE
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCC
KEAADWTADVLSQCGLGDKARRETRTLGLLDRKRLELAKAVAARPRVLLLDEVAAGLTEP
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCH
EILEIVKLVERLKPGRATVWVEHIPYALRDVCERLILMDAGAKLLDGPFHTVWQDPKLHQ
HHHHHHHHHHHCCCCCEEEEHHHCCHHHHHHHHHHHHHHCCCHHHCCCCHHHCCCCCEEE
IYMGVPDGTTFDV
EEEECCCCCEECC
>Mature Secondary Structure
MHRSSEAQDNVILRIDNLNKSFQALHVTRQVTFDLLSGECVGLIGPNGAGKSTLLGLLSG
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHC
SLRPDSGTLTLDGANVTPFAQFKRVRSGIAKASQIPQTFDRLLVFDNVRVAALYGAGIRE
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCC
KEAADWTADVLSQCGLGDKARRETRTLGLLDRKRLELAKAVAARPRVLLLDEVAAGLTEP
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCH
EILEIVKLVERLKPGRATVWVEHIPYALRDVCERLILMDAGAKLLDGPFHTVWQDPKLHQ
HHHHHHHHHHHCCCCCEEEEHHHCCHHHHHHHHHHHHHHCCCHHHCCCCHHHCCCCCEEE
IYMGVPDGTTFDV
EEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]