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The map label for this gene is mdh [C]

Identifier: 222102420

GI number: 222102420

Start: 11936

End: 12985

Strand: Reverse

Name: mdh [C]

Synonym: Avi_7014

Alternate gene names: 222102420

Gene position: 12985-11936 (Counterclockwise)

Preceding gene: 222102429

Following gene: 222102419

Centisome position: 2.06

GC content: 61.14

Gene sequence:

>1050_bases
ATGACATTGAAAACGAATTTCGCAGCGGGAACCTGCCGCCTGCCGTTCGAAGCCCTTCAGAACCTCCTCAAGCGCATTTT
CCTGGCGGCTGGAGCCAGCGAGCACGTGGCGAACATCCTCAGCGCCAATTGTGCTGGATGCGAACGCGATGGCGCGCTTT
CACATGGCATTTTCAGAATGCCCGGCTATGTAAGTTCCATCCGCAGCGGTTGGGTCGATCCTGCCGCCGTGCCGGTTCTG
GAGACCGGTGGCTCGGGCTTCCTGCGGGTTGATGCCCGTAACGGATTTACGCAGGTCGCGCTCGATTTTGCGGCACAGTC
TTTGAAGGAGCGCGCAAGGCAGGAAGGCATCGCGCTGCTTTCGATCCGCAATTCCCATCATTTCAGCGCGCTCTGGCCGG
ATGTCGTGCCCTTCGCCGAAGAGCGGCTACTGGCCATCGCGATGGTCAACAGCTTTGCCTGTACCGCGCCTTTCGATGCC
AAGGTGCCGGTTTTCGGCACCAATCCGGTTGCCTTTGCCGCGCCCTGTGACGGCATGGTCCCCATGGTCGTCGATATGGC
CACGAGCGCCATCGCCAATGGCGATGTGCAGATTGCCGCGCGCGAAGGCTTCAGCCTGCCGCCTGGGCTAGGCATCGACC
GGAGCGGAAAGCCAACCGTCGATCCGCATGCGGTGCTGGATGGCGGCGCACTGCTGACATTCGGCGGCTATAAGGGCTCG
GCCATTTCGATGCTGGTGGAAGTGATGAGCGCCGCTTTGACCGGAGGCAAGTTTTCCTACGAAGTGGACTGGTCCGACTA
TCCCGGGGCACAGACGCCGCATACGGGCCAGCTCGTTATTCTGATCGATCCGGATGTTGGCCGCAATGCGCAATTCAGCC
GCCGCGTCCGGCATCTGGTCGAACGGCTCCGACTGGCAAAGGTCTCTCGCCTGCCAGGCGATCGCCGGCTGGTCACACGC
GAGCGTTCGATGCGGGAGGGCGTGTGCCTACAAAAGAGCGATCTGGAGAGAATGGAACTTCTAGCGCAGCAAAGATGGGC
ATCAACCTGA

Upstream 100 bases:

>100_bases
TCGAACAAACTTTACTCATAGGCGGTCAAGGAATAATCCGCTTGTGATTACATCATTTCGCGCCTATCTTTCCTATAAGA
AACATTTGCGAAGTTGCCCT

Downstream 100 bases:

>100_bases
TGTCGCGGAGCGGGGATCAGGTTGATCCTTGCAGAAGTTGCGGCTCTGTGGCATGGGCCATCGGAAGTTACATAAGAAAC
AATGTCTACAAGTGATGCAC

Product: malate dehydrogenase

Products: oxaloacetate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTLKTNFAAGTCRLPFEALQNLLKRIFLAAGASEHVANILSANCAGCERDGALSHGIFRMPGYVSSIRSGWVDPAAVPVL
ETGGSGFLRVDARNGFTQVALDFAAQSLKERARQEGIALLSIRNSHHFSALWPDVVPFAEERLLAIAMVNSFACTAPFDA
KVPVFGTNPVAFAAPCDGMVPMVVDMATSAIANGDVQIAAREGFSLPPGLGIDRSGKPTVDPHAVLDGGALLTFGGYKGS
AISMLVEVMSAALTGGKFSYEVDWSDYPGAQTPHTGQLVILIDPDVGRNAQFSRRVRHLVERLRLAKVSRLPGDRRLVTR
ERSMREGVCLQKSDLERMELLAQQRWAST

Sequences:

>Translated_349_residues
MTLKTNFAAGTCRLPFEALQNLLKRIFLAAGASEHVANILSANCAGCERDGALSHGIFRMPGYVSSIRSGWVDPAAVPVL
ETGGSGFLRVDARNGFTQVALDFAAQSLKERARQEGIALLSIRNSHHFSALWPDVVPFAEERLLAIAMVNSFACTAPFDA
KVPVFGTNPVAFAAPCDGMVPMVVDMATSAIANGDVQIAAREGFSLPPGLGIDRSGKPTVDPHAVLDGGALLTFGGYKGS
AISMLVEVMSAALTGGKFSYEVDWSDYPGAQTPHTGQLVILIDPDVGRNAQFSRRVRHLVERLRLAKVSRLPGDRRLVTR
ERSMREGVCLQKSDLERMELLAQQRWAST
>Mature_348_residues
TLKTNFAAGTCRLPFEALQNLLKRIFLAAGASEHVANILSANCAGCERDGALSHGIFRMPGYVSSIRSGWVDPAAVPVLE
TGGSGFLRVDARNGFTQVALDFAAQSLKERARQEGIALLSIRNSHHFSALWPDVVPFAEERLLAIAMVNSFACTAPFDAK
VPVFGTNPVAFAAPCDGMVPMVVDMATSAIANGDVQIAAREGFSLPPGLGIDRSGKPTVDPHAVLDGGALLTFGGYKGSA
ISMLVEVMSAALTGGKFSYEVDWSDYPGAQTPHTGQLVILIDPDVGRNAQFSRRVRHLVERLRLAKVSRLPGDRRLVTRE
RSMREGVCLQKSDLERMELLAQQRWAST

Specific function: Unknown

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787020, Length=329, Percent_Identity=29.1793313069909, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786727, Length=305, Percent_Identity=30.8196721311475, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1790000, Length=342, Percent_Identity=26.0233918128655, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17507179, Length=346, Percent_Identity=28.9017341040462, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17536633, Length=295, Percent_Identity=26.7796610169492, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24667904, Length=263, Percent_Identity=27.7566539923954, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24667908, Length=291, Percent_Identity=26.8041237113402, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45553211, Length=291, Percent_Identity=26.8041237113402, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24667912, Length=291, Percent_Identity=26.8041237113402, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24648360, Length=311, Percent_Identity=24.7588424437299, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24658010, Length=338, Percent_Identity=21.301775147929, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: 1.1.1.37

Molecular weight: Translated: 37538; Mature: 37406

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKTNFAAGTCRLPFEALQNLLKRIFLAAGASEHVANILSANCAGCERDGALSHGIFRM
CCEECCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PGYVSSIRSGWVDPAAVPVLETGGSGFLRVDARNGFTQVALDFAAQSLKERARQEGIALL
CCHHHHHHHCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEE
SIRNSHHFSALWPDVVPFAEERLLAIAMVNSFACTAPFDAKVPVFGTNPVAFAAPCDGMV
EEECCCCCHHHCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEECCCCEEEECCCCCCH
PMVVDMATSAIANGDVQIAAREGFSLPPGLGIDRSGKPTVDPHAVLDGGALLTFGGYKGS
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCH
AISMLVEVMSAALTGGKFSYEVDWSDYPGAQTPHTGQLVILIDPDVGRNAQFSRRVRHLV
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ERLRLAKVSRLPGDRRLVTRERSMREGVCLQKSDLERMELLAQQRWAST
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLKTNFAAGTCRLPFEALQNLLKRIFLAAGASEHVANILSANCAGCERDGALSHGIFRM
CEECCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PGYVSSIRSGWVDPAAVPVLETGGSGFLRVDARNGFTQVALDFAAQSLKERARQEGIALL
CCHHHHHHHCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEE
SIRNSHHFSALWPDVVPFAEERLLAIAMVNSFACTAPFDAKVPVFGTNPVAFAAPCDGMV
EEECCCCCHHHCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEECCCCEEEECCCCCCH
PMVVDMATSAIANGDVQIAAREGFSLPPGLGIDRSGKPTVDPHAVLDGGALLTFGGYKGS
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCH
AISMLVEVMSAALTGGKFSYEVDWSDYPGAQTPHTGQLVILIDPDVGRNAQFSRRVRHLV
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ERLRLAKVSRLPGDRRLVTRERSMREGVCLQKSDLERMELLAQQRWAST
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-malate; NAD+

Specific reaction: (S)-malate + NAD+ = oxaloacetate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]