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The map label for this gene is rnjB [H]

Identifier: 222097243

GI number: 222097243

Start: 3541182

End: 3542714

Strand: Reverse

Name: rnjB [H]

Synonym: BCQ_3583

Alternate gene names: 222097243

Gene position: 3542714-3541182 (Counterclockwise)

Preceding gene: 222097244

Following gene: 222097242

Centisome position: 67.94

GC content: 36.4

Gene sequence:

>1533_bases
ATGTTTGGAATTGATATCGTTATTCCTGATATTACATATTTAGTAGAAAACCAAGAGCGAGTAAAAGGTTTATTTATTAC
TCACGGTCATGAAGATCATATTGGTGGCATTGTTTACGTACTTCGTAAATTATCAATTCCAGTGTATGCAACGAAATTAA
CGGTAGGACTTATACAAGAAAAACTTGGCGAAGCGGGTATGTTAGGCCGTGTAGATTTAAAAACAATCGATTCAAATTCA
ACAGTAGAGTTCAATACTACAACGGTGTCATTCTTTGGAACGACACATAGTATTCCAGATTCTGTTGGTGTTTGTTTCCA
TACATCAAAAGGTGCGATCGTATATACGGGAGACTTTAAATTCGATCAAACTCCAATTGGAAATAGTGGAGCAGACCTTG
GGAAAATGGCTCAAATCGGAAATGAAGGCGTTCTATGCTTATTATCTGATAGTACAAATGCTGAGCGTCCAGGCTATACA
GGTTCTGAAAAAGAAGTTGGTGTAGAAATTTCTAAAGTGTTCTACGGCGCAGAAGGACGTATTATCGTTGCTTCATTTGC
ATCGAATGTACATCGTATTCAACAAGTATTTGATGCAGCTGCTGAAACAGGACGAAAAGTAGCGGTTGTAGGACGTAGTA
TGGTGAAAGTGGTAGATATCGCAAGACGTCTTGGTTATTTAGATGTTCCAGAAGGTATGGTTATTTCACTACAAGAAGTA
GACAATTTCCCAGAGAAAAAGGTAGCTATTTTAACGACAGGTAGCCAAGGTGAACCGATGGCAGCCCTTTCTAGAATGGC
AAAGCAAGCTCATAAACAAATTTCAATTCGTAAAGGTGATACGGTTATTATCGCGGCATCTCCAATTCCAGGTAATGAAA
TATCTGTATCAAAAATTATTGACTTGTTATTTAGAGCGGGAGCTGAAGTTGTTTATTACGGTGAAAGAAAAGTTCACGTT
TCAGGTCACGGTAGCCAAGAAGAATTAAAATTAATGTTAAATTTAATGAAACCGAAATATTTTGTACCCGTACATGGTGA
GTTCCGTATGCAAAAAGCACATGCATACTTAGCTGAAGATGTAGGTATTGCAAGAGAAAATATCTTTATTGTAGAAAAAG
GCGATGTAATTGGTTTTGGTGATGATGAAGCGAACTTAGTTGGCAAAGTGCAAGCTGGCAATGTGTTAATTGATGGATTA
GGTGTAGGTGATGTTGGTAATATCGTTCTTCGAGATAGAAAAATGTTATCTCAAGATGGAATATTAGTAGTTGTTGTAAC
ACTGGGTAAAGACGAAAAGAAAATTATCTCTGGTCCAGAAATCATTTCACGTGGATTTGTATATGTACGTGAATCAGAAG
CGTTAATTGACCGTTCTACAGAGATTGTACGTATGATTGTTGAGCAATCAATTAAAGAATATTCAATTGAATGGTCTATG
TTAAAACAAAATATTCGTGAATTATTAGGACAGTTCTTGTACGAAGAGACGAAGAGAAAACCTATGATTTTACCGATTAT
TATGGAAGTATAA

Upstream 100 bases:

>100_bases
TCTTGGTGGAGTAGGTGAAATCGGGAAAAACATGTACTGTGTTGAAATTGATTCTGAAATCTTTATTGTAGATGCAGGGT
TAATGTTCCCGGGAGATGAA

Downstream 100 bases:

>100_bases
AAAAGATCACCTTTACGGGTGATCTTTTTTCTTTTTTCACTCTATCGAATGAAATTTATAACTTAAGAAATACTAATTAT
ATACGAGTGAAAGGGGATGC

Product: metallo-beta-lactamase

Products: NA

Alternate protein names: RNase J2 [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MFGIDIVIPDITYLVENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNS
TVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYT
GSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEV
DNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHV
SGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAEDVGIARENIFIVEKGDVIGFGDDEANLVGKVQAGNVLIDGL
GVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKKIISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSM
LKQNIRELLGQFLYEETKRKPMILPIIMEV

Sequences:

>Translated_510_residues
MFGIDIVIPDITYLVENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNS
TVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYT
GSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEV
DNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHV
SGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAEDVGIARENIFIVEKGDVIGFGDDEANLVGKVQAGNVLIDGL
GVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKKIISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSM
LKQNIRELLGQFLYEETKRKPMILPIIMEV
>Mature_510_residues
MFGIDIVIPDITYLVENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNS
TVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYT
GSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEV
DNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHV
SGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAEDVGIARENIFIVEKGDVIGFGDDEANLVGKVQAGNVLIDGL
GVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKKIISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSM
LKQNIRELLGQFLYEETKRKPMILPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA [H]

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 55871; Mature: 55871

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGIDIVIPDITYLVENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQE
CCEEEEEECCEEEEECCCHHHCEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHH
KLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFK
HHCCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCE
FDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGR
ECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
IIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEV
EEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHH
DNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKII
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEHHHHH
DLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAED
HHHHHCCCCEEEECCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
VGIARENIFIVEKGDVIGFGDDEANLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDG
CCCCCCCEEEEECCCEEEECCCCCCEEEEEEECCEEEEECCCCCCCCEEEECHHHHCCCC
ILVVVVTLGKDEKKIISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSM
EEEEEEEECCCCHHHCCCHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LKQNIRELLGQFLYEETKRKPMILPIIMEV
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MFGIDIVIPDITYLVENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQE
CCEEEEEECCEEEEECCCHHHCEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHH
KLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFK
HHCCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCE
FDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGR
ECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
IIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEV
EEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHH
DNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKII
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEHHHHH
DLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAED
HHHHHCCCCEEEECCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
VGIARENIFIVEKGDVIGFGDDEANLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDG
CCCCCCCEEEEECCCEEEECCCCCCEEEEEEECCEEEEECCCCCCCCEEEECHHHHCCCC
ILVVVVTLGKDEKKIISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSM
EEEEEEEECCCCHHHCCCHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LKQNIRELLGQFLYEETKRKPMILPIIMEV
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]