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The map label for this gene is miaB [H]

Identifier: 222097218

GI number: 222097218

Start: 3513088

End: 3514617

Strand: Reverse

Name: miaB [H]

Synonym: BCQ_3558

Alternate gene names: 222097218

Gene position: 3514617-3513088 (Counterclockwise)

Preceding gene: 222097219

Following gene: 222097217

Centisome position: 67.4

GC content: 36.47

Gene sequence:

>1530_bases
ATGAACGAGCAACAACGATTAGCAAGTCAACAAGTGAATTCCTCTACGAAAAAAGAAGAGAAAGATTATAGCAAATACTT
TGAAAGTGTATACCAACCACCTTCCTTAAAAGACGCGAAAAAGCGAGGGAAAGAAGAAGTTAAAATTGAACGGGATTTTG
GCTTACCTGAAGAGTTTCGCAATTTCGGTACAGGAAGAAAGTTTTATATTCGTACATATGGTTGTCAAATGAATGAGCAC
GATACAGAAGTAATGGCGGGTATTTTCACTGCACTTGGATATGAACCAACCTTTTCAACTGAAGAAGCAGATGTAGTATT
ATTAAATACTTGTGCTATTCGTGAAAATGCTGAAAATAAAGTGTTCGGTGAACTAGGGCATTTAAAGGCACTAAAACGAA
GAAATCCTGATCTTTTAATTGGTGTATGTGGTTGTATGTCCCAAGAGGAATCTGTTGTAAATAAAATTATGCAAAAAAAT
CAGCATGTAGATATGGTGTTTGGAACGCATAATATTCATAGATTACCGTACATTCTAAAAGATGCAATGTTCTCGAAGGA
GACGGTAGTTGAAGTTTGGTCCAAAGAAGGAGACGTAATTGAAAATCTTCCGAAAGTACGTCGTGGTGATATTAAAGCTT
GGGTAAATATTATGTATGGCTGTGATAAGTTCTGTACATATTGTATCGTACCGTATACACGCGGAAAAGAGCGAAGCAGA
CGTCCAGAAGACATTATCCAAGAGATTCGTCATTTAGCTGCGAATGGTTATAAAGAAATTACGTTACTTGGACAGAACGT
AAATGCATATGGTAAAGACTTTGAAGATATAGAATACGGTCTTGGCGATTTAATGGACGAACTCCGTAAAGTTGATATTG
CCCGTATTCGTTTTACAACGAGCCATCCACGCGATTTCGATGATCACTTAATTGAAGTGCTTGGAAAAGGTGGCAACTTA
GTAGAACATATTCACTTACCAGTTCAATCTGGAAGCACAGAAATGTTAAAAATTATGGCGCGTAAATATTCACGTGAGCA
TTATTTAGAGCTTGTAAGAAAAATTAAAGAAGCAATTCCAAATGCGGTATTAACAACTGATATTATCGTCGGTTTCCCGA
ATGAAACAGATGAGCAATTTGAAGAAACGATGTCGTTATATCGTGAAGTAGGGTTTGATACTGCCTTTACCTTTATTTAT
TCTCCGCGTGAAGGTACACCAGCTGCAAAAATGAAAGACAATGTACCGATGGAAGTAAAGAAAGAACGTCTGCAACGCTT
AAATGCATTAGTAAATAAATTGGCAATTGAAAAGAATAATCGTTATAAAGGTCAAATTGTAGAAGTGTTAGTTGATGGGG
AAAGTAAAAATAATCCAGAAGTACTTGCAGGTTATACTCGTACGAATAAACTTGTAAATTTCGTTGCTCCAAAGTCTTTA
ATTGGTCAGCTTGTGAAAGTAAAAGTAACGGATGCAAAAACATGGTCTCTTAACGGAGAATTAGTTGAAGAGCCGATTGA
GGTGGAATAA

Upstream 100 bases:

>100_bases
ACAAGTATCTCTCAAGCTATCTAACCATTTTTGAGGTGGGTCTAAGCGCATTAAAATGGACTACAAAGTGTGTATAGTTA
AGGGTGAAAGGAGATTACCG

Downstream 100 bases:

>100_bases
TAGATGAAAGTATATTCGAAAGATGAAATTGTTGAGCAAGCGAAAGAATTAGCAAAAATGATTTCTGAAACAGAAGAAGT
TGATTTCTTTAAGAAAGCAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEEADVVLLNTCAIRENAENKVFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNNRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE

Sequences:

>Translated_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEEADVVLLNTCAIRENAENKVFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNNRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
>Mature_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEEADVVLLNTCAIRENAENKVFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNNRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=37.6811594202899, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=37.6811594202899, Blast_Score=308, Evalue=6e-84,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=28.5714285714286, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=45.2272727272727, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=27.5330396475771, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=35.2083333333333, Blast_Score=271, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=307, Percent_Identity=31.2703583061889, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21356207, Length=550, Percent_Identity=34.9090909090909, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=31.2910284463895, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58341; Mature: 58341

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEEADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNNRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
HHHHHEEEEECCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEEADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNNRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
HHHHHEEEEECCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA