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The map label for this gene is pepT

Identifier: 222097197

GI number: 222097197

Start: 3486449

End: 3487687

Strand: Reverse

Name: pepT

Synonym: BCQ_3537

Alternate gene names: 222097197

Gene position: 3487687-3486449 (Counterclockwise)

Preceding gene: 222097199

Following gene: 222097193

Centisome position: 66.89

GC content: 35.92

Gene sequence:

>1239_bases
ATGAATTTGAAACAAGAACTTATTGAAAGATTTACGAGATATGTAAAGATTGATACGCAATCAAATGAAGACAGTCATAC
AGTGCCAACAACACCAGGACAAATTGAGTTTGGTAAGTTATTAGTTGAAGAGTTAAAAGAAATTGGGTTAACAGAAGTAA
CGATGGACGATAACGGATATGTAATGGCAACACTTCCGGCTAATACGGATAAGGATGTTCCTGTAATCGGATTTTTAGCA
CATTTAGATACAGCAACAGACTTTACTGGGAAAAATGTAAAACCACAAATTCATGAAAACTTTGACGGGAACGCAATTAC
ATTAAATGAAGAGCTTAATGTTGTATTAACACCAGAACAGTTCCCAGAATTACCGTCTTATAAAGGTCATACGATTATTA
CAACGGACGGTACAACACTGTTAGGAGCAGATGATAAAGCTGGTCTTACAGAAATTATGGTTGCAATGAATTATTTAATA
CATAATCCGCAAATTAAACATGGGAAAATTAGAGTTGCGTTTACGCCGGATGAAGAAATTGGCCGCGGCCCAGCGCATTT
TGATGTAGAAGCGTTTGGCGCTTCATTTGCTTATACGATGGATGGAGGTCCATTAGGTGGTTTAGAGTATGAAAGCTTTA
ATGCTGCAGGTGCTAAGTTAACGTTTAACGGAACAAATACACATCCTGGAACAGCGAAAAATAAAATGCGTAACGCAACT
AAACTTGCTATGGAGTTTAATAGCTATTTACCAGTAGAAGAGGCACCAGAATATACAGAGGGGTATGAAGGATTCTATCA
TTTACTTTCTTTAAATGGTGATGTTGAGCAAAGTAAAGCGTATTATATTATTCGAGATTTTGATCGTGAAAATTTTGAAG
CGCGTAAACATAACGTTGAAAATATTGTGAAGCAAATGCAAGAGAAGTATGGACGAGATGCAGTCGTTTTAGAAATGAAT
GATCAATACTATAACATGCTTGAAAAGATTGAACCAGTGAGAGAAATTGTTGATATTGCATATGAGGCAATGAAAAGTTT
AAATATCGAACCAAATATTCATCCGATTCGCGGTGGAACAGATGGATCACAATTATCGTATATGGGATTACCAACCCCAA
ACATTTTCACAGGTGGTGAAAACTATCACGGTAAATTTGAGTATGTTTCGGTAGATGTTATGGAAAAAGCTGTTCAAGTT
ATTATAGAAATTGCAAGACGATTTGAAGAACAAGCTTAA

Upstream 100 bases:

>100_bases
ATAGCTAAAATACAAATGAAATATATGTATTCACAGTCAAAAAAGTTAAAATATTTCGTCTCAAGGATTATAATAGAGAA
AGAAGTATAAAAGGGAGTGA

Downstream 100 bases:

>100_bases
AAAAGAACCAAGTAGTGCAAAAACGCTACTTGGTTTTTTAGTATAATACTCGCCACAAATAAAAAGTTGCGTAAGACTCC
CAGTTGATCCAATTTGCCGC

Product: peptidase t

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNLKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEIGLTEVTMDDNGYVMATLPANTDKDVPVIGFLA
HLDTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLI
HNPQIKHGKIRVAFTPDEEIGRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNAT
KLAMEFNSYLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRENFEARKHNVENIVKQMQEKYGRDAVVLEMN
DQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQV
IIEIARRFEEQA

Sequences:

>Translated_412_residues
MNLKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEIGLTEVTMDDNGYVMATLPANTDKDVPVIGFLA
HLDTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLI
HNPQIKHGKIRVAFTPDEEIGRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNAT
KLAMEFNSYLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRENFEARKHNVENIVKQMQEKYGRDAVVLEMN
DQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQV
IIEIARRFEEQA
>Mature_412_residues
MNLKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEIGLTEVTMDDNGYVMATLPANTDKDVPVIGFLA
HLDTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLI
HNPQIKHGKIRVAFTPDEEIGRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNAT
KLAMEFNSYLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRENFEARKHNVENIVKQMQEKYGRDAVVLEMN
DQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQV
IIEIARRFEEQA

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=401, Percent_Identity=54.6134663341646, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_BACCQ (B9IV41)

Other databases:

- EMBL:   CP000227
- RefSeq:   YP_002531254.1
- MEROPS:   M20.003
- EnsemblBacteria:   EBBACT00000099765
- GeneID:   7375977
- GenomeReviews:   CP000227_GR
- KEGG:   bcq:BCQ_3537
- GeneTree:   EBGT00050000000947
- ProtClustDB:   PRK05469
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 46214; Mature: 46214

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 83-83 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEIGLTEVTMDDNGY
CCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCE
VMATLPANTDKDVPVIGFLAHLDTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQ
EEEEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEECCCCEEEECCHH
FPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHNPQIKHGKIRVAFTPDEEI
CCCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHH
GRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNAT
CCCCCCEEHHHCCCEEEEEECCCCCCCCEECCCCCCCCEEEECCCCCCCCCHHHHHHHHH
KLAMEFNSYLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRENFEARKHNVE
HHHHHHCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHCCHH
NIVKQMQEKYGRDAVVLEMNDQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGT
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
DGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVIIEIARRFEEQA
CCCCEEEECCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEIGLTEVTMDDNGY
CCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCE
VMATLPANTDKDVPVIGFLAHLDTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQ
EEEEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEECCCCEEEECCHH
FPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHNPQIKHGKIRVAFTPDEEI
CCCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHH
GRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNAT
CCCCCCEEHHHCCCEEEEEECCCCCCCCEECCCCCCCCEEEECCCCCCCCCHHHHHHHHH
KLAMEFNSYLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRENFEARKHNVE
HHHHHHCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHCCHH
NIVKQMQEKYGRDAVVLEMNDQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGT
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
DGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVIIEIARRFEEQA
CCCCEEEECCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA