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The map label for this gene is miaA [H]

Identifier: 222097169

GI number: 222097169

Start: 3460254

End: 3461207

Strand: Reverse

Name: miaA [H]

Synonym: BCQ_3509

Alternate gene names: 222097169

Gene position: 3461207-3460254 (Counterclockwise)

Preceding gene: 222097171

Following gene: 222097168

Centisome position: 66.38

GC content: 37.74

Gene sequence:

>954_bases
ATGGGAGAAGTGCAACGTGAAAAAGTTGCTGTCATCATTGGACCAACTGCTGTTGGGAAGACGAAGTTAAGTATTGATCT
TGCCAAAGCGTTAAATGGTGAAATTATAAGTGGAGATTCCATGCAGATTTATCGTACGATGGATATTGGAACCGCAAAGG
TGACGAAAGAAGAGATGGACGGAATTCCACATTATATGGTTGATATAAAAAATCCAGAAGAATCATTTTCGGTTGCCGAA
TTTCAAGAACGTGTTCGTAAGCATATTCGCGAGATTACAGAGCGTGGTAAATTGCCGATTATCGTTGGTGGAACTGGTCT
GTATATACAATCTGTTTTATTCGATTATCAGTTTACGGATGATGCTGGTGATGCTATATACCGAGAACAAATGGAAAAGT
TAGCATTAGAACGTGGTGTGGAATATGTACATAAGAAATTACAGGAAGTAGACCCAGAAAGCGCTGAGCGTATTCATGCA
AATAATGTACGCCGTGTCATTAGGGCGTTAGAAATTTTCCACACGACGGGTGAAAAAATGAGTGATCAACTTGAAAAACA
AGAAAACGAGTTATTATACGATGTTTCCTTAATTGGCTTGACAATGGATCGTGAAATGCTATACGATCGCATTAACTTAC
GAGTTGATATAATGATGGACCAAGGTTTATTAGAAGAAGTAGAAGGGTTGTATAATCGAGGGATACGAGATTGTCAATCT
ATTCAAGCGATTGGGTATAAAGAGATATATGATTATTTTGAGGATCGTGTCTCTTTAGAAGAAGCGGTATCACAATTAAA
GACGAATTCACGTCGTTATGCAAAACGTCAATTAACGTGGTTCCGTAATAAGATGGATGTCACATGGTTTGATGTTACAG
ACGGTGAAAAAACGTCAGAAATTTTACGATACATAGAAGGAAAGCTACAACTAAAGTCGAATAATAGTAAGTAG

Upstream 100 bases:

>100_bases
CATGTACCGTAATGAAAGAACTTGCAATCATGCCGTTAAAATTGGTAAGATTGGATATAATCAATAGATGGAGTACATTA
CATAAAGAGAGTTGAATGAT

Downstream 100 bases:

>100_bases
AGAAAAAGAGGAGGATTCGACATGAAGCAATCAATCAATATTCAAGATCAGTTTTTAAATCAACTCCGTAAAGAGAATAC
GTTTGTTACGCTGTACTTAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHTTGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Sequences:

>Translated_317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHTTGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK
>Mature_316_residues
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAEF
QERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHAN
NVRRVIRALEIFHTTGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSI
QAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=32.1656050955414, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=38.0794701986755, Blast_Score=215, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI25144712, Length=307, Percent_Identity=32.8990228013029, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=34.5276872964169, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24649830, Length=341, Percent_Identity=31.6715542521994, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36666; Mature: 36535

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMD
CCCCCCCCEEEEECCCCCCCEEHHHHHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHC
GIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTTGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
SDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHEEEEHHHHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYIEGKLQLKSNNSK
HHHHHCCCEEEECCCCC
>Mature Secondary Structure 
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMD
CCCCCCCEEEEECCCCCCCEEHHHHHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHC
GIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTTGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
SDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHEEEEHHHHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYIEGKLQLKSNNSK
HHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA