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The map label for this gene is cwlH [H]

Identifier: 222097138

GI number: 222097138

Start: 3429174

End: 3430901

Strand: Reverse

Name: cwlH [H]

Synonym: BCQ_3478

Alternate gene names: 222097138

Gene position: 3430901-3429174 (Counterclockwise)

Preceding gene: 222097139

Following gene: 222097137

Centisome position: 65.8

GC content: 35.59

Gene sequence:

>1728_bases
ATGAAAAAAGGATTTTATAATGTTTTAGCTGCATCAATTGTATTTAGTATGGTAACGATTCCAAATTATTCCTATGCTAA
TGAACTTGGAAAAACTGTTACAGTTTCATCTGAAGAACAAGCTTTAAAAAGTATTGAAAACCATATGAAAGATGAAGATG
GTCGAGGGGAAGATAAAGGAGTAAGAAGTGAAGTGCAAGGTGAGTTTCTTGTACATATAGTGAAAGAAGTACCTTTATAC
GATTCTAGTAATTTTCAAAAAGAGACAGGGATTCGTATTTCGAATCAAGTCGTAAAAGCTGAGAAGAGAAAAGGAAACGC
TTACTATGTTCAAACTTCATCGGGAACGGGATGGATACAAAATAGTGATGGAAATGTAGAGGTTAAAGAAATTCACCCGT
TATTAAGTGAAAAGTTAGTTATTAATGAAGAAAGCTCTACTTACTCTGAACCATTTACTTCATACAAAGAAGAAAATGTA
CTGGAGCCGCAAACTATTCAAGCAATTGGACAGGCGGGAGAATGGTTCCAAGTAAAAATAAATAATGAGATGAAATGGAT
TCATTCACCTTCAGCAAAATTTGAAGGAATAAAAGCTTCACTTATACAAGATGCAGCACCAACACGTACAAAACGTGCAG
CAGCTATGTATGCTGCGCCAATAGAAGAGAAAACAACGGATATTTATGGTGTACCTTTAAAAGAAATGATTGTACCAAAA
GGAAATGAAAATATTCGTCCAGGTTATGCGATGAACCCGAAGTATATTACGATTCATGAGACCGATAATTATAATGTTGG
GGCAAATGCTAGAAACCATGCGATATATTTATATAACCAAGCAACGGGAACGGAAGATCGTTCAGCGTCGTGGCACTTTA
CGGTAGATGATAAAGAGATTTATCAGCATCTCCCACTAAATGAAAATGCTTGGCATGCTGGAGATGGAGCTGAAGGAACT
GGGAATCGAGAATCAATTGCGATTGAAATTGCTGTAAACGAAGATGGTGATTATAATAAAGCTGTTGAAAATGCTCGTAA
ACTAGCAGCTTATTTAATGGGAGAATTAAATATTCCTTTAGAAAATGTGAAAAAACATCAATTTTGGAGTGGGAAGATTT
GTCCAGCCATTATGATTAAAAATAATGGGTGGGAACCATTCTTGCAAGGAACGAAAGTTTATTATGATGCGAACCATAAA
GATGATATAACGGGTGGTTGGTATGAAGCAGACATTCGTGAATTAGATAAGCGCGGTATTATGGTTGGAGATGGAAAAGG
TTCTTATTGGCCAGAGCGCCTTGTGACACGTGGTGAATTTGCAAATTTAATATCAAGATCATTGCAGCTACCTGAAGGAT
CATCGAATTTTAATGACTTAAATACAGCACATCCATCGCTAATAGATGGTATTAATCGTGCAGCAAGTGCTGGGATAATA
AGTGGTAGAGGAAATGGTATATTTGCTCCAAACGATACGATTAAACGAGATGAAGCTGTTATCATGATTGACCGTGCACT
GCAATATAAAAAAATTAAAGGTAACTTAGTCTCGTTACCATTTTCAGATCAAGATTTAGCATATGATAAACAAGCTGTAC
AACGTGTTTATGGATTAGGTATTGTAAAAGGAAATGAAAATAATGAATTTATGCCGAAAGGTTCTGCAACGCGTGGAGAA
TCGGCAGCATTTATTAATAGAATGTTAAAAGTAATAGAAAGTAAATAA

Upstream 100 bases:

>100_bases
TAAATGGTTATGTTGCTTAATAGTTTAGTAGATAATCTATATTTTGGTTGAGGAGGGGAATTTTAACACGAATTTAATGA
TATGCGTGTTAAGTAGTATG

Downstream 100 bases:

>100_bases
TGAAAAAGCATTCGCGATTGCGAGTGCTTTTTTATTTTTCAATACGTTACGGAAGGGAGAGAAGGGTTTTGAAGGGAATA
AGGAATGAAAGCGGAGGTGA

Product: N-acetylmuramoyl-l-alanine amidase, family 2

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKKGFYNVLAASIVFSMVTIPNYSYANELGKTVTVSSEEQALKSIENHMKDEDGRGEDKGVRSEVQGEFLVHIVKEVPLY
DSSNFQKETGIRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLVINEESSTYSEPFTSYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGIKASLIQDAAPTRTKRAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYNVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHK
DDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANLISRSLQLPEGSSNFNDLNTAHPSLIDGINRAASAGII
SGRGNGIFAPNDTIKRDEAVIMIDRALQYKKIKGNLVSLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGE
SAAFINRMLKVIESK

Sequences:

>Translated_575_residues
MKKGFYNVLAASIVFSMVTIPNYSYANELGKTVTVSSEEQALKSIENHMKDEDGRGEDKGVRSEVQGEFLVHIVKEVPLY
DSSNFQKETGIRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLVINEESSTYSEPFTSYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGIKASLIQDAAPTRTKRAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYNVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHK
DDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANLISRSLQLPEGSSNFNDLNTAHPSLIDGINRAASAGII
SGRGNGIFAPNDTIKRDEAVIMIDRALQYKKIKGNLVSLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGE
SAAFINRMLKVIESK
>Mature_575_residues
MKKGFYNVLAASIVFSMVTIPNYSYANELGKTVTVSSEEQALKSIENHMKDEDGRGEDKGVRSEVQGEFLVHIVKEVPLY
DSSNFQKETGIRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLVINEESSTYSEPFTSYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGIKASLIQDAAPTRTKRAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYNVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHK
DDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANLISRSLQLPEGSSNFNDLNTAHPSLIDGINRAASAGII
SGRGNGIFAPNDTIKRDEAVIMIDRALQYKKIKGNLVSLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGE
SAAFINRMLKVIESK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 64083; Mature: 64083

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGFYNVLAASIVFSMVTIPNYSYANELGKTVTVSSEEQALKSIENHMKDEDGRGEDKG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
VRSEVQGEFLVHIVKEVPLYDSSNFQKETGIRISNQVVKAEKRKGNAYYVQTSSGTGWIQ
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEHHHHHHHHHCCCCEEEEEECCCCCEEE
NSDGNVEVKEIHPLLSEKLVINEESSTYSEPFTSYKEENVLEPQTIQAIGQAGEWFQVKI
CCCCCEEHHHHHHHHHCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
NNEMKWIHSPSAKFEGIKASLIQDAAPTRTKRAAAMYAAPIEEKTTDIYGVPLKEMIVPK
CCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCHHHHCCCC
GNENIRPGYAMNPKYITIHETDNYNVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEI
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECHHHH
YQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCH
ENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGI
HHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCE
MVGDGKGSYWPERLVTRGEFANLISRSLQLPEGSSNFNDLNTAHPSLIDGINRAASAGII
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEE
SGRGNGIFAPNDTIKRDEAVIMIDRALQYKKIKGNLVSLPFSDQDLAYDKQAVQRVYGLG
ECCCCEEECCCCCCCCCCEEEEEECHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCE
IVKGNENNEFMPKGSATRGESAAFINRMLKVIESK
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKGFYNVLAASIVFSMVTIPNYSYANELGKTVTVSSEEQALKSIENHMKDEDGRGEDKG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
VRSEVQGEFLVHIVKEVPLYDSSNFQKETGIRISNQVVKAEKRKGNAYYVQTSSGTGWIQ
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEHHHHHHHHHCCCCEEEEEECCCCCEEE
NSDGNVEVKEIHPLLSEKLVINEESSTYSEPFTSYKEENVLEPQTIQAIGQAGEWFQVKI
CCCCCEEHHHHHHHHHCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
NNEMKWIHSPSAKFEGIKASLIQDAAPTRTKRAAAMYAAPIEEKTTDIYGVPLKEMIVPK
CCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCHHHHCCCC
GNENIRPGYAMNPKYITIHETDNYNVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEI
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECHHHH
YQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCH
ENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGI
HHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCE
MVGDGKGSYWPERLVTRGEFANLISRSLQLPEGSSNFNDLNTAHPSLIDGINRAASAGII
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEE
SGRGNGIFAPNDTIKRDEAVIMIDRALQYKKIKGNLVSLPFSDQDLAYDKQAVQRVYGLG
ECCCCEEECCCCCCCCCCEEEEEECHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCE
IVKGNENNEFMPKGSATRGESAAFINRMLKVIESK
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]