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The map label for this gene is yhfS [H]

Identifier: 222097084

GI number: 222097084

Start: 3370650

End: 3371741

Strand: Reverse

Name: yhfS [H]

Synonym: BCQ_3424

Alternate gene names: 222097084

Gene position: 3371741-3370650 (Counterclockwise)

Preceding gene: 222097085

Following gene: 222097083

Centisome position: 64.66

GC content: 40.2

Gene sequence:

>1092_bases
ATGAATAGAGCGGTTATTGTAGAGGCGAAAAGAACTCCTATCGGTAAGAAGAATGGGATGTTAAAAAACTATGAAGTTCA
GCAATTAGCAACGCCACTTCTTACATTTTTAAGTAAAGGAATGGAGAGAGAAATAGATGATGTCATATTAGGGAATGTTG
TTGGGCCAGGAGGGAATGTTGCGAGATTATCTGCTTTAGAAGCAGGGCTTAGTCATCATATTCCGGGTGTAACGATTGAC
CGACAATGTGGTGCCGGATTGGAAGCGATTCGCACCGCATGTCATTTTATTCAAGGCGGAGCAGGTAATTGTTATATTGC
AGGAGGAGTAGAGAGTACAAGTACGTCACCTTTTCAAAATAGAGCACGATTCTCACCAGAAACAATAGGCGATCCTAATA
TGGGAGTAGCAGCTGAATATGTTGCAGAACGTTATAACATCACGAGAGAAATGCAAGACGAGTATGCTTGCCTCAGCTAC
AAACGAACACTGCAAGCGTTAGAAAAAGGATATATACATGATGAAATATTGTCTTTTAATGGATTGTTAGATGAGTCTAT
AAAGCCAGAAATGAATTATGAACGAATGATTAAAAGAACTAAACCCGCATTTCTACAAAATGGTACAGTAACGGCAGGGA
ATTCATGTGGTGTAAACGACGGGGCGTGTGCAGTTCTTGTAATGGAAGAGCGGCAAGCTCGAAAATTAGGATACAAGCCT
ATTCTTCGTTTTGTTTGTAGTGCTGTAGTTGGAGTAGATCCCAACCTTCCGGGGACTGGTCCAATATTTGCGGTGAACAA
ATTACTAAACGAAATGAATATGAAAGTAGAGGATATCGATTATTTTGAAATAAATGAAGCATTTGCTTCAAAAGTTGTAG
CTTGTGCAAAGACGTTACAAATTCCGTACGAGAAATTAAATGTAAATGGTGGTGCAATTGCGCTCGGTCATCCGTACGGT
GCATCTGGAGCTATGCTTGTAACACGTTTGTTTTATCAAGCGCAAAGAGAGCATATGAAGTATGGAATTACGACGTTAGG
AATAGGGGGCGGGATAGGGCTTGCTTTATTATTTGAGAAAGTAGAAGATTAG

Upstream 100 bases:

>100_bases
CGAAAGAATGGTATTTCGTTGATGAAATACCGTATACAAATAGCGGAAAAAATCGCTCGTATGGCAGCAAAAAGTATAAT
TGAAAACCGGGAGAAGATAT

Downstream 100 bases:

>100_bases
CGAGGTATTAACTGTACTATGATTAATAGGGGATAATTAGTTCAATATATGAGTTAATATCCGCTCTTAGTAACTATAAA
ACAAACAAATATTATTCTAT

Product: acetyl-CoA acetyltransferase

Products: CoA; acetoacetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNRAVIVEAKRTPIGKKNGMLKNYEVQQLATPLLTFLSKGMEREIDDVILGNVVGPGGNVARLSALEAGLSHHIPGVTID
RQCGAGLEAIRTACHFIQGGAGNCYIAGGVESTSTSPFQNRARFSPETIGDPNMGVAAEYVAERYNITREMQDEYACLSY
KRTLQALEKGYIHDEILSFNGLLDESIKPEMNYERMIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEERQARKLGYKP
ILRFVCSAVVGVDPNLPGTGPIFAVNKLLNEMNMKVEDIDYFEINEAFASKVVACAKTLQIPYEKLNVNGGAIALGHPYG
ASGAMLVTRLFYQAQREHMKYGITTLGIGGGIGLALLFEKVED

Sequences:

>Translated_363_residues
MNRAVIVEAKRTPIGKKNGMLKNYEVQQLATPLLTFLSKGMEREIDDVILGNVVGPGGNVARLSALEAGLSHHIPGVTID
RQCGAGLEAIRTACHFIQGGAGNCYIAGGVESTSTSPFQNRARFSPETIGDPNMGVAAEYVAERYNITREMQDEYACLSY
KRTLQALEKGYIHDEILSFNGLLDESIKPEMNYERMIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEERQARKLGYKP
ILRFVCSAVVGVDPNLPGTGPIFAVNKLLNEMNMKVEDIDYFEINEAFASKVVACAKTLQIPYEKLNVNGGAIALGHPYG
ASGAMLVTRLFYQAQREHMKYGITTLGIGGGIGLALLFEKVED
>Mature_363_residues
MNRAVIVEAKRTPIGKKNGMLKNYEVQQLATPLLTFLSKGMEREIDDVILGNVVGPGGNVARLSALEAGLSHHIPGVTID
RQCGAGLEAIRTACHFIQGGAGNCYIAGGVESTSTSPFQNRARFSPETIGDPNMGVAAEYVAERYNITREMQDEYACLSY
KRTLQALEKGYIHDEILSFNGLLDESIKPEMNYERMIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEERQARKLGYKP
ILRFVCSAVVGVDPNLPGTGPIFAVNKLLNEMNMKVEDIDYFEINEAFASKVVACAKTLQIPYEKLNVNGGAIALGHPYG
ASGAMLVTRLFYQAQREHMKYGITTLGIGGGIGLALLFEKVED

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=35.73264781491, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI167614485, Length=387, Percent_Identity=33.3333333333333, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4501853, Length=384, Percent_Identity=35.4166666666667, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI4557237, Length=391, Percent_Identity=32.9923273657289, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI4504327, Length=419, Percent_Identity=30.5489260143198, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194353979, Length=364, Percent_Identity=28.2967032967033, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI48994986, Length=389, Percent_Identity=38.3033419023136, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=37.8109452736318, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=38.9447236180905, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87082165, Length=396, Percent_Identity=36.1111111111111, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=26.8292682926829, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI133906874, Length=392, Percent_Identity=37.2448979591837, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=30.1546391752577, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=32.0610687022901, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=29.3144208037825, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=377, Percent_Identity=26.790450928382, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322031, Length=387, Percent_Identity=34.625322997416, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6325229, Length=392, Percent_Identity=32.3979591836735, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24655093, Length=394, Percent_Identity=38.0710659898477, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17648125, Length=390, Percent_Identity=37.6923076923077, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=32.133676092545, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI17137578, Length=418, Percent_Identity=29.4258373205742, Blast_Score=158, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9

Molecular weight: Translated: 39391; Mature: 39391

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00737 THIOLASE_2 ; PS00139 THIOL_PROTEASE_CYS ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRAVIVEAKRTPIGKKNGMLKNYEVQQLATPLLTFLSKGMEREIDDVILGNVVGPGGNV
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
ARLSALEAGLSHHIPGVTIDRQCGAGLEAIRTACHFIQGGAGNCYIAGGVESTSTSPFQN
HHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHH
RARFSPETIGDPNMGVAAEYVAERYNITREMQDEYACLSYKRTLQALEKGYIHDEILSFN
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
GLLDESIKPEMNYERMIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEERQARKLGYKP
CCCCCCCCCCCCHHHHHHHCCCHHCCCCCEECCCCCCCCCCCEEEEEECCHHHHHCCHHH
ILRFVCSAVVGVDPNLPGTGPIFAVNKLLNEMNMKVEDIDYFEINEAFASKVVACAKTLQ
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEHHHHHHHHHHHHHHHHH
IPYEKLNVNGGAIALGHPYGASGAMLVTRLFYQAQREHMKYGITTLGIGGGIGLALLFEK
CCHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHH
VED
HCC
>Mature Secondary Structure
MNRAVIVEAKRTPIGKKNGMLKNYEVQQLATPLLTFLSKGMEREIDDVILGNVVGPGGNV
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
ARLSALEAGLSHHIPGVTIDRQCGAGLEAIRTACHFIQGGAGNCYIAGGVESTSTSPFQN
HHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHH
RARFSPETIGDPNMGVAAEYVAERYNITREMQDEYACLSYKRTLQALEKGYIHDEILSFN
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
GLLDESIKPEMNYERMIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEERQARKLGYKP
CCCCCCCCCCCCHHHHHHHCCCHHCCCCCEECCCCCCCCCCCEEEEEECCHHHHHCCHHH
ILRFVCSAVVGVDPNLPGTGPIFAVNKLLNEMNMKVEDIDYFEINEAFASKVVACAKTLQ
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEHHHHHHHHHHHHHHHHH
IPYEKLNVNGGAIALGHPYGASGAMLVTRLFYQAQREHMKYGITTLGIGGGIGLALLFEK
CCHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHH
VED
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA

Specific reaction: 2 acetyl-CoA = CoA + acetoacetyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]