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The map label for this gene is yxeM [H]

Identifier: 222096981

GI number: 222096981

Start: 3264290

End: 3265081

Strand: Reverse

Name: yxeM [H]

Synonym: BCQ_3321

Alternate gene names: 222096981

Gene position: 3265081-3264290 (Counterclockwise)

Preceding gene: 222096982

Following gene: 222096980

Centisome position: 62.62

GC content: 33.84

Gene sequence:

>792_bases
ATGAGGAAATCATCATTATTACTAACATTGGCATTAACAGTATCGGTTGGGCTTTTATCTGCTTGTGGTTCAGATACAGC
GAGCAAAAAAACAGATGAAAAAGTAGTGAAAGTTGGGACGTCTGCAGGTCGTAGTCCATTTATTTTTAAAGAGGGTGAGA
AAGTAAAAGGCTTTGATGCTGAAATTATTGAAGCGGCTGCTAAAAAAGCTGGATATAAAGTTGAATGGAACGTTTCTGAT
TTTGAAGGATTATTCGGTTTATTAGACTCGGGTCGTATTGATACAATTGCAAATGAATTATCGGTTTCTCCGGAAAGAAA
AAAGAAATATGACTTTTCAATTCCATATGTTTATTCAGGATCTGTTTTCGCGGTGAAGAAAGATAATAATACAATTAAAT
CGATAGAAGATCTAAAAGGAAAGACTGTAGGTGTGGGGCTTGGTACAGCCGGGGAACAAGAATTAAAAGCTTTAAATAAA
AATAATGCTTTTACAATTAAAACGTTCTCAGAAGATCCAACAGCAGAATTGAATGAAGTGGGACTTGGTCGTGTAGATGC
TTACTATAATGATAAAGTGCAAGTAGAAACAACAATTAAAAAGGCAGATTTAGATAATGTGAAAGTAGGATTTGGTCCAC
TTAAATGGGGAGAGATTGCATTTCCATTTGCTAAAAAGAGCGAAAAGTTAGAAGATATAAATAAAGCATTGAAAGAATTA
GAACAAGATGGGACATTAAGCGAAATATCAAAAAAATGGCTGAAAGTTGATGCTACAAAAAAACAAAATTAA

Upstream 100 bases:

>100_bases
AAATTTTTCGGAATCCACAAAATGAGCGTACGAAAAAGTTTTTACGACAATTGAATGCTTCAGAAGAAGGCAATCATTTC
GTTATTTAGGAGGAAAAATT

Downstream 100 bases:

>100_bases
AGTATGTTCGTTGAATGTGGCTATTGTCCATCAACTTATATAAAAATAAAAGGAAATTGGGGAGGAAGTTACTCATGGTA
CAAATTCATCCAAAAACACG

Product: periplasmic amino acid binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRKSSLLLTLALTVSVGLLSACGSDTASKKTDEKVVKVGTSAGRSPFIFKEGEKVKGFDAEIIEAAAKKAGYKVEWNVSD
FEGLFGLLDSGRIDTIANELSVSPERKKKYDFSIPYVYSGSVFAVKKDNNTIKSIEDLKGKTVGVGLGTAGEQELKALNK
NNAFTIKTFSEDPTAELNEVGLGRVDAYYNDKVQVETTIKKADLDNVKVGFGPLKWGEIAFPFAKKSEKLEDINKALKEL
EQDGTLSEISKKWLKVDATKKQN

Sequences:

>Translated_263_residues
MRKSSLLLTLALTVSVGLLSACGSDTASKKTDEKVVKVGTSAGRSPFIFKEGEKVKGFDAEIIEAAAKKAGYKVEWNVSD
FEGLFGLLDSGRIDTIANELSVSPERKKKYDFSIPYVYSGSVFAVKKDNNTIKSIEDLKGKTVGVGLGTAGEQELKALNK
NNAFTIKTFSEDPTAELNEVGLGRVDAYYNDKVQVETTIKKADLDNVKVGFGPLKWGEIAFPFAKKSEKLEDINKALKEL
EQDGTLSEISKKWLKVDATKKQN
>Mature_263_residues
MRKSSLLLTLALTVSVGLLSACGSDTASKKTDEKVVKVGTSAGRSPFIFKEGEKVKGFDAEIIEAAAKKAGYKVEWNVSD
FEGLFGLLDSGRIDTIANELSVSPERKKKYDFSIPYVYSGSVFAVKKDNNTIKSIEDLKGKTVGVGLGTAGEQELKALNK
NNAFTIKTFSEDPTAELNEVGLGRVDAYYNDKVQVETTIKKADLDNVKVGFGPLKWGEIAFPFAKKSEKLEDINKALKEL
EQDGTLSEISKKWLKVDATKKQN

Specific function: Probably part of the ABC transporter complex yxeMNO that could be involved in amino-acid import. May transport S- methylcysteine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=257, Percent_Identity=34.6303501945525, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787031, Length=255, Percent_Identity=34.5098039215686, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788649, Length=262, Percent_Identity=27.8625954198473, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787085, Length=230, Percent_Identity=26.5217391304348, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=23.9043824701195, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=26.7241379310345, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28717; Mature: 28717

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSSLLLTLALTVSVGLLSACGSDTASKKTDEKVVKVGTSAGRSPFIFKEGEKVKGFDA
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCEEEECCCCCCCCCH
EIIEAAAKKAGYKVEWNVSDFEGLFGLLDSGRIDTIANELSVSPERKKKYDFSIPYVYSG
HHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCCCCEEECC
SVFAVKKDNNTIKSIEDLKGKTVGVGLGTAGEQELKALNKNNAFTIKTFSEDPTAELNEV
CEEEEECCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHC
GLGRVDAYYNDKVQVETTIKKADLDNVKVGFGPLKWGEIAFPFAKKSEKLEDINKALKEL
CCCEEEEEECCEEEEEEEEECCCCCCEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHH
EQDGTLSEISKKWLKVDATKKQN
HCCCCHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MRKSSLLLTLALTVSVGLLSACGSDTASKKTDEKVVKVGTSAGRSPFIFKEGEKVKGFDA
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCEEEECCCCCCCCCH
EIIEAAAKKAGYKVEWNVSDFEGLFGLLDSGRIDTIANELSVSPERKKKYDFSIPYVYSG
HHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCCCCEEECC
SVFAVKKDNNTIKSIEDLKGKTVGVGLGTAGEQELKALNKNNAFTIKTFSEDPTAELNEV
CEEEEECCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHC
GLGRVDAYYNDKVQVETTIKKADLDNVKVGFGPLKWGEIAFPFAKKSEKLEDINKALKEL
CCCEEEEEECCEEEEEEEEECCCCCCEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHH
EQDGTLSEISKKWLKVDATKKQN
HCCCCHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]