BacMap

Definition	Bacillus cereus Q1 chromosome, complete genome.
Accession	NC_011969
Length	5,214,195

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The map label for this gene is ytbE [H]

Identifier: 222096856

GI number: 222096856

Start: 3148886

End: 3149725

Strand: Reverse

Name: ytbE [H]

Synonym: BCQ_3196

Alternate gene names: 222096856

Gene position: 3149725-3148886 (Counterclockwise)

Preceding gene: 222096857

Following gene: 222096855

Centisome position: 60.41

GC content: 35.6

Gene sequence:

>840_bases
ATGAAAAACTTACAAAGTACAACAACATTGCATAACGGTGTAGAAATGCCTTGGTTTGGTTTAGGTGTATTTAAAGTAGA
AGAAGGACCAGAGCTTGTAGAAGCAATTAAATCAGCAATTAAGGCAGGATACCGTAGCATCGATACAGCTGCGATTTATG
GAAATGAAGCAGCTGTAGGTGAAGGAATTCGCGCTGGTATTGAAGCGACTGGAATTTCAAGAGAAGAATTATTTATCACT
TCAAAAGTATGGAACGCAGATCAAGGATACGAAGAAACAATCGCTGCATATGAAGAGAGTTTAAAAAAATTAAAACTAGA
TTATTTAGATTTATATCTTGTTCACTGGCCTGTAGAAGGGAAATATAAAGATACGTGGAGAGCGTTAGAAACGCTTTATA
AAGAAAAGCGTGTACGTGCAATTGGCGTAAGTAACTTCCAAATTCATCATTTGCAAGATGTAATAAAAGATGCAGAAATT
AAGCCAATGATCAACCAAGTAGAATACCATCCTCGTTTAACACAAAAAGAATTACAAGCTTTCTGTAAAGAACAAGGTAT
TCAAATGGAAGCATGGTCACCACTAATGCAAGGTCAATTATTAGATAATGAAACATTACAGGCAATTGCTGAGAAATACG
GTAAAACAACAGCACAAGTGATTTTACGTTGGGATCTTCAAAATGGAGTAATTACAATTCCAAAATCAACGAAAGAACAC
CGCATTATTGCAAACGCTGATGTATTTAACTTTGAATTAACGAAAGAAGATATGGAAAAAATCGATGCGTTAAACCAAAA
TCACCGCGTGGGTCCAGACCCTGATAACTTTGATTTCTAA

Upstream 100 bases:

>100_bases
TGGGTAGGCGGCATTATGGTAGTAGGTGCTGTTATTTTAACAGCATGGAGCATGTCATTAGAAAAACGAGATCAAGTAAA
ATAAAAGGAGAGAAAATATA

Downstream 100 bases:

>100_bases
GATTAAAGAATGAGTAAAAAGAGTGCAAAGTTGCACTCTTTTTTATTTTCTATTCAATAAAATGTTTCTGTTTATTTGAT
TAGCGTTTATATTATGGCTG

Product: oxidoreductase, aldo/keto reductase family

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYEETIAAYEESLKKLKLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKYGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Sequences:

>Translated_279_residues
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYEETIAAYEESLKKLKLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKYGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
>Mature_279_residues
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYEETIAAYEESLKKLKLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKYGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=40.3389830508475, Blast_Score=230, Evalue=9e-61,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=40.3389830508475, Blast_Score=230, Evalue=9e-61,
Organism=Homo sapiens, GI223468663, Length=292, Percent_Identity=39.7260273972603, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=37.0491803278689, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI310109922, Length=300, Percent_Identity=38.6666666666667, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI310109920, Length=320, Percent_Identity=37.8125, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI45446745, Length=320, Percent_Identity=37.5, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI4503285, Length=320, Percent_Identity=37.5, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI5453543, Length=319, Percent_Identity=37.3040752351097, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=38.6440677966102, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI291291012, Length=278, Percent_Identity=38.8489208633094, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI24497583, Length=302, Percent_Identity=36.4238410596026, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI4502049, Length=299, Percent_Identity=38.7959866220736, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=35.472972972973, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.1818181818182, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI300116271, Length=274, Percent_Identity=33.9416058394161, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI310109924, Length=160, Percent_Identity=38.75, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI310109926, Length=123, Percent_Identity=43.0894308943089, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI207028673, Length=123, Percent_Identity=42.2764227642276, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI87082198, Length=276, Percent_Identity=45.2898550724638, Blast_Score=250, Evalue=7e-68,
Organism=Escherichia coli, GI1786400, Length=263, Percent_Identity=37.2623574144487, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788081, Length=274, Percent_Identity=32.4817518248175, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788070, Length=325, Percent_Identity=24, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081735, Length=282, Percent_Identity=23.4042553191489, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=273, Percent_Identity=41.7582417582418, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=36.4548494983278, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=39.6610169491525, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17537075, Length=304, Percent_Identity=38.8157894736842, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=37, Blast_Score=182, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=33.6996336996337, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17537079, Length=312, Percent_Identity=33.3333333333333, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=35.0553505535055, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=36.5313653136531, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=32.4817518248175, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=35.0553505535055, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17566692, Length=287, Percent_Identity=33.4494773519164, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=36.9085173501577, Blast_Score=199, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320576, Length=300, Percent_Identity=36, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=35.7692307692308, Blast_Score=181, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6319625, Length=297, Percent_Identity=37.037037037037, Blast_Score=179, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324694, Length=290, Percent_Identity=36.2068965517241, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6320079, Length=291, Percent_Identity=34.7079037800687, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24663317, Length=298, Percent_Identity=40.2684563758389, Blast_Score=213, Evalue=9e-56,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=39.4557823129252, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=37.6666666666667, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24657054, Length=291, Percent_Identity=39.1752577319588, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI20129731, Length=296, Percent_Identity=37.1621621621622, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=35.3125, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45553081, Length=295, Percent_Identity=33.5593220338983, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24662785, Length=291, Percent_Identity=41.5807560137457, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24662781, Length=293, Percent_Identity=41.296928327645, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=35.0515463917526, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=33.59375, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31799; Mature: 31799

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVG
CCCCCCHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHC
EGIRAGIEATGISREELFITSKVWNADQGYEETIAAYEESLKKLKLDYLDLYLVHWPVEG
CHHHCCCHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKYGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVG
CCCCCCHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHC
EGIRAGIEATGISREELFITSKVWNADQGYEETIAAYEESLKKLKLDYLDLYLVHWPVEG
CHHHCCCHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKYGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]