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The map label for this gene is pykA [H]

Identifier: 222096797

GI number: 222096797

Start: 3090857

End: 3091915

Strand: Reverse

Name: pykA [H]

Synonym: BCQ_3137

Alternate gene names: 222096797

Gene position: 3091915-3090857 (Counterclockwise)

Preceding gene: 222096798

Following gene: 222096794

Centisome position: 59.3

GC content: 37.2

Gene sequence:

>1059_bases
GTGACAATCGATCGAATTTGTACAATTGGTCCAGCAAGTAATAATAAAGAAACGTTAGCGCAGTTAATAAGCAATGGTAT
GAAAATTGTTCGGCTAAATTTATCACATGGGACGCATGAAAGTCATAAAGATATCATTCGTTTAGTGAAATCATTAGATG
ATTCTATTAAAATTTTAGGCGATGTACAAGGTCCTAAAATAAGGTTAGGTGAAATGAAAGGAGAACAAATTACACTTCAG
GCGGGAGATTCTTTTATTTTGCATACGCAACCAGTTACAGGGAGTAGTACGGAAGCAAGTGTTGATTATGAAGGGATCGC
GAATGATGTGAAAGTCGGAAGTAGAATTTTAATGAATGATGGTGAAGTTGAGTTAATCGTTGAGAAAGTAAGTACGGATA
AAATAGAAACGAAGGTGAAAACAGGTGGTAATATCTCCTCTCATAAAGGGGTGAACTTACCAGGCGCAATCGTTCGTTTA
CCAGCTATTACAGAGAAAGATAAAAAAGATATTCAGTTTCTTTTAGAAGAAGGTGTTGATTTTATCGCATGTTCTTTCGT
AAGAAAACCTAGCCATATAAAAGAAATACGAGATTTTATACAACAGTATAAAGAAACGTCACCTAATTTAATTGCAAAAA
TAGAAACGATGGAAGCGATCGAGAATTTTCAAGATATATGTAAAGAAGCAGATGGAATCATGATTGCAAGGGGAGATTTA
GGAGTTGAGTTACCGTTTCAATTTATTCCGCTCTTGCAAAAAATGATGATTCAGGAATGCAATCGAACGAATACATATGT
TATTACAGCGACACAAATGCTTCAATCTATGGTAGATCATTCTATTCCAACAAGGGCAGAGGTAACGGATGTGTTCCAAG
CTGTACTGGACGGGACGAATGCCGTTATGCTTTCTGCTGAAAGTGCTTCAGGAGAGCATCCGGTTGAAAGTGTGAGTACA
CTACGTCTCGTTTCTGAATTTGCTGAGCATGTGAAAAAAGACGGTCCTTTTGCGATGAAAGATGTACTGGAATTGCTGCA
TAAGTCTTTGGATGGGTGA

Upstream 100 bases:

>100_bases
TTCGTAGTTTCAAAAGCCTACTTGTGTGAGTAGGCTTTTTTTACGTTGAGAGTGTAATGAAGGTTTATGGGAATCATAAT
GAAAAAGGATGGTGATAAAA

Downstream 100 bases:

>100_bases
TGAATAAAAAGAGGACGGATATGTATCCGTCCTCGTGTTTTTCATTCATTCATTCTACTCTTGCTCTTGCTCATCCAAAA
TCTTTTCAAACGCAGCAGAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEMKGEQITLQ
AGDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVRL
PAITEKDKKDIQFLLEEGVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVST
LRLVSEFAEHVKKDGPFAMKDVLELLHKSLDG

Sequences:

>Translated_352_residues
MTIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEMKGEQITLQ
AGDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVRL
PAITEKDKKDIQFLLEEGVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVST
LRLVSEFAEHVKKDGPFAMKDVLELLHKSLDG
>Mature_351_residues
TIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEMKGEQITLQA
GDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVRLP
AITEKDKKDIQFLLEEGVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDLG
VELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVSTL
RLVSEFAEHVKKDGPFAMKDVLELLHKSLDG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=340, Percent_Identity=42.3529411764706, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI33286420, Length=340, Percent_Identity=42.3529411764706, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI33286418, Length=340, Percent_Identity=42.3529411764706, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI10835121, Length=340, Percent_Identity=41.4705882352941, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI32967597, Length=340, Percent_Identity=41.4705882352941, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=40.6360424028269, Blast_Score=207, Evalue=8e-54,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=40.6360424028269, Blast_Score=207, Evalue=8e-54,
Organism=Homo sapiens, GI310128736, Length=231, Percent_Identity=38.0952380952381, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI310128734, Length=231, Percent_Identity=38.0952380952381, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=35.8139534883721, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1787965, Length=332, Percent_Identity=43.3734939759036, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788160, Length=335, Percent_Identity=40, Blast_Score=231, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI71984413, Length=336, Percent_Identity=46.7261904761905, Blast_Score=280, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=336, Percent_Identity=46.7261904761905, Blast_Score=280, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI71984406, Length=336, Percent_Identity=46.7261904761905, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=336, Percent_Identity=46.7261904761905, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=350, Percent_Identity=41.4285714285714, Blast_Score=263, Evalue=9e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=336, Percent_Identity=38.9880952380952, Blast_Score=225, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6324923, Length=334, Percent_Identity=36.8263473053892, Blast_Score=202, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=42.9411764705882, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=42.9411764705882, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24648966, Length=322, Percent_Identity=39.7515527950311, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24581235, Length=339, Percent_Identity=34.5132743362832, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24646914, Length=182, Percent_Identity=39.5604395604396, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 38723; Mature: 38592

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILG
CCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHEEHC
DVQGPKIRLGEMKGEQITLQAGDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRILMND
CCCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCHHHHHHCCEEEECC
GEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVRLPAITEKDKKDIQFLLEEGVD
CCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHCCCH
FIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPVESVSTLRLVSEFAEHVKKDGPFAMKDVLELLHKSLDG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILG
CCCCEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHEEHC
DVQGPKIRLGEMKGEQITLQAGDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRILMND
CCCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCHHHHHHCCEEEECC
GEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVRLPAITEKDKKDIQFLLEEGVD
CCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHCCCH
FIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPVESVSTLRLVSEFAEHVKKDGPFAMKDVLELLHKSLDG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]