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The map label for this gene is phrB [H]

Identifier: 222096635

GI number: 222096635

Start: 2913736

End: 2915166

Strand: Reverse

Name: phrB [H]

Synonym: BCQ_2975

Alternate gene names: 222096635

Gene position: 2915166-2913736 (Counterclockwise)

Preceding gene: 222096637

Following gene: 222096634

Centisome position: 55.91

GC content: 34.66

Gene sequence:

>1431_bases
ATGCAAAATAAAATCATCGTTATGTTTCAAAAAGATTTTCGTTTATATGATAACCCAGCTCTATTTGAAGCGGCTCAGTC
CGGTGAGGTTGTTCCGGTATATGTACATGATGAAACTTTTTCAATAGGAAGTGCGTCAAAGTGGTGGCTACACCATACTA
TAATAGATGTAAAGAGGCAACTTGAGGCATTGGGATCTACTTTAATAATCCGCAAAGGAAATACACTGGAAGAAATACTT
TCTCTCATAAAACAGTTAGGTATAACGGCTGTATATTGGAATATTTGTTATGATCCGGAAAGATTACAATTTAATCAAAA
AATGAAAATGATGTTAGAAGATAAAGGTATTACCTGTAAGGAATTTAATTCGCATTTATTATTAGAACCGTGGATTATTA
AAAAGAAAGATAACACTGAATATAAGGTTTTTACGCCTTTTTATAATGCATTTCAAAAGCAGGTAATATCTAAACCTATT
AGTAAAGTGCAGAGTATAAAATGGGGAAGTTCTTTACCAGCAAGTTTATCTGTTTCAGAATTACACTTGTTGCCGACTAT
ACCGTGGACATCTCATATGGAATCAATATGGGAGCCTACAGAAGAAGGGGCATACAAAACATTCAAGAAATTTTTCTCTA
GCAAATTAGTCTCTTATAGTGAAGGAAGGGATTTTCCAAATCAAAATGCTCATTCGATGTTGGCACCGTACCTTTCATTT
GGTCAAATATCAGTAAAGCTAATGTATCATTACTTAATAAATAAAAGTAACGAAAAACAATGTAGTCTTTTTGAACAACA
AGTGAATAGCTTTATACGTCAATTCATTTGGCGAGAGTTTTCTTATTATTTGCTATATCATTATCCATTTACAGTATATA
AACCTCTTAATAAGAACTTTGAACATTTTCCGTGGAATAATGAAGAGGAGTTATTAAGAGTGTGGCAGAAGGGCGAAACT
GGTTATCCGTTTATTGATGCAGGAATGCGGGAGTTATGGCAAACAGGTTTTATGCATAACCGAGCAAGAATGGCTGTGGC
TTCTTTTCTTGTAAAGCATTTGTTAATTCCGTGGCAAGAAGGGGCAAAATGGTTTATGGATACATTACTAGATGCTGATA
TTGCAAATAATACAATGGGGTGGCAATGGGTTGCTGGAAGTGGAGCAGATGCATCACCGTACTTTCGTATTTTTAACCCG
ATCACACAAGGTGAAAAGTTTGATAAAGATGGGGAGTATATAAGAAAATGGGTACCAGAATTAAGAGATATGCCTAATAA
ATATATACATAAACCGTGGGAAGCACCTGAGCATATTTTACAAAAAGCCAATATAAAGCTAGGAGATACATACCCTTTGC
CAGTTGTTGATCATAAGGCAGCACGAGAGAGAGCCCTTTGTGCATATAAAAGTATGAAAGAATTTATATAA

Upstream 100 bases:

>100_bases
TTGCAATTACAAAAAATGATTCTTATAATCATTTAAAAATAACAGAAGTATTTGTTCTAATAAAGAATGAATTGTGCTTT
AAGAATTAGAAAGGATAGCC

Downstream 100 bases:

>100_bases
AATACACGAGTCAATATGAATTTGATATGTAAAGGTTATAATATCTATTTACGTATTAAAGTTGTATTCATTGAAAGAGC
ATACGAATCATATAGAGTTT

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSIGSASKWWLHHTIIDVKRQLEALGSTLIIRKGNTLEEIL
SLIKQLGITAVYWNICYDPERLQFNQKMKMMLEDKGITCKEFNSHLLLEPWIIKKKDNTEYKVFTPFYNAFQKQVISKPI
SKVQSIKWGSSLPASLSVSELHLLPTIPWTSHMESIWEPTEEGAYKTFKKFFSSKLVSYSEGRDFPNQNAHSMLAPYLSF
GQISVKLMYHYLINKSNEKQCSLFEQQVNSFIRQFIWREFSYYLLYHYPFTVYKPLNKNFEHFPWNNEEELLRVWQKGET
GYPFIDAGMRELWQTGFMHNRARMAVASFLVKHLLIPWQEGAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNP
ITQGEKFDKDGEYIRKWVPELRDMPNKYIHKPWEAPEHILQKANIKLGDTYPLPVVDHKAARERALCAYKSMKEFI

Sequences:

>Translated_476_residues
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSIGSASKWWLHHTIIDVKRQLEALGSTLIIRKGNTLEEIL
SLIKQLGITAVYWNICYDPERLQFNQKMKMMLEDKGITCKEFNSHLLLEPWIIKKKDNTEYKVFTPFYNAFQKQVISKPI
SKVQSIKWGSSLPASLSVSELHLLPTIPWTSHMESIWEPTEEGAYKTFKKFFSSKLVSYSEGRDFPNQNAHSMLAPYLSF
GQISVKLMYHYLINKSNEKQCSLFEQQVNSFIRQFIWREFSYYLLYHYPFTVYKPLNKNFEHFPWNNEEELLRVWQKGET
GYPFIDAGMRELWQTGFMHNRARMAVASFLVKHLLIPWQEGAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNP
ITQGEKFDKDGEYIRKWVPELRDMPNKYIHKPWEAPEHILQKANIKLGDTYPLPVVDHKAARERALCAYKSMKEFI
>Mature_476_residues
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSIGSASKWWLHHTIIDVKRQLEALGSTLIIRKGNTLEEIL
SLIKQLGITAVYWNICYDPERLQFNQKMKMMLEDKGITCKEFNSHLLLEPWIIKKKDNTEYKVFTPFYNAFQKQVISKPI
SKVQSIKWGSSLPASLSVSELHLLPTIPWTSHMESIWEPTEEGAYKTFKKFFSSKLVSYSEGRDFPNQNAHSMLAPYLSF
GQISVKLMYHYLINKSNEKQCSLFEQQVNSFIRQFIWREFSYYLLYHYPFTVYKPLNKNFEHFPWNNEEELLRVWQKGET
GYPFIDAGMRELWQTGFMHNRARMAVASFLVKHLLIPWQEGAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNP
ITQGEKFDKDGEYIRKWVPELRDMPNKYIHKPWEAPEHILQKANIKLGDTYPLPVVDHKAARERALCAYKSMKEFI

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=479, Percent_Identity=30.2713987473904, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI4758072, Length=483, Percent_Identity=30.8488612836439, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI188536103, Length=439, Percent_Identity=29.3849658314351, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1786926, Length=480, Percent_Identity=34.5833333333333, Blast_Score=280, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6324962, Length=511, Percent_Identity=27.9843444227006, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI17137248, Length=503, Percent_Identity=28.4294234592445, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24585455, Length=503, Percent_Identity=28.4294234592445, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24648152, Length=514, Percent_Identity=24.9027237354086, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55931; Mature: 55931

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSIGSASKWWLHHTIIDVKRQ
CCCCEEEEEECCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LEALGSTLIIRKGNTLEEILSLIKQLGITAVYWNICYDPERLQFNQKMKMMLEDKGITCK
HHHCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHH
EFNSHLLLEPWIIKKKDNTEYKVFTPFYNAFQKQVISKPISKVQSIKWGSSLPASLSVSE
HCCCCEEECCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LHLLPTIPWTSHMESIWEPTEEGAYKTFKKFFSSKLVSYSEGRDFPNQNAHSMLAPYLSF
EEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
GQISVKLMYHYLINKSNEKQCSLFEQQVNSFIRQFIWREFSYYLLYHYPFTVYKPLNKNF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCCCC
EHFPWNNEEELLRVWQKGETGYPFIDAGMRELWQTGFMHNRARMAVASFLVKHLLIPWQE
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNPITQGEKFDKDGEYIRKWVPE
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCHHHCCCCCCCCHHHHHHHHHH
LRDMPNKYIHKPWEAPEHILQKANIKLGDTYPLPVVDHKAARERALCAYKSMKEFI
HHCCCHHHHCCCCCCHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSIGSASKWWLHHTIIDVKRQ
CCCCEEEEEECCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LEALGSTLIIRKGNTLEEILSLIKQLGITAVYWNICYDPERLQFNQKMKMMLEDKGITCK
HHHCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHH
EFNSHLLLEPWIIKKKDNTEYKVFTPFYNAFQKQVISKPISKVQSIKWGSSLPASLSVSE
HCCCCEEECCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LHLLPTIPWTSHMESIWEPTEEGAYKTFKKFFSSKLVSYSEGRDFPNQNAHSMLAPYLSF
EEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
GQISVKLMYHYLINKSNEKQCSLFEQQVNSFIRQFIWREFSYYLLYHYPFTVYKPLNKNF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCCCC
EHFPWNNEEELLRVWQKGETGYPFIDAGMRELWQTGFMHNRARMAVASFLVKHLLIPWQE
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNPITQGEKFDKDGEYIRKWVPE
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCHHHCCCCCCCCHHHHHHHHHH
LRDMPNKYIHKPWEAPEHILQKANIKLGDTYPLPVVDHKAARERALCAYKSMKEFI
HHCCCHHHHCCCCCCHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678007 [H]