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The map label for this gene is ysfE [H]

Identifier: 222096488

GI number: 222096488

Start: 2766252

End: 2766635

Strand: Reverse

Name: ysfE [H]

Synonym: BCQ_2828

Alternate gene names: 222096488

Gene position: 2766635-2766252 (Counterclockwise)

Preceding gene: 222096489

Following gene: 222096487

Centisome position: 53.06

GC content: 30.99

Gene sequence:

>384_bases
ATGAATTTAAAAATGAAGTACATCATTTTATATGTAGAAAAGTTTGAGGAATGTTTAAAATTCTATAAAGATATTTTACA
GTTGCCTATAAAAGCAGAGCACGGTACATATATTGAATTTAATACGGGATCTACAATATTAGCAATGAATACGCGGCAAG
ACGTGAAAGAATTAACAGGATTACCACTTACAGAAGGCGAGTTACAATCTTCTCATTTTGAATTAGGATTCGTTGTTGAA
AATGTAGAAAAAACAATTGAGCAATTTAGAGAACAAGGTATTACGATTTTAGTTGAACCAATTGTAAAGCCGTGGGGACA
AACAATTGCATATATTGCCGATCCAGATGGAAATTATATTGAAATTTGCAGTTCATTAGAATAG

Upstream 100 bases:

>100_bases
AGAAATCGGCCTCATTTTTTTATGCTCGCTACTATAGGAAACAAGGTTTTTATGAGACTTTTGTCGAAATTTTAGGGATG
GATATAAAGGGGGAAGTAAA

Downstream 100 bases:

>100_bases
AAGGAGAAAAAGAGATGGGGTTTCCGAAACTAGAAACAGAACGTTTACAATTAAGAGAATTAACACTGTTAGATGCAGAA
ACGATGTTCCGTTATTTTTC

Product: glyoxalase family protein

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNLKMKYIILYVEKFEECLKFYKDILQLPIKAEHGTYIEFNTGSTILAMNTRQDVKELTGLPLTEGELQSSHFELGFVVE
NVEKTIEQFREQGITILVEPIVKPWGQTIAYIADPDGNYIEICSSLE

Sequences:

>Translated_127_residues
MNLKMKYIILYVEKFEECLKFYKDILQLPIKAEHGTYIEFNTGSTILAMNTRQDVKELTGLPLTEGELQSSHFELGFVVE
NVEKTIEQFREQGITILVEPIVKPWGQTIAYIADPDGNYIEICSSLE
>Mature_127_residues
MNLKMKYIILYVEKFEECLKFYKDILQLPIKAEHGTYIEFNTGSTILAMNTRQDVKELTGLPLTEGELQSSHFELGFVVE
NVEKTIEQFREQGITILVEPIVKPWGQTIAYIADPDGNYIEICSSLE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14593; Mature: 14593

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKMKYIILYVEKFEECLKFYKDILQLPIKAEHGTYIEFNTGSTILAMNTRQDVKELTG
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHC
LPLTEGELQSSHFELGFVVENVEKTIEQFREQGITILVEPIVKPWGQTIAYIADPDGNYI
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEEEEECCCCCEE
EICSSLE
EHCCCCC
>Mature Secondary Structure
MNLKMKYIILYVEKFEECLKFYKDILQLPIKAEHGTYIEFNTGSTILAMNTRQDVKELTG
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHC
LPLTEGELQSSHFELGFVVENVEKTIEQFREQGITILVEPIVKPWGQTIAYIADPDGNYI
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEEEEECCCCCEE
EICSSLE
EHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]