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The map label for this gene is phoB [H]

Identifier: 222096478

GI number: 222096478

Start: 2758633

End: 2760306

Strand: Reverse

Name: phoB [H]

Synonym: BCQ_2818

Alternate gene names: 222096478

Gene position: 2760306-2758633 (Counterclockwise)

Preceding gene: 222096481

Following gene: 222096477

Centisome position: 52.94

GC content: 37.46

Gene sequence:

>1674_bases
ATGTTTGTATTGGGTTTAAAGATGAAGAAGTTACTGTTAGTAGGAACGATAACACTAAGTATGTTATGTACTGAAAGTAT
GATGGATGATCATACGGTGGAAGCGAAAGTGAAAAAAGTAGAACGACAACCGAAAAATGTAATCATGATGGTTATGGACG
GAACAAGTTCTTCAGCAACCACATTAGCTCGCTTGTATAAAGGGAAACCACTTGCATTAGATGAAATTGTAACAGGAGGA
GTTCGTACATATTCGGCGGAATCAGCTATAACAGACTCAGCTCCAGCAGCTACAGCTTTAGCAACGGGGAATAAATCGAA
TTCAGGATATGTAGGGGTATTACCTACTATTGTGAGTTCACCTGGCTTAAAACCAATAAAAGAAGAGGACAAGTTACGGC
CAGTTGCCAATGTTTTAGAAGGTGCCAAACGTACTGGTCGCGCTACTGGAATTGTTGCCACAGCTGAAATTCAGCATGCT
ACTCCTGCTGGATTCTCCGCTCATCATGTGAATCGTAAAAATTTTGAAGTGATCGCGGAGCAGCAAGTGTATCAAAATAT
AGATGTCGTATTAGGCGGGGGAAAAGCGGCACTTCTCCCTGTGAAAAGTAGTGGAATTCGAAAAGATGGTGAAGATTTAA
TAGAAGTAATTCAAAACAAAGGATACGAATTTGTGGAAACGAAAGAGGCATTATTGAAATCTAAATCTAATAAAATTTGG
GGCTCTTTTTCACATAATGCCTTAGCGTTTGATATGGACCGAGAAGCAACAAATCCAAAGCAACCAACGCTTTCGCAAAT
GACGGAAAAAGCAATTGAAACGTTATCAAAAGATAAAGACGGATTCTTTTTATTTGTGGAGGGAAGCAAGCCAGACTGGG
CAGCACATGTGAATGATCCAATTGGGATGATTAGTGATGTGTTAGCATTTGATCATGCAGTTGCAGAAGCATTAAGATTT
GCAAAAAAAGATGGTAATACGATGCTAATCGTCGTAGCGGACCATGGTAATAGCGGTATTTCTATTGGCAATATGAATAC
GACAAAAGGTTATAATACTACGCCGGTCTCTGCATATATTGATCCATTGAAAAAAGCGAAAATGACACTTGAAGGGGCTA
TCAATAATTTGAAATCTGATCTATCCAATGTAGAAGAGGTAGCTAAATTATACGGGTTGGACAATTTAACTTATGATGAA
AAAGAAAGGTTAAAAGTGGTTAAAAAGAAAATAGACGTAGGTCCAATTTTCACTACATTATTGGCTAACCGTGCGAACAT
AGGCTTTACAACAGGAGGTCATACTGGTGAGGATGTATTTTTATATTCATACGGCCCTCAAAAGCCAGTTGGTTTGATTC
AAAACACGGACGTTGCAAAGACAATAGCGAAAGCGATGGGCTTTAACTTAGAAGAAGTAACAAACAAATTATTTGTAGAG
AGTGAATTAGCTTTTAAGCAGAATGGTGCAACTGTAACAATAGATAAAACTGATGTAGCGAACCCTGTACTCATAGTAAA
ACATAATAATGTAACAGCTCAATTATTTGTTAACAAAAATATAATTCGTATTAAGAATAAAGATTATGAACTAGGAAGTG
TTGTTGTAGAAAGCAATGGTAAGTTCTATGTACCAGAAGAAGCAAGTCGTTTATTCATAAAGCATTCTCGTTAA

Upstream 100 bases:

>100_bases
TTCCTATTGTTAGCTTTAGAAAAATTGGGAACTAATAAAACATATGTAGGCAGTCAGAATTTTGTATGTAAATGAATGTG
AAACTAATGGAGGCGCAATC

Downstream 100 bases:

>100_bases
AAAAATGCCAATTCCTTAGCTACTACTTAGGAATTGGCATTTTTGTATAGATGAGATTTCACTCGAAAAAAAGAGGAAAG
AGAACCACAAATTCTAGAGT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MFVLGLKMKKLLLVGTITLSMLCTESMMDDHTVEAKVKKVERQPKNVIMMVMDGTSSSATTLARLYKGKPLALDEIVTGG
VRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSSPGLKPIKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHA
TPAGFSAHHVNRKNFEVIAEQQVYQNIDVVLGGGKAALLPVKSSGIRKDGEDLIEVIQNKGYEFVETKEALLKSKSNKIW
GSFSHNALAFDMDREATNPKQPTLSQMTEKAIETLSKDKDGFFLFVEGSKPDWAAHVNDPIGMISDVLAFDHAVAEALRF
AKKDGNTMLIVVADHGNSGISIGNMNTTKGYNTTPVSAYIDPLKKAKMTLEGAINNLKSDLSNVEEVAKLYGLDNLTYDE
KERLKVVKKKIDVGPIFTTLLANRANIGFTTGGHTGEDVFLYSYGPQKPVGLIQNTDVAKTIAKAMGFNLEEVTNKLFVE
SELAFKQNGATVTIDKTDVANPVLIVKHNNVTAQLFVNKNIIRIKNKDYELGSVVVESNGKFYVPEEASRLFIKHSR

Sequences:

>Translated_557_residues
MFVLGLKMKKLLLVGTITLSMLCTESMMDDHTVEAKVKKVERQPKNVIMMVMDGTSSSATTLARLYKGKPLALDEIVTGG
VRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSSPGLKPIKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHA
TPAGFSAHHVNRKNFEVIAEQQVYQNIDVVLGGGKAALLPVKSSGIRKDGEDLIEVIQNKGYEFVETKEALLKSKSNKIW
GSFSHNALAFDMDREATNPKQPTLSQMTEKAIETLSKDKDGFFLFVEGSKPDWAAHVNDPIGMISDVLAFDHAVAEALRF
AKKDGNTMLIVVADHGNSGISIGNMNTTKGYNTTPVSAYIDPLKKAKMTLEGAINNLKSDLSNVEEVAKLYGLDNLTYDE
KERLKVVKKKIDVGPIFTTLLANRANIGFTTGGHTGEDVFLYSYGPQKPVGLIQNTDVAKTIAKAMGFNLEEVTNKLFVE
SELAFKQNGATVTIDKTDVANPVLIVKHNNVTAQLFVNKNIIRIKNKDYELGSVVVESNGKFYVPEEASRLFIKHSR
>Mature_557_residues
MFVLGLKMKKLLLVGTITLSMLCTESMMDDHTVEAKVKKVERQPKNVIMMVMDGTSSSATTLARLYKGKPLALDEIVTGG
VRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSSPGLKPIKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHA
TPAGFSAHHVNRKNFEVIAEQQVYQNIDVVLGGGKAALLPVKSSGIRKDGEDLIEVIQNKGYEFVETKEALLKSKSNKIW
GSFSHNALAFDMDREATNPKQPTLSQMTEKAIETLSKDKDGFFLFVEGSKPDWAAHVNDPIGMISDVLAFDHAVAEALRF
AKKDGNTMLIVVADHGNSGISIGNMNTTKGYNTTPVSAYIDPLKKAKMTLEGAINNLKSDLSNVEEVAKLYGLDNLTYDE
KERLKVVKKKIDVGPIFTTLLANRANIGFTTGGHTGEDVFLYSYGPQKPVGLIQNTDVAKTIAKAMGFNLEEVTNKLFVE
SELAFKQNGATVTIDKTDVANPVLIVKHNNVTAQLFVNKNIIRIKNKDYELGSVVVESNGKFYVPEEASRLFIKHSR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=344, Percent_Identity=27.906976744186, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI157266296, Length=344, Percent_Identity=28.4883720930233, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI94721246, Length=344, Percent_Identity=28.4883720930233, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI116734717, Length=338, Percent_Identity=28.698224852071, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI294660770, Length=332, Percent_Identity=28.0120481927711, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI294660772, Length=290, Percent_Identity=28.2758620689655, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI48994877, Length=365, Percent_Identity=32.8767123287671, Blast_Score=148, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6320689, Length=525, Percent_Identity=30.0952380952381, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI85725200, Length=361, Percent_Identity=30.1939058171745, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI85815833, Length=361, Percent_Identity=30.1939058171745, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24657827, Length=475, Percent_Identity=29.4736842105263, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21355981, Length=458, Percent_Identity=28.1659388646288, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24657835, Length=467, Percent_Identity=29.1220556745182, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19921912, Length=475, Percent_Identity=27.3684210526316, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21355149, Length=339, Percent_Identity=29.7935103244838, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24657842, Length=471, Percent_Identity=27.8131634819533, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859923, Length=430, Percent_Identity=27.2093023255814, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24585213, Length=342, Percent_Identity=30.4093567251462, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355151, Length=329, Percent_Identity=28.2674772036474, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21358067, Length=480, Percent_Identity=26.875, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24651554, Length=475, Percent_Identity=25.8947368421053, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24651556, Length=453, Percent_Identity=26.0485651214128, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 60495; Mature: 60495

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVLGLKMKKLLLVGTITLSMLCTESMMDDHTVEAKVKKVERQPKNVIMMVMDGTSSSAT
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
TLARLYKGKPLALDEIVTGGVRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSS
HHHHHHCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHCC
PGLKPIKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHATPAGFSAHHVNRKNFEVIAE
CCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHCCCCCCHHHEEH
QQVYQNIDVVLGGGKAALLPVKSSGIRKDGEDLIEVIQNKGYEFVETKEALLKSKSNKIW
HHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
GSFSHNALAFDMDREATNPKQPTLSQMTEKAIETLSKDKDGFFLFVEGSKPDWAAHVNDP
ECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCH
IGMISDVLAFDHAVAEALRFAKKDGNTMLIVVADHGNSGISIGNMNTTKGYNTTPVSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHH
DPLKKAKMTLEGAINNLKSDLSNVEEVAKLYGLDNLTYDEKERLKVVKKKIDVGPIFTTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
LANRANIGFTTGGHTGEDVFLYSYGPQKPVGLIQNTDVAKTIAKAMGFNLEEVTNKLFVE
HHCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEE
SELAFKQNGATVTIDKTDVANPVLIVKHNNVTAQLFVNKNIIRIKNKDYELGSVVVESNG
HHHHEECCCCEEEEECCCCCCCEEEEEECCEEEEEEEECCEEEEECCCCEECEEEEECCC
KFYVPEEASRLFIKHSR
CEECCCCCCEEEEEECC
>Mature Secondary Structure
MFVLGLKMKKLLLVGTITLSMLCTESMMDDHTVEAKVKKVERQPKNVIMMVMDGTSSSAT
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
TLARLYKGKPLALDEIVTGGVRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSS
HHHHHHCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHCC
PGLKPIKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHATPAGFSAHHVNRKNFEVIAE
CCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHCCCCCCHHHEEH
QQVYQNIDVVLGGGKAALLPVKSSGIRKDGEDLIEVIQNKGYEFVETKEALLKSKSNKIW
HHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
GSFSHNALAFDMDREATNPKQPTLSQMTEKAIETLSKDKDGFFLFVEGSKPDWAAHVNDP
ECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCH
IGMISDVLAFDHAVAEALRFAKKDGNTMLIVVADHGNSGISIGNMNTTKGYNTTPVSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHH
DPLKKAKMTLEGAINNLKSDLSNVEEVAKLYGLDNLTYDEKERLKVVKKKIDVGPIFTTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
LANRANIGFTTGGHTGEDVFLYSYGPQKPVGLIQNTDVAKTIAKAMGFNLEEVTNKLFVE
HHCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEE
SELAFKQNGATVTIDKTDVANPVLIVKHNNVTAQLFVNKNIIRIKNKDYELGSVVVESNG
HHHHEECCCCEEEEECCCCCCCEEEEEECCEEEEEEEECCEEEEECCCCEECEEEEECCC
KFYVPEEASRLFIKHSR
CEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]