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The map label for this gene is proB [H]

Identifier: 222096470

GI number: 222096470

Start: 2746121

End: 2747224

Strand: Reverse

Name: proB [H]

Synonym: BCQ_2810

Alternate gene names: 222096470

Gene position: 2747224-2746121 (Counterclockwise)

Preceding gene: 222096472

Following gene: 222096469

Centisome position: 52.69

GC content: 40.94

Gene sequence:

>1104_bases
GTGAAGAAACAGCGAATAGTTGTAAAGATTGGGAGCAGTTCTTTGGCGGATAGTCATGGAGGCATTTCTAAAGAACAACT
TTCAGATCATGTTGCCGCATTGGCTCGATTGAAAGAGGAAGGGCATGAAGTTTTATTAATTACATCTGGGGCCGTTGCTG
CAGGTTTTTCTGCTCTAGGATACCCTAGCAGACCTGTAACAATTAAAGGAAAACAGGCTGCAGCAGCTGTCGGACAAAGT
TTATTAATGCAGGCATACACGGAGGAATTCCGTAAATATGGCATCGTTACTGCGCAACTATTGCTGACGAGAAGTGATTT
TTCTAGAAAAGAACAATATAGTAATGCTTACGCGACGTTAGGAGAGTTACTAAACCGTTCTGCTCTTCCAATTATTAATG
AAAATGATTCCATCTCTTTAGAGGAGTTAACGTTCGGTGATAATGATATGCTGTCAGCTTTAGTAAGCGGACTTGTTTCG
GCAGATATGCTGATGATTTTTACAGATGTGAACGGTTTATATGATAAAAATCCTCAAAAAAATGAGGATGCGAAAAAATA
TTATTTCCTTCCTGAAGTTACAGAAGAAATTGCTTCTCTTGCTGGAGATGCTGGCTCAAAACTTGGGACTGGCGGTATGA
AATCAAAAATTGATGCTGCAAAGACGGCGCTATCTCTTGGGGTAAGTGTATTTATCGGTACAGGACGTGGACAAGAGAAA
TTTGTTGATGTTTTAAAGGGGAAAGGTGACGGGACGTATGTCGGAAATGCCCCTCAAAAAGAAATGAAGATGAACAAGCA
ATGGATTGCCTTGCATTCGGTTGTTAGCGGTCAAATTGAAATAGACGCCGGAGCAACTACAGCTATCATTCAGCATGGCA
AGAGTCTTCTTCCTGCGGGTGTTACAAATGTGTCAGGATTTTTCAAAGTTGGCGATGTTGTAGAAGTCATGACGCAACAA
GGTCGCGTAATTGGAAAAGGACAATGCACATATAGCGCAGAGGAATTAAGAGAGATAAAAGGTATGCAAAGCCAACATAT
TCAAGCAAGAGGCGAAAGGCATAATTATGAAGTCATCCATAGGGATCATTGGGTTTCACTTTAA

Upstream 100 bases:

>100_bases
TTTGCTCTTAAATAGGCTACGTGCAGCACTTATAAGAAAGCTGTATGAATAGAAGTGTAATGTATAAAAAACTTTGATTT
TAGACTTGGGGGATAAAAAA

Downstream 100 bases:

>100_bases
GAATAGTGATGTTAAAAAGGAGGAAATAAAAATGAATGAAGTGTTAGCAAAGGGGAAAAGAGCGAAAGAGGTTGCTAGGG
AACTTGTACTAAAATCTACA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKKQRIVVKIGSSSLADSHGGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSVVSGQIEIDAGATTAIIQHGKSLLPAGVTNVSGFFKVGDVVEVMTQQ
GRVIGKGQCTYSAEELREIKGMQSQHIQARGERHNYEVIHRDHWVSL

Sequences:

>Translated_367_residues
MKKQRIVVKIGSSSLADSHGGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSVVSGQIEIDAGATTAIIQHGKSLLPAGVTNVSGFFKVGDVVEVMTQQ
GRVIGKGQCTYSAEELREIKGMQSQHIQARGERHNYEVIHRDHWVSL
>Mature_367_residues
MKKQRIVVKIGSSSLADSHGGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSVVSGQIEIDAGATTAIIQHGKSLLPAGVTNVSGFFKVGDVVEVMTQQ
GRVIGKGQCTYSAEELREIKGMQSQHIQARGERHNYEVIHRDHWVSL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=31.4685314685315, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=31.6901408450704, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=39.010989010989, Blast_Score=254, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=34.3173431734317, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=34.3173431734317, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=34.6062052505967, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321822, Length=399, Percent_Identity=33.0827067669173, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=35.5400696864112, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39554; Mature: 39554

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRIVVKIGSSSLADSHGGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCC
YPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATL
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQK
HHHHCCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
NEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
CCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHH
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSVVSGQIEIDAGATTAIIQHGKSLLPAG
HHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHCCCC
VTNVSGFFKVGDVVEVMTQQGRVIGKGQCTYSAEELREIKGMQSQHIQARGERHNYEVIH
CCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCEEEE
RDHWVSL
CCCCCCC
>Mature Secondary Structure
MKKQRIVVKIGSSSLADSHGGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCC
YPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATL
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQK
HHHHCCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
NEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
CCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHH
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSVVSGQIEIDAGATTAIIQHGKSLLPAG
HHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHCCCC
VTNVSGFFKVGDVVEVMTQQGRVIGKGQCTYSAEELREIKGMQSQHIQARGERHNYEVIH
CCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCEEEE
RDHWVSL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]