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The map label for this gene is emrB [H]

Identifier: 222096375

GI number: 222096375

Start: 2653178

End: 2654713

Strand: Reverse

Name: emrB [H]

Synonym: BCQ_2715

Alternate gene names: 222096375

Gene position: 2654713-2653178 (Counterclockwise)

Preceding gene: 222096376

Following gene: 222096373

Centisome position: 50.91

GC content: 36.46

Gene sequence:

>1536_bases
ATGGTTGAGAAGAATAATAAGCTCGGCTTTATTGTGGCGGGCTTATTGCTAGGTATTTTAATGGCATCAATGGATAATAC
CATTGTCGTAACAGCGATGGGAACGATCGTTGGTGACTTAGGAGGTCTTGAAAACTTTGTATGGGTCGTTTCTGCCTATA
TGGTCGCAGAAATGGCAGGTATGCCGATTTTCGGTAAACTATCAGATATGTATGGAAGAAAGAGATTCTTTATTTTTGGT
TTAATCGTCTTTATGATTGGTTCGGCACTTTGTGGTACTGCTGAAAACATTACACAGTTAGGCATTTATCGTGCCATTCA
AGGTATTGGCGGCGGGGCATTAGTACCGATTGCATTTACTATTGTTTTTGATATTTTCCCTCCTGAAAAGCGCGGGAAAA
TGGGTGGTTTATTCGGAGCAGTATTTGGATTATCAAGTATTTTCGGGCCATTACTTGGCGCATATATTACAGATTATATT
AGTTGGCACTGGGTATTTTACATTAACTTACCTCTTGGCATTTTAGCACTTATTTTTATTACATTCTTTTATAAAGAATC
ACGAGTTCATAGAAAACAAAAAATTGATTGGTTCGGCGCAATTACTTTAGTTGGTGCAGTAGTTTCTTTAATGTTTGCTT
TAGAACTTGGTGGACAAAAGTATGACTGGGATTCTAGCTTTATTTTAAGTTTATTTGGTGGATTCGCTATTTTAATTATT
GCATTTATTTTTATTGAACGAAAAGTAGAAGAGCCAATCATATCATTTGAGATGTTTAAGCAGCGCTTATTCGGAATGAG
TACCATTATTGCTTTATGTTATGGTGCTGCGTTTATGTCAGCAACTGTATACATTCCGTTATTCATTCAAGGTGTATACG
GAGGTACCGCAACAAACTCAGGGTTATTACTATTACCGATGATGTTAGGATCAGTTGTAACAGCGCAATTAGGTGGGTTT
TTAACGACGAAGCTTAGCTACCGAAACATTATGATTATTTCTGCTGTTATTATGCTAATTGGACTATTTTTATTAAGCAC
ATTAACGCCAGAAACAAGTCGTGTATTATTAACAGTGTATATGATTATTATTGGATTTGGAGTTGGTTTCTCATTCTCTG
TACTAAGTATGGCGGCTATCCACAACTTTGGTATGGAACAGCGAGGGTCTGCGACTTCAACGAGTAACTTCATTCGTTCA
TTAGGCATGACGCTTGGTATTACAATCTTCGGAATGATCCAAAGAACAGGTTTCCAAGATCAATTAGAAGAAGCATTTAA
AGGTATGAACGGGGGAATGAATACAAACGCTTTAGGAGATTCAAGAGCTATTTTATCAGAATCGGCAAGATCTCAAATTC
CACCGCAAATATTAGATAAAATTATTGATGCTCTTTCTAGCTCAATTGTCCAAACATTTATGTGGGCATTGGTACCAGCT
GGTTTAGCATTCATATTTATTTTCTTTATGGGAAATGAGCGCATGGTAATTAAAAAACAACAAAAGAATAAAAAGAGCGA
AACATCAAAAGCGTAA

Upstream 100 bases:

>100_bases
AAATTAGTGTTGTAGAATATCATACTAGTTTTAATCAAAAGGGTAATACAGTTGTAAAAGAACATAAACAAATATAAGTA
GTATGAGGGAGAGCAAGGTT

Downstream 100 bases:

>100_bases
TGAAAAAGCTCCCTTCCAATTGGAAAGGAGCTTTTTCGTATGTACGGATTTAGATTAGCCTTCGATGATTTCGTATTCTT
CGTCTGCTAATAATAAAATT

Product: multidrug resistance protein b

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVEKNNKLGFIVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRVLLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMNGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVIKKQQKNKKSETSKA

Sequences:

>Translated_511_residues
MVEKNNKLGFIVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRVLLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMNGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVIKKQQKNKKSETSKA
>Mature_511_residues
MVEKNNKLGFIVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRVLLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMNGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVIKKQQKNKKSETSKA

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=434, Percent_Identity=29.7235023041475, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87081983, Length=404, Percent_Identity=28.960396039604, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788710, Length=438, Percent_Identity=27.6255707762557, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=25.1781472684086, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=25.0620347394541, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI48994889, Length=134, Percent_Identity=30.5970149253731, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=554, Percent_Identity=23.6462093862816, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6319770, Length=466, Percent_Identity=25.5364806866953, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319783, Length=457, Percent_Identity=23.8512035010941, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323735, Length=327, Percent_Identity=30.2752293577982, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=529, Percent_Identity=23.062381852552, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321663, Length=540, Percent_Identity=22.4074074074074, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324954, Length=401, Percent_Identity=24.1895261845387, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323520, Length=410, Percent_Identity=24.6341463414634, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55810; Mature: 55810

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKNNKLGFIVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGILALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRVLLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEAFKGMNGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFIFIFFMGNERMVIKKQQKNKKSETSKA
HHHHHHHHHCCCCEEEEEEHHHCCCCCCCCC
>Mature Secondary Structure
MVEKNNKLGFIVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGILALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRVLLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEAFKGMNGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFIFIFFMGNERMVIKKQQKNKKSETSKA
HHHHHHHHHCCCCEEEEEEHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]