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The map label for this gene is narH [H]

Identifier: 222095763

GI number: 222095763

Start: 2075189

End: 2076550

Strand: Direct

Name: narH [H]

Synonym: BCQ_2103

Alternate gene names: 222095763

Gene position: 2075189-2076550 (Clockwise)

Preceding gene: 222095762

Following gene: 222095764

Centisome position: 39.8

GC content: 38.84

Gene sequence:

>1362_bases
ATGTATTTCAATAACGTAGAAACGAAACCTGGCATTGGTTATCCGAAGCAATGGGAAGATCAGGAGAAATATAAAGGTGG
CTGGGAATTGAAAAATGGTGAAATTCAGCTGAAATCCGGTTCGAAAATGAAGCGCCTTATGAATATTTTCCACAATCCAG
ATCAACCAACAATTGATGATTACTTTGAACCGTGGAACTATGATTATGAAACGTTAACAAATAGTCCGCAGCGTAAGCAT
CAGCCAGTTGCTCGTCCGAAATCAGCAATTACAGGCGAATTTATCGACAAAATTGAATGGGGTCCAAACTGGGAAGATGA
TTTAGCTGGTGGACATATAACAGGATTACAAGATCCGAACGTAAAGAAAATGGAAGAAGAAATTAAAACCGATTTTGAAA
ATGTCTTTATGATGTATTTACCACGCATATGCGAACATTGTATGAATCCATCTTGCGTATCGTCTTGTCCATCTGGTGCG
ATGTATAAACGGGAAGAAGATGGGATTGTTCTTGTGGATCAAAACGCATGTCGTGCATGGAGATTCTGCGTATCGTCTTG
CCCATATAAAAAAGTGTACTTTAATTGGCAAACGAATAAAGCGGAAAAATGTACAATGTGTTTCCCACGTATTGAAGCTG
GTATGCCAACAATTTGTTCGGAAACATGTGTAGGACGTATCAGATATATTGGGGTAATGCTATATGACGCTGACAAAGTA
AAAGAAGCGGCTTCTGTTGAGAATGAAAAAGATTTATATGAATCTCAGCTTACTGTTTTCCTAGATCCAAATGATCCTGA
AGTAGCAGCTGAAGCGAAAAAACAAGGCATTCCTGAAGAGTGGATTAAAGTGGCACAACAGTCACCAATCTATAAAATGA
TTATCGATTGGAAAATTGCTCTACCACTTCACCCAGAGTATCGTACAATGCCAATGGTTTGGTATATCCCACCGCTTAGC
CCAATTATGAATATGGTGGAAGGGAAAGGTAGTAACTGGCAAGCAGAAGAGATTTTCCCTGCTATCGATAATATGCGTAT
TCCAATTCAATATTTAGCGAATCTACTCACTGCAGGAGATGAATCACACATTCGACTTACATTGAAAAAAATGGCTGTTA
TGCGAACGCATATGAGAGCACTGCAAATTAATAAAGAACCAAATGAAGCTGTATTAAAAGAACTTGGTTTAACGAAACAG
GATGTAGAAGATATGTATCGTCTTCTTGCCATTGCAAAATATAAAGATCGCTTCGTCATCCCGACATCTCACCGTGAACA
AGTGGCAGATTTATATAGCGAACAAGGAAGCTGTGGTTTATCATTCACAGGTGGTCCAGGCTCTTGTATGACAATTTCTT
AA

Upstream 100 bases:

>100_bases
ACAAGTCGGAATGGTAATGAACCTAGATAAATGCATCGGCTGCCATACTTGTAGCGTAACGTGCAAAAACACCTGGACAA
ATCGTCCAGGTGCTGAATAT

Downstream 100 bases:

>100_bases
ATAAAGGGGCAGAAAATTATGAGACAAAGTTTACAAACTGCTTTTTCTTGTTCTTCATTCCTTCTCTCTTACCCGGAACT
TGGGTGGAGAGAAGCTTTAA

Product: respiratory nitrate reductase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKVAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSFTGGPGSCMTIS

Sequences:

>Translated_453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKVAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSFTGGPGSCMTIS
>Mature_453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKVAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSFTGGPGSCMTIS

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=445, Percent_Identity=58.4269662921348, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1787478, Length=448, Percent_Identity=57.5892857142857, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=35.6060606060606, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=35.6060606060606, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 52044; Mature: 52044

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDD
CCCCCCCCCCCCCCCCCCCCHHHHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHH
YFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPN
HCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCC
VKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHH
RFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
HHHHHCCCCEEEEEEECCCCCCCHHEEHHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHH
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKVAQQSPIYKMIIDWKIA
HHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEE
LPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGD
ECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCC
ESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVI
CCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEE
PTSHREQVADLYSEQGSCGLSFTGGPGSCMTIS
CCCHHHHHHHHHHCCCCCCEEECCCCCCEEEEC
>Mature Secondary Structure
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDD
CCCCCCCCCCCCCCCCCCCCHHHHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHH
YFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPN
HCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCC
VKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHH
RFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
HHHHHCCCCEEEEEEECCCCCCCHHEEHHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHH
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKVAQQSPIYKMIIDWKIA
HHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEE
LPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGD
ECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCC
ESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVI
CCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEE
PTSHREQVADLYSEQGSCGLSFTGGPGSCMTIS
CCCHHHHHHHHHHCCCCCCEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]