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The map label for this gene is gatA [H]

Identifier: 222095714

GI number: 222095714

Start: 2031577

End: 2033052

Strand: Direct

Name: gatA [H]

Synonym: BCQ_2054

Alternate gene names: 222095714

Gene position: 2031577-2033052 (Clockwise)

Preceding gene: 222095713

Following gene: 222095716

Centisome position: 38.96

GC content: 36.59

Gene sequence:

>1476_bases
ATGGAGATTCAGTTCAATACACTACTACAAAAAGAATTAACAATTCATGATATACAAAGAGCGATGGAAGATGGAAAGTT
AACTTCAAAAGAACTAGTTATGTATTACCTTCATAGAATCGCAAAGTATGATCAAGATGGACCGGAAATTAATTCTATTT
TAGAAATCAATCCAGACGCCATCTTTATTGCTGAAGCGTTAGATTATGAAAGAAAGATTAAAGGTAAAAGAGGTCCATTA
CATGGTATACCGGTTTTACTTAAGGATAATATTGAAACGAATGACTCTATGCATACAAGTGCAGGTACAATTGCGCTAGA
ACAACATATAAGTAGTGAAGATGCATTTCTCGTTACGAAGCTGCGAGAAGCAGGAGCAGTGATAATAGGGAAAACAAATA
TGACAGAATTAGCAAATGCAATGTCATTTGAAATGTGGGCTGGATATAGTGCAAGAGGTGGGCAAACAATAAACCCTTAC
GGTACGGGTGAGGATGATATGTTTGTTGGTGGCTCAAGTACAGGATCTGCCATAGCAGTTGCTGCTAACTTTACAGTAGT
ATCGGTTGGAACAGAAACAGATGGTTCTATTTTGAGTCCAGCTGTTCAAAACTCTGTAGTCGGTATTAAACCGACTGTTG
GTTTAATTAGTCGTAGAGGTATTATTCCGTTCACATATTCGCAAGATACAGCCGGCCCATTTGCTAGAACAGTAACAGAT
GCTGCTATTTTACTAGGGGGGTTAACTGGGGTGGATGAGAGAGATGTAGCTACTCGTAAAAGTGAAGGGATAGCAGAACA
TGATTATACAAAGTATTTGGATGTTAACGGTTTAAATGGAACAAAGATTGGTGTATATAACAACGCACCAAAAGATTATT
ATAAATCTGGTGAGTATGATGAGAATTTGTTTAAGGAAACCATTGAAGTATTACGTAGTAAGGGTGCGACAGTAGTAGAG
GATATTAATATTCCGTCTTTTCATAGAGAATGGAGCTGGGGTGTATCGCTTTATGAATTAAAGCATAGTTTAGATAACTA
TCTTTCGAAATTGCCTTCTACTATACCAGTCCATTCTATTTCAGAGTTAATGGAGTTTAATGAAAACATAGCAGAAAGAG
CTCTGAGATATGGACAAACTAAGTTAGAAAGAAGAAAAGATTTTCCTAATACGTTAAGAAATCCACAATATTTAAATGCA
AGATTAGAAGATATATATTTTTCACAAGAACAAGGCATTGATTTTGCACTGGAAAAATATAATCTTGATGCGATTCTATT
CCCTTCGTATATAGGTTCCACTATATGTGCGAAAGCAGGTTATCCGTCTATAGCAATACCTGCGGGATATATGGATAACG
GGAGACCATTTGGAATTACGCTTGCAAGCACTGCTTTTCATGAGGGGACTTTAATTAAACTCGCATACGCTTTTGAACAA
GCGACAAAGCATCGGAAATCTCCTAACTTATCATAA

Upstream 100 bases:

>100_bases
ATTCTTATAGAGGGATTGTAGTTGTATATGTGAGGAGCGGAGAAAATGAAAAAGTAATTGAGATATGCTATTAATAAAGG
TTTATAGAAGGAGAATAAAA

Downstream 100 bases:

>100_bases
ATAAATAGGGCAGGAAATCCTGTCCTATTTATTTTGTTCATTAAAGTGACATGTTTTTTTGCTTTGTCTTATCATATTTT
AATAAGTTAAATGATAAACA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEIQFNTLLQKELTIHDIQRAMEDGKLTSKELVMYYLHRIAKYDQDGPEINSILEINPDAIFIAEALDYERKIKGKRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVIIGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGEDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGGLTGVDERDVATRKSEGIAEHDYTKYLDVNGLNGTKIGVYNNAPKDYYKSGEYDENLFKETIEVLRSKGATVVE
DINIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALRYGQTKLERRKDFPNTLRNPQYLNA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMDNGRPFGITLASTAFHEGTLIKLAYAFEQ
ATKHRKSPNLS

Sequences:

>Translated_491_residues
MEIQFNTLLQKELTIHDIQRAMEDGKLTSKELVMYYLHRIAKYDQDGPEINSILEINPDAIFIAEALDYERKIKGKRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVIIGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGEDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGGLTGVDERDVATRKSEGIAEHDYTKYLDVNGLNGTKIGVYNNAPKDYYKSGEYDENLFKETIEVLRSKGATVVE
DINIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALRYGQTKLERRKDFPNTLRNPQYLNA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMDNGRPFGITLASTAFHEGTLIKLAYAFEQ
ATKHRKSPNLS
>Mature_491_residues
MEIQFNTLLQKELTIHDIQRAMEDGKLTSKELVMYYLHRIAKYDQDGPEINSILEINPDAIFIAEALDYERKIKGKRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVIIGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGEDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGGLTGVDERDVATRKSEGIAEHDYTKYLDVNGLNGTKIGVYNNAPKDYYKSGEYDENLFKETIEVLRSKGATVVE
DINIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALRYGQTKLERRKDFPNTLRNPQYLNA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMDNGRPFGITLASTAFHEGTLIKLAYAFEQ
ATKHRKSPNLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=536, Percent_Identity=26.4925373134328, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI195972892, Length=251, Percent_Identity=28.6852589641434, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=486, Percent_Identity=24.8971193415638, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=23.3870967741935, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=414, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=207, Percent_Identity=36.7149758454106, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648113, Length=508, Percent_Identity=25.5905511811024, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356731, Length=252, Percent_Identity=30.1587301587302, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648435, Length=256, Percent_Identity=28.125, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648437, Length=256, Percent_Identity=28.125, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550774, Length=256, Percent_Identity=28.125, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644968, Length=512, Percent_Identity=22.265625, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24652985, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19922090, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24652981, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24652983, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=30.6818181818182, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=30.6818181818182, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 54214; Mature: 54214

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIQFNTLLQKELTIHDIQRAMEDGKLTSKELVMYYLHRIAKYDQDGPEINSILEINPDA
CEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDYERKIKGKRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTK
EEEEHHHCHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEHEE
LREAGAVIIGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGEDDMFVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEE
AANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
EEEEEEEEECCCCCCCHHCHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGGLTGVDERDVATRKSEGIAEHDYTKYLDVNGLNGTKIGVYNNAPKDYYKSGEYD
HHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHCCCCCC
ENLFKETIEVLRSKGATVVEDINIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSI
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALRYGQTKLERRKDFPNTLRNPQYLNARLEDIYFSQEQGIDFALEKY
HHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHCCCHHHHHHHHHHEECHHCCCEEEEEHC
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMDNGRPFGITLASTAFHEGTLIKLAYAFEQ
CCCEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHH
ATKHRKSPNLS
HHHHCCCCCCC
>Mature Secondary Structure
MEIQFNTLLQKELTIHDIQRAMEDGKLTSKELVMYYLHRIAKYDQDGPEINSILEINPDA
CEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDYERKIKGKRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTK
EEEEHHHCHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEHEE
LREAGAVIIGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGEDDMFVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEE
AANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
EEEEEEEEECCCCCCCHHCHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGGLTGVDERDVATRKSEGIAEHDYTKYLDVNGLNGTKIGVYNNAPKDYYKSGEYD
HHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHCCCCCC
ENLFKETIEVLRSKGATVVEDINIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSI
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALRYGQTKLERRKDFPNTLRNPQYLNARLEDIYFSQEQGIDFALEKY
HHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHCCCHHHHHHHHHHEECHHCCCEEEEEHC
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMDNGRPFGITLASTAFHEGTLIKLAYAFEQ
CCCEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHH
ATKHRKSPNLS
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA