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The map label for this gene is brnQ [H]

Identifier: 222095707

GI number: 222095707

Start: 2025339

End: 2026685

Strand: Direct

Name: brnQ [H]

Synonym: BCQ_2047

Alternate gene names: 222095707

Gene position: 2025339-2026685 (Clockwise)

Preceding gene: 222095706

Following gene: 222095708

Centisome position: 38.84

GC content: 35.34

Gene sequence:

>1347_bases
ATGAAAAGCATGAAGGGACGTTTAAGGCCAGGTGATACGGTAGCAATTGGTTTAATGTTATTTGCACTATTTTTAGGAGC
AGGAAATTTAATTTTTCCACCAGTTTTAGGGCAACAAGCTGGAGAGAATGTTTGGGTTGCTACAATTGGATTCCTTGTAA
CGGGAGTCGGATTACCCCTATTAGCTGTAACAGCTGTCGCGTTTGTAGAGGGGGACTTGAAAGCGCTATCTTCTAGAGTT
CACCCTATATTTGCGTTTGTTTTCCCATTAATTAGTTATTTAGCGATTGGACCTTTTTTCGCGATTCCGCGTACGGGAGC
CGTTTCATTTGAAATGGGTATGAAGCCATTTTTATCAGAGGCATTAGTTTCCGAATGGTACATGCTATTTCTTTTCACTA
TAATTTTCTTCGGAATAACTTGGTATTTATCTTTAAATCCATCAAAATTAGTGGATTGGTTCGGGAAATTTCTTACACCA
TTGTTAGTATTAATTGTTGCTGTAATTGTCGGAAAGGCAATTGTTGATCCGATTGGAGAACCAGCTGCACCGCTAGAGGC
ATACAAGGAAAATGCTTTCTTTGGTGGATTTATTCAAGGATATTTAACGATGGATGCAATTAGTGCGCTTGTATTTGGAA
TTGTAGTTGTACAAGTTATCCGCTCTAAAGGAATAAAAGAGAGTAATCAAATTGCAAAAATAACAGTAGTATCAGGTATT
ATCGCTGTACTTGGTTTAACGTTAATCTATTTATCACTTGCTTATCTTGGTTCAACAAGTACATCACTTGGGGTATCAGA
GAATGGTGGACTTATTTTAACGAATGTTGTAAATGAGTTATATGGAACAAGTGGTAAAATTTTATTAGGACTTGTTATTG
TACTCGCTTGTTTAACAACTTCTGTTGGATTAACATCAGCCTGTGCTGGCTTCTTTACAAACTTATTCCCAAAACTTTCG
CATAAAACGATTGTAACAATGGTATGTGTATTTAGTTTAATTGTATCTAACCTAGGTTTAACACAATTAATCGCAGTTAC
TTTACCAGTGTTAATGATTATTTATCCAGTTGCAATTGTATTAATTGTACTTTCTTACTTCCATAAATGGATTGGGAAGC
GTAATACCATTTATATTGGAGCTATTTTAGGTGCGTTGTTAATTAGTTTCTTTAACGGTTTAGAAAGTGCGAATATTAAA
ATTGACGCGATTTCTAATGTACTACAAATGTTACCATTATATAAAGAAGGAATAGGATGGTTAATCCCATCATGTATTGG
CGGGATCCTCGGTTTCTTCTTCTATAAATCAAATGAATCAAGTGAATTACAAAAGAAAGGTGCTTAG

Upstream 100 bases:

>100_bases
ACACTGCTTTGTAAGGGGCATATTTTATCAGCTTGAACAGAGAGGAGGATGCATAGCATATTGTGAAAATAGAGATAAAC
AATGAAAAAGTGCTATGCAA

Downstream 100 bases:

>100_bases
GCGCCTTTCTTTTTTTAAAGGGGATTTTTTTGTTTTGTCTAAGTAATAAATGAAAGGAGTGATAAAATGGCTAATTTTGA
AGATTTTTTAACTTTGGATT

Product: branched-chain amino acid transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKSMKGRLRPGDTVAIGLMLFALFLGAGNLIFPPVLGQQAGENVWVATIGFLVTGVGLPLLAVTAVAFVEGDLKALSSRV
HPIFAFVFPLISYLAIGPFFAIPRTGAVSFEMGMKPFLSEALVSEWYMLFLFTIIFFGITWYLSLNPSKLVDWFGKFLTP
LLVLIVAVIVGKAIVDPIGEPAAPLEAYKENAFFGGFIQGYLTMDAISALVFGIVVVQVIRSKGIKESNQIAKITVVSGI
IAVLGLTLIYLSLAYLGSTSTSLGVSENGGLILTNVVNELYGTSGKILLGLVIVLACLTTSVGLTSACAGFFTNLFPKLS
HKTIVTMVCVFSLIVSNLGLTQLIAVTLPVLMIIYPVAIVLIVLSYFHKWIGKRNTIYIGAILGALLISFFNGLESANIK
IDAISNVLQMLPLYKEGIGWLIPSCIGGILGFFFYKSNESSELQKKGA

Sequences:

>Translated_448_residues
MKSMKGRLRPGDTVAIGLMLFALFLGAGNLIFPPVLGQQAGENVWVATIGFLVTGVGLPLLAVTAVAFVEGDLKALSSRV
HPIFAFVFPLISYLAIGPFFAIPRTGAVSFEMGMKPFLSEALVSEWYMLFLFTIIFFGITWYLSLNPSKLVDWFGKFLTP
LLVLIVAVIVGKAIVDPIGEPAAPLEAYKENAFFGGFIQGYLTMDAISALVFGIVVVQVIRSKGIKESNQIAKITVVSGI
IAVLGLTLIYLSLAYLGSTSTSLGVSENGGLILTNVVNELYGTSGKILLGLVIVLACLTTSVGLTSACAGFFTNLFPKLS
HKTIVTMVCVFSLIVSNLGLTQLIAVTLPVLMIIYPVAIVLIVLSYFHKWIGKRNTIYIGAILGALLISFFNGLESANIK
IDAISNVLQMLPLYKEGIGWLIPSCIGGILGFFFYKSNESSELQKKGA
>Mature_448_residues
MKSMKGRLRPGDTVAIGLMLFALFLGAGNLIFPPVLGQQAGENVWVATIGFLVTGVGLPLLAVTAVAFVEGDLKALSSRV
HPIFAFVFPLISYLAIGPFFAIPRTGAVSFEMGMKPFLSEALVSEWYMLFLFTIIFFGITWYLSLNPSKLVDWFGKFLTP
LLVLIVAVIVGKAIVDPIGEPAAPLEAYKENAFFGGFIQGYLTMDAISALVFGIVVVQVIRSKGIKESNQIAKITVVSGI
IAVLGLTLIYLSLAYLGSTSTSLGVSENGGLILTNVVNELYGTSGKILLGLVIVLACLTTSVGLTSACAGFFTNLFPKLS
HKTIVTMVCVFSLIVSNLGLTQLIAVTLPVLMIIYPVAIVLIVLSYFHKWIGKRNTIYIGAILGALLISFFNGLESANIK
IDAISNVLQMLPLYKEGIGWLIPSCIGGILGFFFYKSNESSELQKKGA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=435, Percent_Identity=42.5287356321839, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48032; Mature: 48032

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]