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The map label for this gene is cls [H]

Identifier: 222095699

GI number: 222095699

Start: 2018390

End: 2019523

Strand: Direct

Name: cls [H]

Synonym: BCQ_2039

Alternate gene names: 222095699

Gene position: 2018390-2019523 (Clockwise)

Preceding gene: 222095698

Following gene: 222095700

Centisome position: 38.71

GC content: 34.66

Gene sequence:

>1134_bases
ATGAACATCGATGTGGAAATGGGGAAAAAAATGGCTAGTGAATATGAAGTAGGACAAGTTAGATTAGGTGAATTTCAACT
GTATACGAACGGTGAAGAGTTATATACGAATTTATTTGAGGATATACGTAATGCGGAGAAGTACATATATATCCATTTTT
ATATTGTAGGTAAAGATGAAATAAGCAAAGAGTTTTTGCAGTTGCTCGAGAAAAAGGCATCTAGCGGAGTAGAAGTGAAA
TTGTCAGTCGATCGAATAGGTGGATATAAGCTGAAGAAAAAGGTAATTCGTCAACTACAAAGCAATGGTGTCCAGTTTAC
GTTTAGTAAAAAACCTAAATTGAAACATTTATTCTATTCGTTGCATCAGCGAAATCATAGACGAATTGTTACGATAGACG
GAAAAGTATCATATATCGGCGGCTTTAACATCGGAAAGGAATACCTTGGACAAGATCCGAAATTTGGCCAGTGGCGTGAT
TATCATGTGCGAGTTAAGGGGAATGGTGCCACGGATATGGAAAGAAAATTTGCTGCTGACTGGAAAGAAGATACGGGAAA
AAAAATGCCTGTACATGAAAGTCTACCTGCAATTGGGAATGTAAAATATCAATATTTATTTTCAGATGGAAAAGGGTTAT
GGGAAAAATATGGAGCGTTTTTAAAGCAGGCTAAAAAGTCTTTAATTATTGCTACACCATATTTTGTACCAAGTAAAGAA
ATGATGAAAGAGTTAAAAGGTGCATTAAACCGCGGTGTCAATGTGAAAATTCTCGTACCATTTAAAAGTGATGCAGTATT
GTTAAAACAAGCTGCCTATCCGTATTTGAGAGAGATGAACCATGCTGGAGCGGAGATCTATCAATATCGGAATGGTTTTT
TTCACGGGAAAGTAACAATCATTGATGGAGAAATTGTTGATATAGGTACAGCGAATTTTGATAATAGAAGTTTTTATTTA
AATTGTGAATCTAATTGTGTCGTATATGATAAAACAGTTGTTGCTGACGTATGGGATCGACTAAAAGAAGACTTCCATAA
ATCAAAAAGATTTTCGGAAGAAGATTTTGAGAAAATTAGTAAATGGGATTGGTTTTTAGCAAGAATAGCAAATGTTATAG
CATCATATTTATAG

Upstream 100 bases:

>100_bases
AAAAAGGGAAGATATAGAAATAACAGTAAGTGGAGAAAGGAAAATATCCATGAAACACTTTTTCGTTGTAATCCTTTGTG
TAATAGGTGTATTCATTTGG

Downstream 100 bases:

>100_bases
CATATAGACAAAAAGTAGGGAGGCACTTTCATGGATTGTATGAAAGAATTGATGAGGTATAGTTATATGCTTATATATAA
GGTTGGGGAGTATACAGGAC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNIDVEMGKKMASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASSGVEVK
LSVDRIGGYKLKKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGQWRD
YHVRVKGNGATDMERKFAADWKEDTGKKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKE
MMKELKGALNRGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGTANFDNRSFYL
NCESNCVVYDKTVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Sequences:

>Translated_377_residues
MNIDVEMGKKMASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASSGVEVK
LSVDRIGGYKLKKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGQWRD
YHVRVKGNGATDMERKFAADWKEDTGKKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKE
MMKELKGALNRGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGTANFDNRSFYL
NCESNCVVYDKTVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL
>Mature_377_residues
MNIDVEMGKKMASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASSGVEVK
LSVDRIGGYKLKKKVIRQLQSNGVQFTFSKKPKLKHLFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGQWRD
YHVRVKGNGATDMERKFAADWKEDTGKKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKE
MMKELKGALNRGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTIIDGEIVDIGTANFDNRSFYL
NCESNCVVYDKTVVADVWDRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=350, Percent_Identity=29.4285714285714, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1787007, Length=323, Percent_Identity=26.9349845201238, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 43693; Mature: 43693

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDVEMGKKMASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDE
CCCCHHHHHHHHHCCCCCEEEECEEEEEECHHHHHHHHHHHHCCCCEEEEEEEEEECCHH
ISKEFLQLLEKKASSGVEVKLSVDRIGGYKLKKKVIRQLQSNGVQFTFSKKPKLKHLFYS
HHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
LHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGQWRDYHVRVKGNGATDMERKFAAD
HHCCCCCEEEEECCEEEEEECCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCC
WKEDTGKKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKE
CHHCCCCCCCCHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCHH
MMKELKGALNRGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTI
HHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHCCEEEEEEEE
IDGEIVDIGTANFDNRSFYLNCESNCVVYDKTVVADVWDRLKEDFHKSKRFSEEDFEKIS
EECEEEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KWDWFLARIANVIASYL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIDVEMGKKMASEYEVGQVRLGEFQLYTNGEELYTNLFEDIRNAEKYIYIHFYIVGKDE
CCCCHHHHHHHHHCCCCCEEEECEEEEEECHHHHHHHHHHHHCCCCEEEEEEEEEECCHH
ISKEFLQLLEKKASSGVEVKLSVDRIGGYKLKKKVIRQLQSNGVQFTFSKKPKLKHLFYS
HHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
LHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQDPKFGQWRDYHVRVKGNGATDMERKFAAD
HHCCCCCEEEEECCEEEEEECCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCC
WKEDTGKKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGAFLKQAKKSLIIATPYFVPSKE
CHHCCCCCCCCHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCHH
MMKELKGALNRGVNVKILVPFKSDAVLLKQAAYPYLREMNHAGAEIYQYRNGFFHGKVTI
HHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHCCEEEEEEEE
IDGEIVDIGTANFDNRSFYLNCESNCVVYDKTVVADVWDRLKEDFHKSKRFSEEDFEKIS
EECEEEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KWDWFLARIANVIASYL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]