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The map label for this gene is oppA [H]

Identifier: 222095686

GI number: 222095686

Start: 2005977

End: 2007638

Strand: Direct

Name: oppA [H]

Synonym: BCQ_2026

Alternate gene names: 222095686

Gene position: 2005977-2007638 (Clockwise)

Preceding gene: 222095685

Following gene: 222095687

Centisome position: 38.47

GC content: 33.75

Gene sequence:

>1662_bases
ATGAAGAGAAAGGTAACAACGATTGCTGCAGTTGCATTATCGACTAGTGTATTAGTTGCAGGTTGCGGAAATGGGGAGAA
GGCATCTACAACGAAGAAAGAAGCAGGTAAGGGAGCAGCGGATAAGCAAGTATTAAACTTACTAGAAACAGCAGAAATTC
CTTCAATGGATACGTCAAAATCAACAGACTCTGTATCATTCCGTGCCTTTGTAAATGCAATGGAAGGATTATATCGTTTA
GATAAGGATAATAAACCAACTCCAGGTATGGCAAAAGACGTGAAAATTAGTGAAGATAAAAAGACATATACATTTGAATT
ACGTGATGCAAAATGGTCAAATGGTGATCCAGTTCGAGCGCAAGATTTTGTATATGCATGGCAACGTACATTAGATAAAG
CAACGGCAGCTGAGTATGCATTTATTTTATTTGATGTGAAAAATGCACAGAAAGTAAATAAAGGAGAGTTACCGTTAGAT
CAATTAGGAGTGAAAGCGAAGGATGATAAAACGTTAGTAGTAGAATTAGAACAACCAGCACCATATTTTCTTGAGCTTAC
TTCATTCCCAACATTCTTCCCTTTAAATGAAAAATATGTGAAAGAACAAGGAGATAAATACGGATTAGAAGCAGATAAAT
TATTATATAATGGACCGTTTACGATGAGTGAATGGAAACATGAAAGAAGTTATCAGTTAAAGAAAAATCCTAATTATTGG
GATAAAGATACTGTGAAGTTAGAAGAAATTAATGTGAATATTGTGAAGGAAACAAGCACAGGAGTAAATTTATATGATAG
TGATCAAGCGGACCGTGTTATTTTAACTTCTGAATTTGTTGATAAATATAAAAAGAATAAGCCAGATGAATTTAAAACGA
AGTTAGATCCGCGTATGTATTTCTTACGATTTAACCAAAAAAATGCAACGTTTAAAAACCAAAAGGTTCGTGAAGCAATT
GATCTAGCATATGATAAAAAAGGAATTGCAAATGTTATTTTAAATGACGGATCATTGCCAGCATATTTCTTAGTACCTGA
AAAATTTACGACAGGACCTGATGGCAAAGATTTCCGTACTGTAAATAAAAATTTCCGTGACAGTAAAGATAATGCAGCAA
AAGCGAAAAAATTATGGGAAGAAGCAAAGAAAGAACTTGGCCAAGATACAATTACAGTCGAATTACTAAATGATGATAAC
GGTAGTCGTAAAAAAGTAGGAGACTTTATTAAAGAAGAGTTAGAGAAAAATTTACCTGGTATTAAAGTGAATTTAAAACA
ACAGCCATATAAGCAGAAGTTGGATTTAGAAAAGAAATTTGAATATGAATTTTCGTTATCTGCATGGAGTCCAGATTATC
CAGATCCAATGACTTATATTGATATGTTTGTTACTGGAAGCTCATTTAATGAAATGGACTATTCTAATCCGAAGTATGAT
GAGTTAGTAGCGAAATCAAAAGGAGAACTATTAAAAGATGATAAAGCACGCTGGAATGCACTTGCAGAAGCGGAAAAAAT
CTTAATTGGTCAAGATGCTGCCATTTCTCCTACGTACCAACAAAGTACCGCATACTTAGAGAAACCATACGTAAAAGGTA
TTGCAAATCATACGTTTGGTGGGGACTTCAGTTATAAATGGGCATATATTACAAAAAAATAG

Upstream 100 bases:

>100_bases
TGTATATACGAAAATAATATTTGAAATAATCTGAATCTTTTGTATAATAATATGTATTATACTGGCTTTCATTTATACAT
ATAGATTGGGGGAAGGAACA

Downstream 100 bases:

>100_bases
TATAATAAAAGATGAGTGAATTTTCGCTCATCTTTTATTTGTATAGGAGGAATTAGAGTTGTTAAAAGGAATCAATCATC
TTTGTTTTTCAGTATCTAAT

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MKRKVTTIAAVALSTSVLVAGCGNGEKASTTKKEAGKGAADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRL
DKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKATAAEYAFILFDVKNAQKVNKGELPLD
QLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAI
DLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRTVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDN
GSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKGIANHTFGGDFSYKWAYITKK

Sequences:

>Translated_553_residues
MKRKVTTIAAVALSTSVLVAGCGNGEKASTTKKEAGKGAADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRL
DKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKATAAEYAFILFDVKNAQKVNKGELPLD
QLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAI
DLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRTVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDN
GSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKGIANHTFGGDFSYKWAYITKK
>Mature_553_residues
MKRKVTTIAAVALSTSVLVAGCGNGEKASTTKKEAGKGAADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRL
DKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKATAAEYAFILFDVKNAQKVNKGELPLD
QLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAI
DLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRTVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDN
GSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKGIANHTFGGDFSYKWAYITKK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=536, Percent_Identity=30.5970149253731, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1789397, Length=505, Percent_Identity=27.9207920792079, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87081878, Length=543, Percent_Identity=29.097605893186, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1787762, Length=550, Percent_Identity=24.3636363636364, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787052, Length=475, Percent_Identity=21.8947368421053, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62944; Mature: 62944

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKVTTIAAVALSTSVLVAGCGNGEKASTTKKEAGKGAADKQVLNLLETAEIPSMDTSK
CCCCHHHHHHHHHHCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
STDSVSFRAFVNAMEGLYRLDKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCH
QDFVYAWQRTLDKATAAEYAFILFDVKNAQKVNKGELPLDQLGVKAKDDKTLVVELEQPA
HHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCC
PYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
CEEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHEEECCCCCHHHHCCCCCEEECCCCCCC
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMY
CCCCEEEEEEEEEEEEECCCCCEEECCCCCCEEEEEHHHHHHHHCCCCHHHHHCCCCEEE
FLRFNQKNATFKNQKVREAIDLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRT
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHH
VNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDNGSRKKVGDFIKEELEKNLPG
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
IKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
EEEECCCCCCHHHCCCCHHEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHH
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKGIANHTFG
HHHHHCCCCHHCCCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCHHHHHHHCCCCC
GDFSYKWAYITKK
CCCEEEEEEEECC
>Mature Secondary Structure
MKRKVTTIAAVALSTSVLVAGCGNGEKASTTKKEAGKGAADKQVLNLLETAEIPSMDTSK
CCCCHHHHHHHHHHCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
STDSVSFRAFVNAMEGLYRLDKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCH
QDFVYAWQRTLDKATAAEYAFILFDVKNAQKVNKGELPLDQLGVKAKDDKTLVVELEQPA
HHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCC
PYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
CEEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHEEECCCCCHHHHCCCCCEEECCCCCCC
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMY
CCCCEEEEEEEEEEEEECCCCCEEECCCCCCEEEEEHHHHHHHHCCCCHHHHHCCCCEEE
FLRFNQKNATFKNQKVREAIDLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRT
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHH
VNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDNGSRKKVGDFIKEELEKNLPG
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
IKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
EEEECCCCCCHHHCCCCHHEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHH
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKGIANHTFG
HHHHHCCCCHHCCCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCHHHHHHHCCCCC
GDFSYKWAYITKK
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]