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The map label for this gene is livJ [C]

Identifier: 222095577

GI number: 222095577

Start: 1897604

End: 1898821

Strand: Direct

Name: livJ [C]

Synonym: BCQ_1917

Alternate gene names: 222095577

Gene position: 1897604-1898821 (Clockwise)

Preceding gene: 222095576

Following gene: 222095578

Centisome position: 36.39

GC content: 37.6

Gene sequence:

>1218_bases
ATGTCGATGAAAAAACGTAAATGGCTAAAAATGATGGCTACTGGTTGTGTTTTAGGATCGTTATTCATAACGGCAGCTTG
TTCAGGAAAGAAGGCAAGTACAGAAGATGAAAAGACGATTAAAGTAGGGGTTCTTGCTTCATTAACAGGTCCATTAGAAT
CATATGGAAAACAAACAGTGAACGGATTTGAATTAGGATTAGACTATGCAACCGGTGGAACTGGGAAAGTGGAAGGGAAG
AAGATTAAGTTTGTTGTAGAAGATACGGAAACAAAAGCAGAGGTAGCCGTTAAAAAAGCTACGAAGTTATTAGAAGAAGA
GAAAGTCGATTTTTTAGTCGGATCGTCTAGCTCAAGTGATACATTAGCAGTTTTACCATTAGCTGAAGAATATGAAAAAA
TAATGGTCGTAGAACCGGCAGTTGCTGATAGTATTACCGGAAAGAATTGGAATAAGTATGTTTTTAGAACAGGAAGAAGC
TCTTCGCAAGATGCAATTGCAGGAGCAGCTGCAATTGCAAAAAAAGATGTAAAGATTGCGACATTTGCCCCGGATAATGC
ATTTGGCCGTGAAGGGATTGCTGCATTTAAAGCTGGAGCGAAGAAATTAGGTGCAAATATTGTGAACGAGCAATATGCTG
ATACAAATTCGACTGACTTCACTGCAAATATTCAAAATATCATTAGTTCAAAACCAGATTATTTATTTATCGTTTGGGCA
GGGGCGAATTCACCTTGGAAACAGTTGAAAGATATGAATGTAGAAGCGCAAGGTATTAAAATTTCTACTGGTGCACCAGA
TATTCCGGCATTAAAAACGATGGATGCATTAGTAGGAATGCAAGGGTTTTCGGTATATTATCATACACTCCCGAAAAATA
AGGTGAATGATTGGTTAGTAGAAGAACATAAAAAACGATTTAATGGTGCGGTGCCAGATTTATTTACAGCAGGTGGAATG
TCAGCGGCAATTTCTATTGTAGAAGCGTTAAAGAAAACAAAAGGTGATACAGATGTAGATACATTAATTAAGAAAATGGA
AGGAATGGAATTTGATACACCGAAAGGAAAGATGAAGTTTAGAGAAAAGGATCACCAAGCGATGCAGACGCTTTATTCTA
TCACGTTGAAAAAGCAAGATGGTGTTGACTATCCAGTGCCAGTATTAGAGCGAGAATTAACGATGAAAGAGACAGAGCCA
CCAGTTCAAAATAAATAG

Upstream 100 bases:

>100_bases
AAGAGTTTTCCATTACCTATCTTATAGTTAGTCATGATTACGGACAGGGAATTTTGGCGTGTTTGATTCTATCAGAAAGA
AAAGTATGGGGAGGATCACA

Downstream 100 bases:

>100_bases
ACTGAATTTTCTGTTGTTTTTGTATAAAGAGGGGATCCCCTCTTTGTACGTATTTTTTTCATACTTAAAGCTTTAAAGGG
GGGATTTCATGACACATTTG

Product: branched-chain amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSMKKRKWLKMMATGCVLGSLFITAACSGKKASTEDEKTIKVGVLASLTGPLESYGKQTVNGFELGLDYATGGTGKVEGK
KIKFVVEDTETKAEVAVKKATKLLEEEKVDFLVGSSSSSDTLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYVFRTGRS
SSQDAIAGAAAIAKKDVKIATFAPDNAFGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLVEEHKKRFNGAVPDLFTAGGM
SAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKFREKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEP
PVQNK

Sequences:

>Translated_405_residues
MSMKKRKWLKMMATGCVLGSLFITAACSGKKASTEDEKTIKVGVLASLTGPLESYGKQTVNGFELGLDYATGGTGKVEGK
KIKFVVEDTETKAEVAVKKATKLLEEEKVDFLVGSSSSSDTLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYVFRTGRS
SSQDAIAGAAAIAKKDVKIATFAPDNAFGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLVEEHKKRFNGAVPDLFTAGGM
SAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKFREKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEP
PVQNK
>Mature_404_residues
SMKKRKWLKMMATGCVLGSLFITAACSGKKASTEDEKTIKVGVLASLTGPLESYGKQTVNGFELGLDYATGGTGKVEGKK
IKFVVEDTETKAEVAVKKATKLLEEEKVDFLVGSSSSSDTLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYVFRTGRSS
SQDAIAGAAAIAKKDVKIATFAPDNAFGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWAG
ANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLVEEHKKRFNGAVPDLFTAGGMS
AAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKFREKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEPP
VQNK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 44053; Mature: 43922

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKKRKWLKMMATGCVLGSLFITAACSGKKASTEDEKTIKVGVLASLTGPLESYGKQTV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHCC
NGFELGLDYATGGTGKVEGKKIKFVVEDTETKAEVAVKKATKLLEEEKVDFLVGSSSSSD
CHHEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYVFRTGRSSSQDAIAGAAAIAKKDVKIA
CEEEEECHHCCCEEEEECCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEE
TFAPDNAFGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEEE
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLV
CCCCHHHHHHCCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
EEHKKRFNGAVPDLFTAGGMSAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKF
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHH
REKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEPPVQNK
HHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SMKKRKWLKMMATGCVLGSLFITAACSGKKASTEDEKTIKVGVLASLTGPLESYGKQTV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHCC
NGFELGLDYATGGTGKVEGKKIKFVVEDTETKAEVAVKKATKLLEEEKVDFLVGSSSSSD
CHHEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYVFRTGRSSSQDAIAGAAAIAKKDVKIA
CEEEEECHHCCCEEEEECCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEE
TFAPDNAFGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEEE
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLV
CCCCHHHHHHCCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
EEHKKRFNGAVPDLFTAGGMSAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKF
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHH
REKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEPPVQNK
HHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA