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The map label for this gene is gatA [H]

Identifier: 222095572

GI number: 222095572

Start: 1890855

End: 1892465

Strand: Direct

Name: gatA [H]

Synonym: BCQ_1912

Alternate gene names: 222095572

Gene position: 1890855-1892465 (Clockwise)

Preceding gene: 222095571

Following gene: 222095573

Centisome position: 36.26

GC content: 35.69

Gene sequence:

>1611_bases
ATGAAGAAGTGGGTAAAGATAACGTTATCGATCGCTGGAGGTATTGTCCTATTGGCATGTGCCGGAGGGTATTACGTGTA
TAAAAATTATTTTCCAAAGGAACCTGAGCGTATTGTATATGATAAAGAAAGAGTGTTAAAACCGATACATAATCAGCTCA
AAGGAATTAACATAGAAAATGTAAAGATAAAAGAAAAAGAAGTCGTAAATGCGACTGTAGATGAGCTTCAAAAAATGATT
GATGATGGGAAATTATCATATGAAGAATTAACGAGTATTTATCTTTTTAGAATACAAGAACATGATCAAAATGGAATAAC
ATTAAATGCTGTTACAGAGATAAATCCTAATGCGATGGAAGAAGCAAGAAAGTTAGATCAAGAGCGAGGTAGAAACAAAA
AGTCGAATTTATATGGTATTCCTGTCGTTGTAAAAGATAATGTACAAACGTCAAAAGTGATGCCGACTAGTGCAGGAACT
TATGTATTAAAAGATTGGATTGCAGATCAAGACGCAACAATTGTGAAGCAGTTGAAAGAAGAAGGGGCATTTGTTTTAGG
AAAAGCGAATATGTCTGAGTGGGCGAATTATTTATCTTTTACAATGCCAAGCGGGTATAGCGGGAAGAAAGGACAAAATT
TAAATCCGTACGATCCGATTACATTCGATACTTCGGGATCAAGTTCAGGATCTGCTACAGTAGTTACAGCAGATTTTGCA
CCGCTTGCAGTCGGAACAGAAACGACAGGATCGATTGTAGCACCCGCAGCGCAGCAATCAGTAGTTGGGTTACGTCCATC
TTTAGGCATGGTAAGTAGAACAGGAATTATTCCGTTAGCTGAAACTCTTGATACAGCAGGACCTATGGCAAGAACGGTAA
AAGATGCAGCAACGTTATTTAATGCAATGATAGGTTATGACGAAAAAGATGTTATGACAGAAAAAGTGAAAGATAAAGAA
AGAATTGATTATACAAAGGATTTATCAATAGATGGATTAAAAGGGAAAAACATAGGGCTTCTTTTTTCTGTAGACCAACA
AGATGAAAATAGAAAAGCAGTAGCAGAAAAGATTAGAAAAGACCTTCAAGATGCAGGTGCGATATTAACTGAGTATATTC
AGTTAAACAATGGGGGGGTAGATAATTTACAAACATTAGAATATGAGTTCAAGCATAATGTAAACGACTATTTTTCACAA
CAAAAAAATGTGCCGGTAAAATCGTTAAAAGAGATTATAGCGTTTAATAAAAAGGATAGTAATAGACGAATAAAATATGG
ACAAACATTAATTGAGGCGTCTGAAAAATCTGCTATAACGAAAGATGAGTTTGAAAAAGTAGTGCAAACGAGTCAAGAAA
ATGCAAAAAAAGAGCTGAATAGATATTTAGTAGAAAAGGGTTTAGACGCTTTAGTTATGATTAATAACGAGGAAGTTCTT
CTATCCGCTGTAGCTGGCTATCCAGAATTAGCAGTGCCAGCAGGATATGATAACAATGGAGAGCCAGTAGGTGCAGTATT
TGTCGGGAAGCAATTCGGTGAGAAGGAACTGTTCAATATTGGATATGCGTATGAACAGCAGTCTAAAAATAGAAAACCAC
CTAAATTGTAA

Upstream 100 bases:

>100_bases
AATTCTTCATTTTTTCATTTAGCATTTTTATTAAAGAGAGTTATAAAGATAACTCTCTTTTTCAATACAATTGAGCAACT
GTTAGAGTGGAGGAAACAAC

Downstream 100 bases:

>100_bases
AAGGTTAAACGAAAAAAAGATCTCAACTGAAGTAAGTAGGTGGGCTAATATAAATAGAATCTAACAATATAAAAAGAAAA
AAAGGATTCTTCCTAATTAG

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKKWVKITLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLKPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNAVTEINPNAMEEARKLDQERGRNKKSNLYGIPVVVKDNVQTSKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVTADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKNIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTEYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLKEIIAFNKKDSNRRIKYGQTLIEASEKSAITKDEFEKVVQTSQENAKKELNRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL

Sequences:

>Translated_536_residues
MKKWVKITLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLKPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNAVTEINPNAMEEARKLDQERGRNKKSNLYGIPVVVKDNVQTSKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVTADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKNIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTEYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLKEIIAFNKKDSNRRIKYGQTLIEASEKSAITKDEFEKVVQTSQENAKKELNRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
>Mature_536_residues
MKKWVKITLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLKPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNAVTEINPNAMEEARKLDQERGRNKKSNLYGIPVVVKDNVQTSKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVTADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKNIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTEYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLKEIIAFNKKDSNRRIKYGQTLIEASEKSAITKDEFEKVVQTSQENAKKELNRYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=258, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=32.3886639676113, Blast_Score=98, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=229, Percent_Identity=29.6943231441048, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=520, Percent_Identity=22.3076923076923, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=320, Percent_Identity=24.0625, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=221, Percent_Identity=34.841628959276, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319685, Length=177, Percent_Identity=30.5084745762712, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=25.5489021956088, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=24.031007751938, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356731, Length=507, Percent_Identity=22.6824457593688, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=24.1715399610136, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 59461; Mature: 59461

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWVKITLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLKPIHNQLKGINIEN
CCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNAVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEEEEECCCCHHHH
EARKLDQERGRNKKSNLYGIPVVVKDNVQTSKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVTADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKNIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTEYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLKEIIAFNKKDS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCC
NRRIKYGQTLIEASEKSAITKDEFEKVVQTSQENAKKELNRYLVEKGLDALVMINNEEVL
CCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKWVKITLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLKPIHNQLKGINIEN
CCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNAVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEEEEECCCCHHHH
EARKLDQERGRNKKSNLYGIPVVVKDNVQTSKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYDPITFDTSGSSSGSATVVTADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKNIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTEYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLKEIIAFNKKDS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCC
NRRIKYGQTLIEASEKSAITKDEFEKVVQTSQENAKKELNRYLVEKGLDALVMINNEEVL
CCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]