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The map label for this gene is topB-2 [H]

Identifier: 222095556

GI number: 222095556

Start: 1874601

End: 1876745

Strand: Direct

Name: topB-2 [H]

Synonym: BCQ_1896

Alternate gene names: 222095556

Gene position: 1874601-1876745 (Clockwise)

Preceding gene: 222095553

Following gene: 222095557

Centisome position: 35.95

GC content: 37.2

Gene sequence:

>2145_bases
ATGAAATTAATTATTGCCGAGAAACCAGATCAAGGTTTGGCTCTTGTTTCACAGTTTAAATATCGCCGGAAAGATGGATA
TTTAGAAGTAGAAGCGAATGAGTTATTTCCAAATGGAGCGTACTGTACATGGGCAATTGGTCATTTGACGCAGTTATGTA
ATCCAGAACATTATCACGCAGAGTGGAAAAAATGGTCACTTGATACGTTACCGATGATTCCTGAACGATTTCAATTTGAA
GTAACAAAGTCGAAGTATAAACAATTTAACGTTGTGAAACAGCTGTTACATAATCCTCAGGTAACAGAAATTATTCATGC
GGGCGATGCTGGGCGTGAAGGGGAATTGATTGTACGAAATATTATTAACCTTTGCAACGTGCAAAAGCCGATGAAACGTC
TTTGGATTTCATCTTTAACGAAACAAGCTATTTATCAAGGATTTAAAAACTTGCTTGATGAATCAGACACGATTAATACG
TATTATGAAGCATACACAAGATCTTGTGCTGACTGGGTTGTTGGTATGAATGCATCACGTGTGTTTAGTATTTTGTTAAA
GAAAAAAGGAATGAATGATGTATTTTCAGCTGGTCGTGTACAAACACCGACACTCGCGTTAATCGTAAAGCGTGAAAAGG
AAATAGAAAACTTTAAGTCAGAGCCGTTCTGGGAAGTGTTCGCAACCTTTAATATAGAAGGAAAGAAGTATGAGGGGAAA
TGGGAGAAGGATAGTGAATCCCGCTTAAAAGACCCTGATATGGCGAATAAAATTGCGGCATTTTGCCAAGGTAAACCGGC
AGTAGTGAAGGAAATGAAAACGGAGCGTAAAGAATTTCAGCCGCCGCTTTTATTTAACTTATCATCACTGCAAGCAACGG
CAAATAAAGCCTTTAAGTTTTCACCGAAAAAGACGCTTGATATAACGCAAGCACTATATCAAAAAGGGATTGTTTCTTAT
CCTCGTTCGGATTCCAACTATGTTACCCAAGGAGAGGCAGCAACGTTCCCTGATATTTTACAGAAGTTAAGTCAGTTTGA
TGAATATAAAGGTTTATTACCAGCTCCGGTTGAATCGATTATGAATAATAAGCGTTATGTGAATGAAAAGAAAGTAACAG
ATCACTACGCCATTATTCCGACAGAGCAAGTTACAAATCCAAGCAGACTATCAGGTGATGAAAAGAAAATTTACGATATG
ATTGTTAGAAGACTTATAGCAGCTCACTATGAAGTTGCAATCTTTGACTATACAACGATTGTAACGCTTGTAGATGAACG
TGCTGAATTCATTTCAAAAGGAAAACAGCAAATTCAAGAAGGCTGGCGTAAAGTTATTTTCCAAGACGATAAAGATGACG
AAACCATTCTTCCAATTGTAGCTGAAGGTGAAGAAGGAAAAGTTGTAAAAGTGAAAGTGAAAGAGGGAAAAACACAACCA
CCGAAGCGTTATACAGAAGGACAACTTATTACGTTAATGAAAACAGCTGGTAAGTATTTAGAGAATGAAGAGCTGGAGAA
AGTATTAAAGAAAACAGAAGGTTTAGGCACAGAAGCAACTCGCGCTGGAATTATTACGATGCTGAAAGACCGTAAATATA
TAGATGTGAAGAAGAACCAAGTGTATGCGACCGATAAAGGGAAAGTATTAATTACCGCAATCGGTGACAAAATACTAGCT
TCACCAGAAATGACAGCGAAATGGGAGCAGCGCCTTGCAGAAATTGGTGAAGGCACGGCTTCACCAGCTACATTTATGGA
ACAAACGAAAAAGCTATCAGCTAAAATTATTGAAGATGCAGTGGAAATGTCAGAGAAATGGGACTTTACTGGATTGCATG
TTGAATCGATTGAGCGAAAAGGATCGAAATTTACAACAGGTAAAAAGGTTGGTAGCTGTAAAAAATGTGATGGCGATGTG
ATTGATAAGTCAACGTTTTACGGCTGTTCTAATTACAACACGACACAATGTGACTTTACCATCTCGAAGAAGATATTAAG
TAAAACAATTTCGCAAAAGAATATGACAAAGCTATTAAAAGGTGAAAAGACCGATTTAATTAAAGGCTTTAAAAAGGGCG
AGAAAACATTTGATGCGAAATTAGAGTGGAAAGATAATAAGATTAATTTTGTATTTGAGAATTAA

Upstream 100 bases:

>100_bases
GGGACAACCCGCGAATGATAAATAAGATTGTTGTTTTATCATCGCTTCTAAACAGATAGACAATGAAAGTAACAGTGGAA
TACAGATAGGGAGTTATAAA

Downstream 100 bases:

>100_bases
GTTGTTTTAACAAATAAAAATATGATAATTAAATTATATCCTATAGAATAGACGCTTAAAAAAGTCTATTCTATAGGATA
TTTTTTTTGAATAAGTAGTA

Product: DNA topoisomerase iii

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDSESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Sequences:

>Translated_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDSESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
>Mature_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDSESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=673, Percent_Identity=24.3684992570579, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI4507635, Length=458, Percent_Identity=25.764192139738, Blast_Score=106, Evalue=7e-23,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=33.7641357027464, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1787529, Length=518, Percent_Identity=28.1853281853282, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=27.9552715654952, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI32563869, Length=554, Percent_Identity=24.9097472924188, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=629, Percent_Identity=25.9141494435612, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585251, Length=702, Percent_Identity=24.7863247863248, Blast_Score=143, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24640096, Length=495, Percent_Identity=24.040404040404, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81491; Mature: 81491

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDSESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCHHCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
VTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCEEEEEECC
>Mature Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDSESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCHHCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
VTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1329032 [H]