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The map label for this gene is oppA [H]

Identifier: 222095529

GI number: 222095529

Start: 1845841

End: 1847469

Strand: Direct

Name: oppA [H]

Synonym: BCQ_1869

Alternate gene names: 222095529

Gene position: 1845841-1847469 (Clockwise)

Preceding gene: 222095526

Following gene: 222095531

Centisome position: 35.4

GC content: 34.62

Gene sequence:

>1629_bases
ATGAAGCGAAAGAAGAGCCGTTTAATGGTAATGGCACTTGTTACATCTTTATTATTAACAGCTTGTAATAATAAGGAAAA
TAAAAGTGATACAAAGGCAAAAAAACAAGTATTAAATGTAACAGTATCAGAAGAAATTCCTTCTCTTGATACAGCGAAAA
CGATGGATGGTACGTCAGCGCACGTTATGCAAAACATATTTGAAGGATTGTATGCATTAGATGATCAAGATCAGCCGATT
CCAGCAGTAGCAAAATCGTTTAAAAGAAGTGAAGATGGTAAAAAATATACATTCGATTTGCGTAAAGATGCAAAATGGTC
AAATGGGGACAATGTGACAGCGCATGATTTTATGTTTGCGTGGAAACGTGCAATTACCCCTGAAACAGCGTCTCAATATG
CGTCCATGCTCTTTTATGTGAAAAATGCGAAAGAGATTAATAAGGGGACGATGCCTCTTGATGAACTTGGGGTTACAGTT
ATAAATGATTATAAGTTAGAGGTGGAACTAGAACAGCCAATTCCTTATTTTTTACAGTTGTTAGCACTACCTATATATTT
ACCACAGCATGAATCATTTTTGAAAGAACAAGGAAAAAATTATGCATTGGAACCTAATAATCTTATATATAACGGTCCAT
TTGTATTAGAGAAATGGAAGCATGAACAAGAGTTTCAATTAAAGAAGAATGCTACTTATTGGGATGAAAAGAAAGTGAAA
TTAGACGAGATAAACTTTCAAATTGTAAAGGATACAATGACCGCTGTTAATTTATACGAAGCTGGTAATTTGGACCGGGT
GCCTATTAATTCTCAATTTGTAGACAAGTATAAAGGGGATAAGGAGTTACATATGTCGAGCGAACCTGCAATTGCTATGC
TACGTTTTAATGAAAAAAATAGTGCGTTAGCAAATAAGAAAGTGCGTCAGGCTATTTCATTCGCGTTAAATAAAGAGGAT
TTCGTTGCTCATTTTATTAATAACGGAGCAAAACCTGCAACTGGGCTAGTACCAGTTGGGCATATCAATGAAGAAACGGG
AAAAGATTTTAGGGAAGAAAACGGAAACCTTTCTTTATATGATGTACAAAGTGCGAAAAAAAATTGGGAAGAAGCGAAAA
AAGAGCTTGGAGTAGAGCAAGTAAACTTCGAGTTTTTAACATTTGAACAAGATAACGCAAAACGTATGGCAGAATATATA
AAAGGTGATTTAGAAAAGAATTTACAAGGATTAACGATACAAATTAAACAACAGCCATTTAAGCAAAAATTACAGTTAGA
ACAAACAGGTGATTACGATATAACTATGGCAAATTGGGGACCCGACTATAAAGACCCAATTAGTTATTTAGAACTATTTA
CGACGGGGAATCCGAATAATAAAATGAATTATTCTAATGTTCGTTACGATGAATTAATAAAGAAAGCGAAAACGGATTTT
GTACTAGATACGGAAAACCGATGGGAAGCGTTGCGAGAAGCCGAGCAGGTATTATTAGAAGATGCTGCGGTGGTGCCGCT
TTATCATATTGGCTCAGCATATGTACAAAAGGATTATGTAAAAGGAATTGAAAAGCATCAATTTGGTGGTATTTATACTT
ATAAGAATGCTTATGTTGCAAATGAATAA

Upstream 100 bases:

>100_bases
TGAACGAATAAATATTCAAAAGAATGTCGCATCTTTCTGGTAGTATTTCTGCTATAATATATTACGCTTTATAATATTTT
ATATTTAAGGAGAACAACTC

Downstream 100 bases:

>100_bases
ATACAAAAAACCTTACTTTTTTGAAGTAAGGTTTTTTTACACATGTATTCTATTAAAACGATTTTAATAAAGAATAAAAA
TTCGTATCATAGGGAATGTC

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKRKKSRLMVMALVTSLLLTACNNKENKSDTKAKKQVLNVTVSEEIPSLDTAKTMDGTSAHVMQNIFEGLYALDDQDQPI
PAVAKSFKRSEDGKKYTFDLRKDAKWSNGDNVTAHDFMFAWKRAITPETASQYASMLFYVKNAKEINKGTMPLDELGVTV
INDYKLEVELEQPIPYFLQLLALPIYLPQHESFLKEQGKNYALEPNNLIYNGPFVLEKWKHEQEFQLKKNATYWDEKKVK
LDEINFQIVKDTMTAVNLYEAGNLDRVPINSQFVDKYKGDKELHMSSEPAIAMLRFNEKNSALANKKVRQAISFALNKED
FVAHFINNGAKPATGLVPVGHINEETGKDFREENGNLSLYDVQSAKKNWEEAKKELGVEQVNFEFLTFEQDNAKRMAEYI
KGDLEKNLQGLTIQIKQQPFKQKLQLEQTGDYDITMANWGPDYKDPISYLELFTTGNPNNKMNYSNVRYDELIKKAKTDF
VLDTENRWEALREAEQVLLEDAAVVPLYHIGSAYVQKDYVKGIEKHQFGGIYTYKNAYVANE

Sequences:

>Translated_542_residues
MKRKKSRLMVMALVTSLLLTACNNKENKSDTKAKKQVLNVTVSEEIPSLDTAKTMDGTSAHVMQNIFEGLYALDDQDQPI
PAVAKSFKRSEDGKKYTFDLRKDAKWSNGDNVTAHDFMFAWKRAITPETASQYASMLFYVKNAKEINKGTMPLDELGVTV
INDYKLEVELEQPIPYFLQLLALPIYLPQHESFLKEQGKNYALEPNNLIYNGPFVLEKWKHEQEFQLKKNATYWDEKKVK
LDEINFQIVKDTMTAVNLYEAGNLDRVPINSQFVDKYKGDKELHMSSEPAIAMLRFNEKNSALANKKVRQAISFALNKED
FVAHFINNGAKPATGLVPVGHINEETGKDFREENGNLSLYDVQSAKKNWEEAKKELGVEQVNFEFLTFEQDNAKRMAEYI
KGDLEKNLQGLTIQIKQQPFKQKLQLEQTGDYDITMANWGPDYKDPISYLELFTTGNPNNKMNYSNVRYDELIKKAKTDF
VLDTENRWEALREAEQVLLEDAAVVPLYHIGSAYVQKDYVKGIEKHQFGGIYTYKNAYVANE
>Mature_542_residues
MKRKKSRLMVMALVTSLLLTACNNKENKSDTKAKKQVLNVTVSEEIPSLDTAKTMDGTSAHVMQNIFEGLYALDDQDQPI
PAVAKSFKRSEDGKKYTFDLRKDAKWSNGDNVTAHDFMFAWKRAITPETASQYASMLFYVKNAKEINKGTMPLDELGVTV
INDYKLEVELEQPIPYFLQLLALPIYLPQHESFLKEQGKNYALEPNNLIYNGPFVLEKWKHEQEFQLKKNATYWDEKKVK
LDEINFQIVKDTMTAVNLYEAGNLDRVPINSQFVDKYKGDKELHMSSEPAIAMLRFNEKNSALANKKVRQAISFALNKED
FVAHFINNGAKPATGLVPVGHINEETGKDFREENGNLSLYDVQSAKKNWEEAKKELGVEQVNFEFLTFEQDNAKRMAEYI
KGDLEKNLQGLTIQIKQQPFKQKLQLEQTGDYDITMANWGPDYKDPISYLELFTTGNPNNKMNYSNVRYDELIKKAKTDF
VLDTENRWEALREAEQVLLEDAAVVPLYHIGSAYVQKDYVKGIEKHQFGGIYTYKNAYVANE

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=524, Percent_Identity=30.3435114503817, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI87081878, Length=534, Percent_Identity=28.0898876404494, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1789397, Length=526, Percent_Identity=26.9961977186312, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789966, Length=476, Percent_Identity=26.0504201680672, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787762, Length=556, Percent_Identity=22.841726618705, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787052, Length=343, Percent_Identity=27.1137026239067, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787551, Length=572, Percent_Identity=20.4545454545455, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789887, Length=324, Percent_Identity=25, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62205; Mature: 62205

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKKSRLMVMALVTSLLLTACNNKENKSDTKAKKQVLNVTVSEEIPSLDTAKTMDGTSA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEHHCCCCCCCCCCCCCCHH
HVMQNIFEGLYALDDQDQPIPAVAKSFKRSEDGKKYTFDLRKDAKWSNGDNVTAHDFMFA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
WKRAITPETASQYASMLFYVKNAKEINKGTMPLDELGVTVINDYKLEVELEQPIPYFLQL
HHHHCCCHHHHHHHHHHHHHHCHHHCCCCCCCHHHCCEEEEECEEEEEEECCCCHHHHHH
LALPIYLPQHESFLKEQGKNYALEPNNLIYNGPFVLEKWKHEQEFQLKKNATYWDEKKVK
HHHHHCCCCHHHHHHHCCCCEEECCCCEEECCCCHHHHCCCCHHHHHHCCCCCCCCCCEE
LDEINFQIVKDTMTAVNLYEAGNLDRVPINSQFVDKYKGDKELHMSSEPAIAMLRFNEKN
EECCCEEEEEHHHHHHEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCEEEEEECCCC
SALANKKVRQAISFALNKEDFVAHFINNGAKPATGLVPVGHINEETGKDFREENGNLSLY
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHCCHHHHCCCCEEEE
DVQSAKKNWEEAKKELGVEQVNFEFLTFEQDNAKRMAEYIKGDLEKNLQGLTIQIKQQPF
ECHHHHHHHHHHHHHCCCEEECEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCH
KQKLQLEQTGDYDITMANWGPDYKDPISYLELFTTGNPNNKMNYSNVRYDELIKKAKTDF
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHCCCE
VLDTENRWEALREAEQVLLEDAAVVPLYHIGSAYVQKDYVKGIEKHQFGGIYTYKNAYVA
EEECHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEECCEEEC
NE
CC
>Mature Secondary Structure
MKRKKSRLMVMALVTSLLLTACNNKENKSDTKAKKQVLNVTVSEEIPSLDTAKTMDGTSA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEHHCCCCCCCCCCCCCCHH
HVMQNIFEGLYALDDQDQPIPAVAKSFKRSEDGKKYTFDLRKDAKWSNGDNVTAHDFMFA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
WKRAITPETASQYASMLFYVKNAKEINKGTMPLDELGVTVINDYKLEVELEQPIPYFLQL
HHHHCCCHHHHHHHHHHHHHHCHHHCCCCCCCHHHCCEEEEECEEEEEEECCCCHHHHHH
LALPIYLPQHESFLKEQGKNYALEPNNLIYNGPFVLEKWKHEQEFQLKKNATYWDEKKVK
HHHHHCCCCHHHHHHHCCCCEEECCCCEEECCCCHHHHCCCCHHHHHHCCCCCCCCCCEE
LDEINFQIVKDTMTAVNLYEAGNLDRVPINSQFVDKYKGDKELHMSSEPAIAMLRFNEKN
EECCCEEEEEHHHHHHEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCEEEEEECCCC
SALANKKVRQAISFALNKEDFVAHFINNGAKPATGLVPVGHINEETGKDFREENGNLSLY
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHCCHHHHCCCCEEEE
DVQSAKKNWEEAKKELGVEQVNFEFLTFEQDNAKRMAEYIKGDLEKNLQGLTIQIKQQPF
ECHHHHHHHHHHHHHCCCEEECEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCH
KQKLQLEQTGDYDITMANWGPDYKDPISYLELFTTGNPNNKMNYSNVRYDELIKKAKTDF
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHCCCE
VLDTENRWEALREAEQVLLEDAAVVPLYHIGSAYVQKDYVKGIEKHQFGGIYTYKNAYVA
EEECHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEECCEEEC
NE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]