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The map label for this gene is ilvB [H]

Identifier: 222095511

GI number: 222095511

Start: 1826220

End: 1827935

Strand: Direct

Name: ilvB [H]

Synonym: BCQ_1851

Alternate gene names: 222095511

Gene position: 1826220-1827935 (Clockwise)

Preceding gene: 222095510

Following gene: 222095512

Centisome position: 35.02

GC content: 39.86

Gene sequence:

>1716_bases
ATGGAGCAACAGTATACAACATATGAGAAATTACAGTGTGAAGATGTGACAGGAGCTGGACACGTTATTCAATGCTTGAA
GAATTTAGGCGTAACGACTGTTTTCGGTTATCCGGGCGGAGCGATTTTGCCAGTGTACGATGCGTTATACGGAAGTGGTT
TAAAGCACATTTTAACTCGTCATGAACAAGCTGCTATTCATGCGGCAGAAGGATATGCGAGAGCTTCTGGAAAGGTCGGG
GTAGTCTTTGCTACCTCTGGCCCAGGGGCGACAAATCTAGTTACGGGCTTAGCAGATGCTTATATGGATTCGATTCCTTT
AGTTGTCATTACAGGGCAAGTTGCAACGCCTTTAATTGGTAAAGATGGATTTCAAGAAGCGGATGTTGTCGGAATTACAG
TACCTGTTACGAAGCATAATTACCAAGTTCGTGATGTAAATCATGTATCACGAATTGTGCAAGAAGCTTTTTACATCGCC
AAAAGCGGACGACCGGGACCAGTATTAATCGATATTCCAAAAGATGTTCAAAATGCAAAAGTCACCAGTTTCTTTAATGA
AGAAGTTGATATTCCGGGGTACAAACCAGAACTCGTACCAGACAGCATGAAACTTAGAGAGGTAGCGAAAGAAATTTCAA
AAGCAAAACGTCCACTTCTTTATATTGGAGGAGGTGTCATTCATTCAGATGGATCTGATGAACTGATCAAATTTGCAAGA
GAAAATCGTATTCCAGTCGTGTCAACTTTAATGGGACTAGGTGCGTATCCGCCGGGAGATTCATTATTTTTAGGAATGCT
CGGTATGCATGGAACGTATGCTGCAAACATGGCAGTAACAGAATGTGACTTATTACTTGCTTTAGGTGTTCGTTTTGATG
ACCGTGTAACAGGAAAATTAGAACTCTTTTCTCCGCATTCGAAAAAGGTACATATTGATATAGATCCTTCGGAGTTTCAT
AAAAATGTAACTGTGGAATATCCAGTTGTTGGAGATGTGAAAAAAGCGTTACACATGTTGTTACATATGCCGATTTGTAC
ACAGACAGATGAATGGATTACGAAAACCGAGGAATGGAAAGCGGAATACCCTCTTTCCTATATTCAAAAAGAAAGTGAGT
TAAAACCACAGCATGTTATTAGCTTAGTAAGTGAATTAACGAATGGTGAAGCGATTGTTACGACAGAAGTAGGACAGCAT
CAAATGTGGGCTGCGCATTTTTATAAAGCGAAAAACCCGCGGACTTTTCTAACATCAGGTGGATTAGGAACGATGGGATT
TGGCTTCCCGGCGGCAGTTGGTGCTCAGCTTGCAAAAGAAGAAGAACTCGTTATTTGTATTGCAGGTGATGCTTCTTTTC
AAATGAATATTCAAGAACTACAAACAATTGCTGAAAATAACATCCCTGTAAAAGTATTTATTATAAACAATAAATTTTTA
GGGATGGTAAGGCAATGGCAAGAAATGTTTTATGAAAATCGATTATCAGAGTCGAAAATTGGATCGCCAGATTTTGTGAA
AGTGGCAGAAGCTTATGGAGTGAAAGGGTTAAGAGCGACAAATTCAACTGAGGCGAAACAAGTGATGTTAGAAGCATTTG
CTCATGAAGGACCTGTCGTAGTTGATTTTTGTGTAGAAGAAGGTGAAAACGTATTTCCGATGGTTCCACCAAATAAAGGG
AATAACGAAATGATTATGAAGAGGTGGGAAGAATGA

Upstream 100 bases:

>100_bases
CAATTGACAGAGGAATTTAAAAAGTTAACGAGGGAAAGAGGAGTACGTGTTCCAGGATTGGCGGAAAGCTTACTGTAGGT
AGGGAGAGGAGTTAATTAAA

Downstream 100 bases:

>100_bases
GTCATACTTTTTCATTAGTTATCGATAACGATCCAAATGTCTTATTACGCATAAGTGGAATTTTTGCTCGGCGTGGTTAT
TATATTTCTTCATTAAATTT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MEQQYTTYEKLQCEDVTGAGHVIQCLKNLGVTTVFGYPGGAILPVYDALYGSGLKHILTRHEQAAIHAAEGYARASGKVG
VVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNHVSRIVQEAFYIA
KSGRPGPVLIDIPKDVQNAKVTSFFNEEVDIPGYKPELVPDSMKLREVAKEISKAKRPLLYIGGGVIHSDGSDELIKFAR
ENRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSEFH
KNVTVEYPVVGDVKKALHMLLHMPICTQTDEWITKTEEWKAEYPLSYIQKESELKPQHVISLVSELTNGEAIVTTEVGQH
QMWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAVGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFL
GMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPNKG
NNEMIMKRWEE

Sequences:

>Translated_571_residues
MEQQYTTYEKLQCEDVTGAGHVIQCLKNLGVTTVFGYPGGAILPVYDALYGSGLKHILTRHEQAAIHAAEGYARASGKVG
VVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNHVSRIVQEAFYIA
KSGRPGPVLIDIPKDVQNAKVTSFFNEEVDIPGYKPELVPDSMKLREVAKEISKAKRPLLYIGGGVIHSDGSDELIKFAR
ENRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSEFH
KNVTVEYPVVGDVKKALHMLLHMPICTQTDEWITKTEEWKAEYPLSYIQKESELKPQHVISLVSELTNGEAIVTTEVGQH
QMWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAVGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFL
GMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPNKG
NNEMIMKRWEE
>Mature_571_residues
MEQQYTTYEKLQCEDVTGAGHVIQCLKNLGVTTVFGYPGGAILPVYDALYGSGLKHILTRHEQAAIHAAEGYARASGKVG
VVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNHVSRIVQEAFYIA
KSGRPGPVLIDIPKDVQNAKVTSFFNEEVDIPGYKPELVPDSMKLREVAKEISKAKRPLLYIGGGVIHSDGSDELIKFAR
ENRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSEFH
KNVTVEYPVVGDVKKALHMLLHMPICTQTDEWITKTEEWKAEYPLSYIQKESELKPQHVISLVSELTNGEAIVTTEVGQH
QMWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAVGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFL
GMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPNKG
NNEMIMKRWEE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=591, Percent_Identity=25.5499153976311, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=24.4604316546763, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=46.0732984293194, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=42.7304964539007, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786717, Length=553, Percent_Identity=34.7197106690778, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=27.1062271062271, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1788716, Length=575, Percent_Identity=22.9565217391304, Blast_Score=139, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=554, Percent_Identity=28.158844765343, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=554, Percent_Identity=28.158844765343, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=24.9560632688928, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=45.8333333333333, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320816, Length=481, Percent_Identity=25.7796257796258, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320123, Length=516, Percent_Identity=22.093023255814, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=495, Percent_Identity=23.4343434343434, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922626, Length=470, Percent_Identity=26.5957446808511, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62703; Mature: 62703

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQYTTYEKLQCEDVTGAGHVIQCLKNLGVTTVFGYPGGAILPVYDALYGSGLKHILTR
CCCCCCCHHHEEECCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHH
HEQAAIHAAEGYARASGKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIG
HHHHHEEHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
KDGFQEADVVGITVPVTKHNYQVRDVNHVSRIVQEAFYIAKSGRPGPVLIDIPKDVQNAK
CCCCCCCCEEEEEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCE
VTSFFNEEVDIPGYKPELVPDSMKLREVAKEISKAKRPLLYIGGGVIHSDGSDELIKFAR
EHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEECCCHHHHHHHHH
ENRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKL
HCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEE
ELFSPHSKKVHIDIDPSEFHKNVTVEYPVVGDVKKALHMLLHMPICTQTDEWITKTEEWK
EEECCCCCEEEEEECHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHC
AEYPLSYIQKESELKPQHVISLVSELTNGEAIVTTEVGQHQMWAAHFYKAKNPRTFLTSG
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECC
GLGTMGFGFPAAVGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFL
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCHHH
GMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEE
VDFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEEECCCCCEECCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MEQQYTTYEKLQCEDVTGAGHVIQCLKNLGVTTVFGYPGGAILPVYDALYGSGLKHILTR
CCCCCCCHHHEEECCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHH
HEQAAIHAAEGYARASGKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIG
HHHHHEEHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
KDGFQEADVVGITVPVTKHNYQVRDVNHVSRIVQEAFYIAKSGRPGPVLIDIPKDVQNAK
CCCCCCCCEEEEEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCE
VTSFFNEEVDIPGYKPELVPDSMKLREVAKEISKAKRPLLYIGGGVIHSDGSDELIKFAR
EHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEECCCHHHHHHHHH
ENRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKL
HCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEE
ELFSPHSKKVHIDIDPSEFHKNVTVEYPVVGDVKKALHMLLHMPICTQTDEWITKTEEWK
EEECCCCCEEEEEECHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHC
AEYPLSYIQKESELKPQHVISLVSELTNGEAIVTTEVGQHQMWAAHFYKAKNPRTFLTSG
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECC
GLGTMGFGFPAAVGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFL
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCHHH
GMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEE
VDFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEEECCCCCEECCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]