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The map label for this gene is amiB [H]

Identifier: 222095485

GI number: 222095485

Start: 1795755

End: 1796999

Strand: Direct

Name: amiB [H]

Synonym: BCQ_1825

Alternate gene names: 222095485

Gene position: 1795755-1796999 (Clockwise)

Preceding gene: 222095483

Following gene: 222095486

Centisome position: 34.44

GC content: 35.02

Gene sequence:

>1245_bases
ATGAAAAATAGAATAATCGCAGCAGGAGTAATTGCAGCTAGTATATTATCTTATTCATCTAGTAGTTTCGCGCAAACGAA
AAAATTTCCAGATGTCCCAGCTAAACATTGGGCAGAAGACTCTATTTACTATTTAGTAGAGAAAGGTGCAGTTACAGGTA
ACGAAAAAGGAATGTTTGAGCCAGGAAAAGAAATCACTCGTGCAGAAGCAGCTACAATGATGGCTCAAATCTTAAACTTA
CCAATCGATAAAAATGCTAAACCATCTTTCGGTGATTCTCAAAACCAATGGTATACTCCATTCATCGCAGCTGTAGAAAA
AGCTGGCGTTGTAAAAGGGAAAGGAGCTGGCGTATTCGATCCAACTGGGAAAATTGACCGAGTTTCAATGGCCTCTCTTA
TTGTAGAAGCTTACAAATTAGATTCTAAAGTAAATGGTACTCCAGCAACTAAATTTAGTGATCTAGAAAAAAGTTGGGGT
AAAGCAAAAGCTAACATTCTAGTTGAATTAGGGATTTCTATTGGAACTGGTGACAAATGGGAGCCCGAAAAAATTCTAAC
TAAAGCAGAGGCAGCTCAATTTATAAAAAAGGCAGATTCTCTTAAAATAGGTAACCCTTTAGTAGAAAAGGTAGTTATTA
TTGATCCTGGTCATGGAGGATTTGATCCAGGGAATCCAGGGCAAGGTGTAGAGGAAAGTAAAATCGTATTTGATACATCT
TTAAGATTACAGAAGTTGTTAGAAAAAAATACACCTTTAAAAGCTTTATTAACACGTGAAGAGAACGGAAATCCAGGAAG
TAACAAAAATGAATCTCTTGCGAATCGAGTGAAATTTGGTCAAGAGAACAATGCAGATATATTTGTAAGCATTCATGCAA
ATTCTTCTGAAAATCATGATGGTCATGGAACGGAAACGTATTATTATAAAAAATCCAAGCGTGGAGAAGAGACACAGATT
GAAAAGGATAGTGAAGTGTTAGCAAAGAAAATTCAAAATAGAGTAGTAGAAGCACTACATACAAGAGATAGAAATATAAA
AGACAATCACTCACTTTATGTAGTAAATAATAATACAGTACCAGCAGTTTTAACAGAGCTTGCCTTTATTGATAATGATA
TAGATAATGGTAAATTAGCTACTGAATCAGGAAGACAAATTGCTGCTGAGGCAGTTTATGCTGGGATTTTAGATTATTAT
GAGTGGAAAGGTTTCGATGTTGAAAAATATCGTTTAGCTAAATAA

Upstream 100 bases:

>100_bases
AATACAAAATTATACAATTTTCAATTGACTATTACTAATTTTCAGAACTATAATTAGTCTGTTACATCAATACATCGTAC
TTGAGAGGGGAATATATTCT

Downstream 100 bases:

>100_bases
CAATATTGATTGCTAAGTCCATCGTCTACATAGAGATGGGCTTTTTATGTGATTTTATGTAATTTTGTATCGAAATAAAA
AGATATATCGTATTATGTTG

Product: s-layer protein and N-acetylmuramoyl-l-alanine amidase fusion protein

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: Surface array protein; Surface layer protein [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKNRIIAAGVIAASILSYSSSSFAQTKKFPDVPAKHWAEDSIYYLVEKGAVTGNEKGMFEPGKEITRAEAATMMAQILNL
PIDKNAKPSFGDSQNQWYTPFIAAVEKAGVVKGKGAGVFDPTGKIDRVSMASLIVEAYKLDSKVNGTPATKFSDLEKSWG
KAKANILVELGISIGTGDKWEPEKILTKAEAAQFIKKADSLKIGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSENHDGHGTETYYYKKSKRGEETQI
EKDSEVLAKKIQNRVVEALHTRDRNIKDNHSLYVVNNNTVPAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYY
EWKGFDVEKYRLAK

Sequences:

>Translated_414_residues
MKNRIIAAGVIAASILSYSSSSFAQTKKFPDVPAKHWAEDSIYYLVEKGAVTGNEKGMFEPGKEITRAEAATMMAQILNL
PIDKNAKPSFGDSQNQWYTPFIAAVEKAGVVKGKGAGVFDPTGKIDRVSMASLIVEAYKLDSKVNGTPATKFSDLEKSWG
KAKANILVELGISIGTGDKWEPEKILTKAEAAQFIKKADSLKIGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSENHDGHGTETYYYKKSKRGEETQI
EKDSEVLAKKIQNRVVEALHTRDRNIKDNHSLYVVNNNTVPAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYY
EWKGFDVEKYRLAK
>Mature_414_residues
MKNRIIAAGVIAASILSYSSSSFAQTKKFPDVPAKHWAEDSIYYLVEKGAVTGNEKGMFEPGKEITRAEAATMMAQILNL
PIDKNAKPSFGDSQNQWYTPFIAAVEKAGVVKGKGAGVFDPTGKIDRVSMASLIVEAYKLDSKVNGTPATKFSDLEKSWG
KAKANILVELGISIGTGDKWEPEKILTKAEAAQFIKKADSLKIGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSENHDGHGTETYYYKKSKRGEETQI
EKDSEVLAKKIQNRVVEALHTRDRNIKDNHSLYVVNNNTVPAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYY
EWKGFDVEKYRLAK

Specific function: The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall, S-layer [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=272, Percent_Identity=27.5735294117647, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003343
- InterPro:   IPR008964
- InterPro:   IPR001119 [H]

Pfam domain/function: PF02368 Big_2; PF00395 SLH [H]

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 45410; Mature: 45410

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRIIAAGVIAASILSYSSSSFAQTKKFPDVPAKHWAEDSIYYLVEKGAVTGNEKGMFE
CCCCEEEHHHHHHHHHHCCCCHHHHHCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCC
PGKEITRAEAATMMAQILNLPIDKNAKPSFGDSQNQWYTPFIAAVEKAGVVKGKGAGVFD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECHHHHHHHHCCCEECCCCCCCC
PTGKIDRVSMASLIVEAYKLDSKVNGTPATKFSDLEKSWGKAKANILVELGISIGTGDKW
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHEEEEEEECCEECCCCCC
EPEKILTKAEAAQFIKKADSLKIGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHH
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSENHD
HHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GHGTETYYYKKSKRGEETQIEKDSEVLAKKIQNRVVEALHTRDRNIKDNHSLYVVNNNTV
CCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCH
PAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYYEWKGFDVEKYRLAK
HHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MKNRIIAAGVIAASILSYSSSSFAQTKKFPDVPAKHWAEDSIYYLVEKGAVTGNEKGMFE
CCCCEEEHHHHHHHHHHCCCCHHHHHCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCC
PGKEITRAEAATMMAQILNLPIDKNAKPSFGDSQNQWYTPFIAAVEKAGVVKGKGAGVFD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECHHHHHHHHCCCEECCCCCCCC
PTGKIDRVSMASLIVEAYKLDSKVNGTPATKFSDLEKSWGKAKANILVELGISIGTGDKW
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHEEEEEEECCEECCCCCC
EPEKILTKAEAAQFIKKADSLKIGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHH
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSENHD
HHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GHGTETYYYKKSKRGEETQIEKDSEVLAKKIQNRVVEALHTRDRNIKDNHSLYVVNNNTV
CCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCH
PAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYYEWKGFDVEKYRLAK
HHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836294; 12721629 [H]