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The map label for this gene is asnO [H]

Identifier: 222095430

GI number: 222095430

Start: 1735748

End: 1737571

Strand: Reverse

Name: asnO [H]

Synonym: BCQ_1770

Alternate gene names: 222095430

Gene position: 1737571-1735748 (Counterclockwise)

Preceding gene: 222095433

Following gene: 222095428

Centisome position: 33.32

GC content: 35.91

Gene sequence:

>1824_bases
ATGTGTGGAATTACAGGTTGGGTAAATTGGAATAAAGATCTTTCCAATGAACATGTTATTTTAAAAAAGATGGCAAATCG
TATTCAGCATCGCGGTCCGGATGCTGAAGGATTTTGGTTTTCACCTCGCGCGGCTTTTGCACACCGCCGTTTAATTGTAA
TTGATCCAGAAGGTGGGACACAGCCGAAGACATTTCGTGCTGGTGATTATACGTATGCTCTTACTTATAATGGAGAAATT
TATAATTTCCGTGAGCTGAGAGAACAACTTAGGAAATGTGGTCATACATTTGAAACGCATTCAGATACAGAAGTGTTACT
ACATGCTTATTTAGAATGGAAAGAAGACTGCGTACAACATTTAAACGGCATTTTCGCTTTCGCCTTATGGGATGATCAGA
AGCAACAACTGTTTTTAGCACGTGATCATTTAGGCGTAAAACCACTCTTTTATACAGAAAGAAATGACAGTATTATTTTC
GGATCTGAAATTAAAGCATTATTAGCTCATCCATCTGTCCCTGCTGAAATTGATACGGATGGCATAAATGAAATATTTGG
ATTAGGGTTATTTCGAACTCCAGGATGTGGCGTCTTTAAACATATTCAAGAAGTGCGTGCTGGACATTCTATAACGTTTA
CACGTGATAAAAAAGTAGTTACGAAGTATTGGAATTTAGAAAGTAAGGTCCATACAGATTCTATAGAAGATACATCATCA
CATATTTTATCTATTTTACAAGATACAGTAAAAAGACAATTAATTGCCGATGTCCCTCTCGTCTGCATGTTATCAGGAGG
ATTAGATTCTAGCGGTATTACTGCACTAGCAGGAAAAGAATTTGCTGCTGAAAATAAAACACTTCATACGTATTCCGTTG
ACTTTGTAAATAGCGCAAAAGATTTCGAGCTGACATTTGCTCGCACTGGTTTAGACGCTCCTTGGGTAAAACGCGTTTCT
GAACATGTAGGGACATCTCATCATGATATTATTGTGAATGCTGAAGAATTAGCTAATCACTTATTCGTTCCCCTCCGTGC
AAAAGATTTACCGAGTGCTGGCGAAATGGAAACTTCACTATATTTACTATTTTGCGAAATGAAAAAAGATGCGACCGTAG
CTTTATCGGGAGAATCTGCTGATGAAGTATTCGGTGGATATCCTTGGTTTCATCAAGAAGAATTACTATATGTAGATAAA
TTTCCTTGGCTAACAAATTGGAAAAATACATCTCCTCTTCTACTACATGAAATAAGTAACCAATGTAATCTAGAAAACTA
TATTGATACACGATTCCAAGAAGCGATACTTGAAGTACCTACTCTTGAAGGTGAAAGTAAAAAATCCGCGAAACAACGTC
AAATGTTTTATTTATTTTTAACGAGATTTCTTCCGTTCTTACTTGACCGTAAAGACCGTATGAGTATGGCTGTAGGATTT
GAAGTTCGTGTACCGTTTTGCGATTATAGATTAGTTGAATATTTATGGAACGTTCCTTTCAACATAAAAAGCATTGATAA
TATTGAAAAAGGCATTTTACGTAGAGCACTGAAACCTGCTTTACCTGACGATGTACGTAATAGAAGAAAAAGTGCTTATC
CAACTTCACAAGACCCACATTATTTACAAACAATCCGGAATTTAACACTTGAAATGTGTAGTAATAAAAACAACCCTATT
TTCTCACTTATTAATCATTCCACATTACTTGCTATTGCCGATCAGAGTAATAAAGAAATTAACAATTTCGAAGCAAGAAG
TGCTATGGAATACATGCTACAAGTAAATGAATGGTTAAAAACGTATCACATCCATATTGCTTAA

Upstream 100 bases:

>100_bases
AATTTGATTCATGTAGTACACTATTTATATAGTCATTCTTTAACTTAGTGTACATTTTATTTCGTTAACCTACTATTTAA
AATTAGGAGATGAGATGTGT

Downstream 100 bases:

>100_bases
AAAAAGAGTTCAAGGATAAAAAACCTTGAACTCCTTTTATATTAATTAGCACATCGTTTATTTTCAGTGTCAATGGACCA
TCCAATAATTGTGGCACAAT

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGTQPKTFRAGDYTYALTYNGEI
YNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQQLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDTDGINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEISNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLEMCSNKNNPI
FSLINHSTLLAIADQSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA

Sequences:

>Translated_607_residues
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGTQPKTFRAGDYTYALTYNGEI
YNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQQLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDTDGINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEISNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLEMCSNKNNPI
FSLINHSTLLAIADQSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA
>Mature_607_residues
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGTQPKTFRAGDYTYALTYNGEI
YNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQQLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDTDGINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEISNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLEMCSNKNNPI
FSLINHSTLLAIADQSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=554, Percent_Identity=27.7978339350181, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI168229252, Length=554, Percent_Identity=27.7978339350181, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI168229250, Length=554, Percent_Identity=27.7978339350181, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI296010848, Length=528, Percent_Identity=27.6515151515151, Blast_Score=158, Evalue=2e-38,
Organism=Homo sapiens, GI296010852, Length=454, Percent_Identity=25.9911894273128, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI296010850, Length=454, Percent_Identity=25.9911894273128, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1786889, Length=411, Percent_Identity=30.4136253041363, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=561, Percent_Identity=26.3814616755793, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=561, Percent_Identity=26.3814616755793, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25147560, Length=326, Percent_Identity=26.3803680981595, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=431, Percent_Identity=28.3062645011601, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6325403, Length=420, Percent_Identity=29.0476190476191, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45553209, Length=378, Percent_Identity=29.1005291005291, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69687; Mature: 69687

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQQLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDTD
HCCEEEEEEECCCHHHEEEEHHCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLHEISN
EEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
QCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPH
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCH
YLQTIRNLTLEMCSNKNNPIFSLINHSTLLAIADQSNKEINNFEARSAMEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC
>Mature Secondary Structure
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREQLRKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQQLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDTD
HCCEEEEEEECCCHHHEEEEHHCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLHEISN
EEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
QCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPH
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCH
YLQTIRNLTLEMCSNKNNPIFSLINHSTLLAIADQSNKEINNFEARSAMEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]