Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 222095199

GI number: 222095199

Start: 1514786

End: 1515910

Strand: Direct

Name: dacB [H]

Synonym: BCQ_1539

Alternate gene names: 222095199

Gene position: 1514786-1515910 (Clockwise)

Preceding gene: 222095198

Following gene: 222095200

Centisome position: 29.05

GC content: 36.62

Gene sequence:

>1125_bases
ATGAGACGAATTTGTGTAATCATTACGCTTCTTATTATGTACGCAAGCGTTATACCAATTCCTACATATGCAAAGATGAC
CAGCAATGTTAGTGCTCGAAATGCTGTATTAATGGAGCAACAGTCAGGTCGCGTATTATACGGAAAAGCAGAACATGAGC
CGCAAAAGATTGCTAGTATAACAAAAATTATGACTGCCTTGTTAGCTGCTGAGTCAGGAAAAATGAAAGAAATGGTCTCG
GTTAGTAATGAAGCAGTGAGAGTAGAAGGCTCAGCAATTTATTTAAAGCCTGGACAGAAAGTAAAATTAGAGGATTTAGT
ATATGGACTCATGCTTAGATCTGGTAATGATGCAGCACAAGTAATTGCTGAAAATGTAGGAGGGAGTATAGAAGGGTTTG
TATATTTAATGAATGAAAAGGCGAAACAAATTGGAATGAAAGATACTCACTTTTCAAACCCACATGGTTTGGATGGAGAT
GGATCCCATTATTCGTCGGCCTATGATATGGCACTTTTAACAAGGTATGCAATGGGGAACGAAACCTTTAAGAAGATTTT
TGGGACAAAAACGTATAAATCAGATTCGTGGGATTATCCGTGGAAAAACAAACATAAACTTGTGACGTCTTATTATGAAT
TTGCAACAGGAGGAAAAACGGGTTTTACGAAGAAAGCAGGCCGGACGCTTGTTACAACAGCATCAAAAGATGGACTAGAT
CTAATTGTCGTAACTTTGAGCGCTTCTAGTGATTGGGATGATCATATGAATTTATTTGATAAAGGTTTTGACCGTTTCGA
ACAAACAAAAGTTTTAGGACAAGGAGCACTTGCTGAAATAACCGAAAAGAAATACGCCAATCATGTTTACACGAAAAATA
GTTTTTCAGTACCTTTAACTGAAGAAGAAAAAAAGAGCGTAGTATTAAAAGTTGAACTAGATAAAAGTGCAAAGCTTGTG
GATGGAGTAAAGGTTGGAAAGACAGACGTTTATGTTGGCAATGAGAAAGTTGGAGAACGGAATTTATTTTATAGTAAACG
AAAGTTAGTAGCTACAACGGGAATGTACTGGAATAATGTGAAAGAAATTTTTTCTTACATGATAGGTGTTGGGAACGATG
GTTAA

Upstream 100 bases:

>100_bases
CTGCGTCTTTTTTATTGGAAGGAGAGGGAGATTTCGTTCATATTTTAAAAGGCCAAGCATACACTTGTACAAAAAGTGCC
AAAAGGACGTGGGGGGAAAT

Downstream 100 bases:

>100_bases
TCTCGTATGGGTAGCGATGGCTGTCATTGGGATTGTGTATGCGATGATAAATGGAACAATGGAAGAAATAAATAAAGCTG
TATTTGATGGAGCGAAAGAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRRICVIITLLIMYASVIPIPTYAKMTSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVS
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGNDG

Sequences:

>Translated_374_residues
MRRICVIITLLIMYASVIPIPTYAKMTSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVS
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGNDG
>Mature_374_residues
MRRICVIITLLIMYASVIPIPTYAKMTSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVS
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGNDG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=374, Percent_Identity=29.9465240641711, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787062, Length=297, Percent_Identity=35.016835016835, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=32.824427480916, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082054, Length=214, Percent_Identity=26.6355140186916, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41479; Mature: 41479

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRICVIITLLIMYASVIPIPTYAKMTSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHCEEEEECCCCCEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKEMVSVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCHHHHHHCCCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGN
HHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCE
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCC
EEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNV
CCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCCEEEECCHHHHHH
KEIFSYMIGVGNDG
HHHHHHHHCCCCCC
>Mature Secondary Structure
MRRICVIITLLIMYASVIPIPTYAKMTSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHCEEEEECCCCCEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKEMVSVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCHHHHHHCCCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGN
HHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCE
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCC
EEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNV
CCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCCEEEECCHHHHHH
KEIFSYMIGVGNDG
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]