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The map label for this gene is afuA [C]

Identifier: 222095055

GI number: 222095055

Start: 1383104

End: 1384144

Strand: Direct

Name: afuA [C]

Synonym: BCQ_1395

Alternate gene names: 222095055

Gene position: 1383104-1384144 (Clockwise)

Preceding gene: 222095054

Following gene: 222095056

Centisome position: 26.53

GC content: 35.16

Gene sequence:

>1041_bases
ATGGTATTTTCGTTATTAATGGGGTGTGGTGCAAAGAAAGAAGAAAGTGCTGGGGCAAAAGTAAAAGATGATAAATTATC
TGGATCATTAACTGTTTACACAGCAATTGAAGAAGAACTTGTACCAATTTATCTTGATTCTTTTAAAAAGAAATATCCAG
ATGTGAAGCTGAATATTGTTCGTGATTCAACTGGAGTTATTACAGCAAAATTGCTAGCTGAAGGGAAAAATACACAAGCA
GATGTTGTATGGGGAACTGCAGCTTCAAGTCTATTAGCTTTAGATAAAAAAGATATGTTAAAAGGATACTCTCCAAAAGG
AGCGGATCGCGTTTTACCGCAATTTAAAGATGATAAACAACCAGAAAAATGGGTAGGAAATACTGCATTTATGACGGGGA
TTGCTATAAATAAAGAAGAATTAAAGAAGAAAAATTTACCGATGCCAGAATCATACGAAGACTTAACGAAACCAGAGTAT
AAAGGAACGCTTGTTATGCCACATCCAGCATCTTCTGGAACAGGATTTTTAACAGTTTCTGCATGGTTACAAATTATGGG
TGAAGATAAAGGCTGGGATTACATGAAGAAACTTCATGATAATATGGCAACTTATACTCATTCAGGTTCAAAACCAGCGA
AATTAGCGGGGGCGGGTGAATATCCAGTTGGTGTATCGATGGTTTATAGTGCTTTGAAAGAGAAACAAAAAGGTGCACCA
GTTGAAGTTGTATTGCCAAAAGAAGGATTAGGTTGGGAAGTAGAAGCGAACGCACTTATTAAAAAAGATAATGAAAAAAA
TGAAAAATTAGCGCAAGCATTTTTAGATTGGGCAATTACTGATGATGTAATGAAGTTATACTTCGAGAAAAATGGATTTG
CGACAATTAAAAATGATTATAAACTTCCAGACGGATTCCCTAAAGATGTGACGGAAAAGTTATACAAAAAGAACGACTTT
AAGTGGGCAGCAGAAAATCGTGACAAAATTTTAGAAAAATGGGAAAAAGAGTTTGGCCAAAAAGCAGAACCGAAAAAGTA
A

Upstream 100 bases:

>100_bases
CTGCTTTTACACTAGTTGTTTATATTTGAGTTGTACATAAGATTTGTAAAAGGAGAGAGAGACAGTGAAAAAAACAATCT
TTAAAGCTGTAGCAACTGGA

Downstream 100 bases:

>100_bases
GTGAGTGGGAGAGAGAAAAAATGAGCGAATATTTATCAATTCAACACATTCAAAAACAGTTTGATGCATTTACAGCGTTA
AAAGATATTTCTTTTACTGT

Product: iron ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIVRDSTGVITAKLLAEGKNTQA
DVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPMPESYEDLTKPEY
KGTLVMPHPASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMVYSALKEKQKGAP
VEVVLPKEGLGWEVEANALIKKDNEKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDYKLPDGFPKDVTEKLYKKNDF
KWAAENRDKILEKWEKEFGQKAEPKK

Sequences:

>Translated_346_residues
MVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIVRDSTGVITAKLLAEGKNTQA
DVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPMPESYEDLTKPEY
KGTLVMPHPASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMVYSALKEKQKGAP
VEVVLPKEGLGWEVEANALIKKDNEKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDYKLPDGFPKDVTEKLYKKNDF
KWAAENRDKILEKWEKEFGQKAEPKK
>Mature_346_residues
MVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIVRDSTGVITAKLLAEGKNTQA
DVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPMPESYEDLTKPEY
KGTLVMPHPASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMVYSALKEKQKGAP
VEVVLPKEGLGWEVEANALIKKDNEKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDYKLPDGFPKDVTEKLYKKNDF
KWAAENRDKILEKWEKEFGQKAEPKK

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38610; Mature: 38610

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIV
CHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEE
RDSTGVITAKLLAEGKNTQADVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQ
ECCCCEEEEEEHHCCCCCCEEEEECHHHHHHHHCCHHHHHCCCCCCCHHHCCCCCCCCCC
PEKWVGNTAFMTGIAINKEELKKKNLPMPESYEDLTKPEYKGTLVMPHPASSGTGFLTVS
HHHHCCCHHEEEEEEECHHHHHHCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCEEEEHH
AWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMVYSALKEKQKGAP
HHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCC
VEVVLPKEGLGWEVEANALIKKDNEKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDY
EEEEECCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
KLPDGFPKDVTEKLYKKNDFKWAAENRDKILEKWEKEFGQKAEPKK
CCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIV
CHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEE
RDSTGVITAKLLAEGKNTQADVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQ
ECCCCEEEEEEHHCCCCCCEEEEECHHHHHHHHCCHHHHHCCCCCCCHHHCCCCCCCCCC
PEKWVGNTAFMTGIAINKEELKKKNLPMPESYEDLTKPEYKGTLVMPHPASSGTGFLTVS
HHHHCCCHHEEEEEEECHHHHHHCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCEEEEHH
AWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMVYSALKEKQKGAP
HHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCC
VEVVLPKEGLGWEVEANALIKKDNEKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDY
EEEEECCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
KLPDGFPKDVTEKLYKKNDFKWAAENRDKILEKWEKEFGQKAEPKK
CCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]