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The map label for this gene is lutR [H]

Identifier: 222095035

GI number: 222095035

Start: 1366403

End: 1367059

Strand: Direct

Name: lutR [H]

Synonym: BCQ_1375

Alternate gene names: 222095035

Gene position: 1366403-1367059 (Clockwise)

Preceding gene: 222095034

Following gene: 222095036

Centisome position: 26.21

GC content: 36.23

Gene sequence:

>657_bases
ATGATTAAAAATGGTACGTTAAAACCTGGTGACAAACTACTTCCTGTACATCAATTAGCTGAGCAGTTTCGAGTTGGTAG
ATCTGCTGTTCGTGAAGCGCTAAGTGCGCTAAGAGCAATGGGCTTAATTGAGATGAAACAAGGAGAAGGTACATATGTGA
AGAACTTCGATTCTTCTTCATTAACAAAATCATTAAACAATAGATTATTGATGAAGAAAGAAGATATTTTGAATTTATTA
GAAGTACGAAAGGTGCTTGAAGTGGGGGCAGTTCGAGCAGCTGCGGCAAAACGTACGGAAGATAATTTGCAAAATATGAA
GCATTGGTTAGATGAAATGGCAAAGAGCGTTGGAGATGAAAAAGCTGGTGAAAAAGCAGATTTTCAGTTCCATATGGGAA
TTGCAGAGTCTTCGCATAACAACATTTTGCTTGAACTTATGAATCATGTTTCAGAGATGATTGCTGAAACGATTGGTGAA
TCAAGACGCATTATTTTATATGGTGAACAAACAACATCAGAACGACTTATAGAAGAGCATCAAGTTATATATGATGCAGT
GTTAAAGCAAGATGTAGAATTAGCGCAGCAAGCAATGCTAGATCATTTAACAAATGTAGAACATATTGTCACAGGTAGCA
ACGATATAAATTCTTAA

Upstream 100 bases:

>100_bases
TTTCAATTGTTGTTTGGAAGATACTGAGGTGATTTAACTGGAGTACAAATCTATTAAACCGAAAAAGATTTACGAAGAAG
TATCTGAAGCTATTTTAACA

Downstream 100 bases:

>100_bases
TTTAATCTTTTAAAAATCTAATTTTCTGATAAAATAGAGAGAGATTAAGTAAGCGTTTTCTTTAGAAGAGGGAGAGGATA
GGCCATGGTTGAGCCTATTT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MIKNGTLKPGDKLLPVHQLAEQFRVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNNRLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEDNLQNMKHWLDEMAKSVGDEKAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGE
SRRIILYGEQTTSERLIEEHQVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGSNDINS

Sequences:

>Translated_218_residues
MIKNGTLKPGDKLLPVHQLAEQFRVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNNRLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEDNLQNMKHWLDEMAKSVGDEKAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGE
SRRIILYGEQTTSERLIEEHQVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGSNDINS
>Mature_218_residues
MIKNGTLKPGDKLLPVHQLAEQFRVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNNRLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEDNLQNMKHWLDEMAKSVGDEKAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGE
SRRIILYGEQTTSERLIEEHQVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGSNDINS

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=213, Percent_Identity=31.924882629108, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790032, Length=210, Percent_Identity=28.0952380952381, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790780, Length=213, Percent_Identity=30.0469483568075, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1789353, Length=212, Percent_Identity=27.8301886792453, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994955, Length=205, Percent_Identity=28.780487804878, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789621, Length=217, Percent_Identity=25.3456221198157, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24448; Mature: 24448

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNGTLKPGDKLLPVHQLAEQFRVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHH
LTKSLNNRLLMKKEDILNLLEVRKVLEVGAVRAAAAKRTEDNLQNMKHWLDEMAKSVGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGESRRIILYGEQTTSERLIEEH
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
QVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGSNDINS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCC
>Mature Secondary Structure
MIKNGTLKPGDKLLPVHQLAEQFRVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHH
LTKSLNNRLLMKKEDILNLLEVRKVLEVGAVRAAAAKRTEDNLQNMKHWLDEMAKSVGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGESRRIILYGEQTTSERLIEEH
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
QVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGSNDINS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]