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The map label for this gene is dacF [H]

Identifier: 222095010

GI number: 222095010

Start: 1338856

End: 1340142

Strand: Direct

Name: dacF [H]

Synonym: BCQ_1350

Alternate gene names: 222095010

Gene position: 1338856-1340142 (Clockwise)

Preceding gene: 222095009

Following gene: 222095011

Centisome position: 25.68

GC content: 36.29

Gene sequence:

>1287_bases
ATGGTAAATTTTAAGAAAATTTTGGTTTTTATTACCGCTATTTGTTTGTTTTTTTTGACACCTATGACGTTACGTGCTGA
GACGAATGTTGGAGTGAATCCAGAACAAGTGGCACCACCACCTAAGGAAGGGCCAAATGTTTTTAGTCAGTTTGCAACTA
CGATTGATGCGAAAACTGGAGATATCTTGTATGATAAAAACGCACATCATCGTGCATTTCCGGCTAGTATGACGAAAGTG
TTAACGGCGATTTTACTTATGGAGCATACGAAACCAGAGGATCAATTTACGTTTTCACAATTAGCATTAGATCAAGAGAA
GAGTAATTATCAAATTGAGCTTCAACCTGGTGAGACGATTAATAGAAATACTGCACTTATGATTTTAATGGTGCTGAGTG
CAAATGATGTATCATATGCAATTGCAGAGCATATTGGCGGAAGTGTTGAGAATTTCGCTAATATGATGAATGAGAGAGCG
AAGCAATTAGGGGCTACTGATAGTCATTTTGTAACACCAAATGGATTGCATGATCCGAATCATTATACTACGCCATATGA
TATGGCTATGATTACGAGAGGGGTGCAAAAGTACCCTGAGATTTTGCAAGCGATGAATACGAAAAGGACGACAGTTACGA
CATCTAGGCAGACGGTGTCCATTTTTAATAAATCTACTTATTTTGAAAATCCATTTAGTATTGGCGGTAAGACAGGTTTT
ACAAATGAAGCGCGCAATACGCTCGTTCTATTAAATGAGAAGGATGGAAATCGTATTATAAATGTAGTAATGGCTTCTCA
AAGACCTGAAATTTATGAAGATTTAAAGCAGATGGCGGACTATTCTTTTGGTCAATTTGTGAAGCAAACTGTATTAGATA
AACATAGTTGGCATCAAAAGACAACGTATTTAAATAAAGATATTGATAGCGAGCTTGAAAAAAGTGCAGAAGTGATGCTT
AAGAAAGATGAAGGAAAAAATGTGAGGACGATTTTCCGTGCGGCTTCATTAGATAAAGATTCTTTGTATCATAAAGGAAT
TCATCGTGGTGAGGTAGTTGGGGCAGTTGATATCACAAAGAATAATCAAACAATTGCGACGGTTAATGTTCTTTCTAAAG
AAGATGTTACTTTTGCAATGCCTAAAAAAGATACGATCGAGCCTGAAGTAAATGAAGCAAACGTGAAAATAATAAGCATG
GGGATAGGTGCTGCCATATTATTTGGAGCGATTTTATTTGTAGTGTTACGCCGAAATACAAAAGGAATGAAATCAGAAGA
AAAATAA

Upstream 100 bases:

>100_bases
GTAAAAAACAGTGAATATTCTTCCAATTTAATACAAATTACATATAGATTGGTAGATTCAGTTTTTAAACTGTGAGAGTA
TAGTGAAAAGGGAGAGTCAT

Downstream 100 bases:

>100_bases
ATTACTAGTTAAAAGGCTGTGAAGAGACTTTGTCACAGCCTTTTTTATATGTTTGAAATAACTAATAATTGTAAATTTTC
TTATTGCATTTATGTATTGT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVNFKKILVFITAICLFFLTPMTLRAETNVGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIELQPGETINRNTALMILMVLSANDVSYAIAEHIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAEVML
KKDEGKNVRTIFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNEANVKIISM
GIGAAILFGAILFVVLRRNTKGMKSEEK

Sequences:

>Translated_428_residues
MVNFKKILVFITAICLFFLTPMTLRAETNVGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIELQPGETINRNTALMILMVLSANDVSYAIAEHIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAEVML
KKDEGKNVRTIFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNEANVKIISM
GIGAAILFGAILFVVLRRNTKGMKSEEK
>Mature_428_residues
MVNFKKILVFITAICLFFLTPMTLRAETNVGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIELQPGETINRNTALMILMVLSANDVSYAIAEHIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAEVML
KKDEGKNVRTIFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNEANVKIISM
GIGAAILFGAILFVVLRRNTKGMKSEEK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=375, Percent_Identity=24.8, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI87082022, Length=384, Percent_Identity=24.21875, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786851, Length=379, Percent_Identity=23.2189973614776, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082054, Length=224, Percent_Identity=26.7857142857143, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47939; Mature: 47939

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFKKILVFITAICLFFLTPMTLRAETNVGVNPEQVAPPPKEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCC
DILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIELQPGETI
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAEHIGGSVENFANMMNERAKQLGATDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
HYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQK
CCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TTYLNKDIDSELEKSAEVMLKKDEGKNVRTIFRAASLDKDSLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSKEDVTFAMPKKDTIEPEVNEANVKIISMGIGAAILFGAILFVVLRRNT
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
KGMKSEEK
CCCCCCCC
>Mature Secondary Structure
MVNFKKILVFITAICLFFLTPMTLRAETNVGVNPEQVAPPPKEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCC
DILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIELQPGETI
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAEHIGGSVENFANMMNERAKQLGATDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
HYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQK
CCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TTYLNKDIDSELEKSAEVMLKKDEGKNVRTIFRAASLDKDSLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSKEDVTFAMPKKDTIEPEVNEANVKIISMGIGAAILFGAILFVVLRRNT
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
KGMKSEEK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]